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Cotyledon explants of tomato (Lycopersicum esculentum Mill.) cv. Kecskeméti 262 were excised from 9-day-old in vitro seedlings. The transformation method of Arrillaga et al. (1998) was tested and found to be effective for the introduction of foreign genes into this variety. Using an Agrobacterium strain carrying the plasmid 35S GUS INT, ten independent transgenic lines retaining all the phenotypic markers of cv. Kecskeméti 262 were isolated on regeneration medium containing kanamycin and were tested further for ß -glucuronidase activity. The results show that the method of Arrillaga et al. (1998) is suitable for the introduction of agronomically important genes into the cultivar Kecskeméti 262.

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