Aino virus infection is responsible for epizootic and/or sporadic outbreaks of abortions, stillbirths and premature delivery among pregnant ruminants. The epizootiology of Aino virus infection is poorly defined in South Korea, therefore our aim was to assess its seroprevalence among sheep and goats. We also wanted to evaluate management and regional risk factors that might influence the frequency of infection. Between 2012 and 2013, 26 of 331 flocks (7.9%) and 139 of 915 heads (15.2%) were found serologically positive. In 2018, when samples were again collected in the same regions, 35 of 308 flocks (11.4%) and 89 of 735 heads (12.1%) showed serum-neutralising antibodies against Aino virus. Our results revealed that the age class and history of reproductive problems in the flocks are connected to an increased risk of being positive. The management risk factor attributes showed that preventive measures, such as the routine application of insecticide in farms, decreased the odds for seropositivity to Aino virus (OR = 0.453, P = 0.001). We observed a significant difference in the individual likelihood of being positive in the southern and western provinces with respect to that in the northern and eastern provinces, respectively (OR = 2.199, P < 0.001 and OR = 2.177, P < 0.001). The results of this study may serve as a basis for future epizootic studies on Aino virus infection in South Korea.
Brenner, J. , Tsuda, T. , Yadin, H. , Chai, D. , Stram, Y. and Kato, T. (2004): Serological and clinical evidence of a teratogenic Simbu serogroup virus infection of cattle in Israel, 2001–2003. Vet. Ital. 40, 119–123.
Coverdale, O. R. , Cybinski, D. H. and St. George, T. D. (1978): Congenital abnormalities in calves associated with Akabane virus and Aino virus. Aust. Vet. J. 54, 151–152.
Doherty, R. L. (1972): Arboviruses of Australia. Aust. Vet. J. 48, 172–180.
Donald, A. and Donner, A. (1987): Adjustments to the Mantel-Haenszel chi-square statistic and odds ratio variance estimator when the data are clustered. Stat. Med. 6, 491–499.
Humphry, R. W. , Cameron, A. and Gunn, G. J. (2004): A practical approach to calculate sample size for herd prevalence surveys. Prev. Vet. Med. 65, 173–188.
Iwamoto, J. and Furukawa, M. (2020): The estimation of duration of maternally-derived antibodies against Akabane, Aino, and Chuzan virus in calves by the receiver operating characteristic analysis. J. Vet. Med. Sci. 82, 1614–1618.
Jun, K. , Yanaka, T. , Lee, K. K. and Lee, J. B. (2018): Seroprevalence of bovine arboviruses belonging to genus Orthobunyavirus in South Korea. J. Vet. Med. Sci. 80, 1619–1623.
Kim, Y. H. , Kweon, C. H. , Tark, D. S. , Lim, S. I. , Yang, D. K. , Hyun, B. H. , Song, J. Y. , Hur, W. and Park, S. C. (2011): Development of inactivated trivalent vaccine for the teratogenic Aino, Akabane and Chuzan viruses. Biologicals 39, 152–157.
Kim, Y. H. , Oem, J. K. , Lee, E. Y. , Lee, K. K. , Kim, S. H. , Lee, M. H. and Park, S. C. (2015): Seroprevalence of five arboviruses in sentinel cattle as part of nationwide surveillance in South Korea, 2009–2012. J. Vet. Med. Sci. 77, 247–250.
Lim, S. I. , Kweon, C. H. , Tark, D. S. , Kim, S. H. and Yang, D. K. (2007): Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea. J. Vet. Sci. 8, 45–49.
Lim, S. I. , Kweon, C. H. , Yang, D. K. , Tark, D. S. and Kweon, J. H. (2005): Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus. J. Vet. Sci. 6, 251–254.
Livestock Management Division (2012): Domestic Animals Statistics. Animal Farm Policy Bureau, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.
Melaku, S. K. , Regassa, F. , Tessema, T. S. , Dawo, F. , Oguma, K. , Nagayama, K. and Sentsui, H. (2016): Serological survey of viral diseases relating to reproductive failure among Artiodactyla in Ethiopian Camelus dromedarius .Microbiol. Immunol. 60, 506–510.
Miura, Y. , Inaba, Y. , Tsuda, T. , Tokuhisa, S. , Sato, K. , Akashi, H. and Matumoto, M. (1982): A survey of antibodies to arthropod-borne viruses in Indonesian cattle. Nihon Juigaku Zasshi 44, 857–863.
Norton, J. H. , Tranter, W. P. and Campbell, R. S. (1989): A farming systems study of abortion in dairy cattle on the Atherton Table l and. 2. The pattern of infectious diseases. Aust. Vet. J. 66, 163–167.
Parsonson, I. M. and McPhee, D. A. (1985): Bunyavirus pathogenesis. In: Maramorosch, K , Murphy, F. A. and Shatkin, A. J. (eds) Advances in Virus Research, vol. 30. Academic Press, Orlando. pp. 279–316.
Porterfield, J. S. and Della-Porta, A. J. (1981): Bunyaviridae: infections and diagnosis. In: Kurstak, E. and Kurstak, C. (eds) Comparative Diagnosis of Viral Diseases, vol. IV. Academic Press, New York. pp. 479–508.
Reiczigel, J. , Földi, J. and Ózsvári, L. (2010): Exact confidence limits for prevalence of a disease with an imperfect diagnostic test. Epidemiol. Infect. 138, 1674–1678.
Sanders, C. J. , Harrup, L. E. , Tugwell, L. A. , Brugman, V. A. , England, M. and Carpenter, S. (2017): Quantification of within- and between-farm dispersal of Culicoides biting midges using an immunomarking technique. J. Appl. Ecol. 54, 1429–1439.
Sergeant, E. S. G. (2018): Epitools Epidemiological Calculators. Ausvet. Available at: http://epitools.ausvet.com.au.
Sugiyama, I. , Shimizu, E. , Nogami, S. , Suzuki, K. , Miura, Y. and Sentsui, H. (2009): Serological survey of arthropod-borne viruses among wild boars in Japan. J. Vet. Med. Sci. 71, 1059–1061.
Wang, J. , Firth, C. , Amos-Ritchie, R. , Davis, S. S. , Yin, H. , Holmes, E. C. , Blasdell, K. R. and Walker, P. J. (2019): Evolutionary history of Simbu serogroup orthobunyaviruses in the Australian episystem. Virology 535, 32–44.
Yang, D. K. , Hwang, I-J. , Kim, B-H. , Kweon, C-H. , Lee, K-W. , Kang, M-I. , Lee, C-S and Cho, K-O. (2008a): Serosurveillance of viral diseases in Korean native goats (Capra hircus). J. Vet. Med. Sci. 70, 977–979.
Yang, D. K. , Kim, B-H. , Kweon, C-H. , Nah, J-J. , Kim, H-J. , Lee, K-W. , Yang, Y-J. and Mun, K-W. (2008b): Serosurveillance for Japanese encephalitis, Akabane, and Aino viruses for Thoroughbred horses in Korea. J. Vet. Sci. 9, 381–385.
Yoshida, K. , Ohashi, S. , Kubo, T. and Tsuda, T. (2000): Comparison of intertypic antigenicity of Aino virus isolates by dot immunobinding assay using neutralizing monoclonal antibodies. J. Clin. Microbiol. 38, 4211–4214.