Authors:
Anass Ben Moula Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA), Avenue Ennasr, P.O. Box 415 Rabat Principal, 10090 Rabat, Morocco
Plant, Microbial, Marine, and Precision Agriculture Biotechnology Research Team, Department of Life Sciences, Polydisciplinary Faculty of Larache, Abdelmalek Essaadi University, 745 BP, 92004 Larache, Morocco

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Saad Salhi Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA), Avenue Ennasr, P.O. Box 415 Rabat Principal, 10090 Rabat, Morocco

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Abdellatif Rahim Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA), Avenue Ennasr, P.O. Box 415 Rabat Principal, 10090 Rabat, Morocco

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Mouad Chentouf Animal Production Unit, Regional Center for Agricultural Research of Tangier, National Institute for Agricultural Research (INRA), Avenue Ennasr, P.O. Box 415 Rabat Principal, 10090 Rabat, Morocco

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Marianne Raes Integrated Veterinary Research Unit, Narilis, University of Namur, Belgium

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Jean Loup Bister Unit of Molecular Physiology, Faculty of Medicine, Narilis, University of Namur, Belgium

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Bouchra El Amiri Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA), Avenue Ennasr, P.O. Box 415 Rabat Principal, 10090 Rabat, Morocco
African Sustainable Agriculture Research Institute (ASARI), Mohammed VI Polytechnic University (UM6P), 70000, Laayoune, Morocco

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https://orcid.org/0000-0003-3443-5988
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Abstract

This study was conducted in two steps to evaluate the influence of freezing methods and natural extracts on cryopreserved ram sperm quality. Initially, the research compared the effects of two freezing methods: liquid nitrogen (LN2) versus −80 °C, on post-thawed ram semen on total and progressive motilities and velocity parameters. Experiment I revealed no significant differences (P > 0.05) between the LN2 and −80 °C freezing methods, indicating similar effects on the analyzed parameters. Experiment II aimed to examine the influence of Spirulina platensis (SP) and Salvia verbenaca (SV) extracts added to egg yolk extender on cryopreserved sperm quality, utilizing the −80 °C freezing method. Various concentrations (1.25, 3.75, 6.25 and 8.75 µg*mL−1) of acetone (Ac-SP and Ac-SV) and hexanoic (Hex-SP), as well as methanolic (MeOH-SV) extracts, were added into the extender. A thorough assessment of post-thawed sperm quality parameters, encompassing motility, velocity parameters, viability, membrane integrity, abnormality and lipid peroxidation was conducted. The outcomes demonstrated that 1.25 and 3.75 g*mL−1 of Ac-SP and Hex-SP and 1.25 µg*mL−1 of AC-SV and MeOH-SV increased the post-thawed ram sperm quality. In conclusion, this study emphasizes the antioxidant properties of SP and SV extracts, highlighting their potential to protect cryopreserved sperm cells from oxidative stress at −80 °C.

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Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
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Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
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ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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