The new hot water percolation (HWP) method was introduced to determine the phosphorus supply of soils from the Soil Bank of 36 Hungarian soils. The present work aimed to explain the availability of phosphorus by determining the inorganic phosphate fractions and using ryegrass test plants. Four inorganic phosphate fractions were distinguished: Fraction I, the sorbed phosphates; Fraction II, the easily soluble Ca phosphates and the Al bound phosphates; Fraction III, the Fe phosphates; and Fraction IV, the hardly soluble Ca phosphates. Fraction II, in which the easily soluble Ca phosphates and Al phosphates accumulate, was the main phosphorus source for the test plants on both calcareous and non-calcareous soils. Fraction III (the iron phosphates) plays a greater role in non-calcareous soils, while Fraction IV (the hardly soluble Ca phosphates) in calcareous soils. Both fractions are closely connected with soil development, and with soil properties such as pH and CaCO 3 content. The hot water percolation method reflects the phosphorus supply of soil as well as that measured with ryegrass plants and with the AL method. This new HWP method is in good correlation with the main source of phosphate, with fraction II. For routine purposes the first collected HWP fraction can possibly be used to determine the phosphorus supply of soil correlating well with the phosphorus uptake of test plants.