Acta Agronomica Hungarica
Volume/Issue:
Volume 50: Issue 2
Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves
Authors:
J. Dobránszki Research Centre of the University of Debrecen Nyíregyháza, Hungary

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K. Magyar-Tábori Research Centre of the University of Debrecen Research Centre Nyíregyháza Nyíregyháza, Hungary

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E. Jámbor-Benczúr Szent István University Budapest, Hungary

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et al. Szent István University Budapest, Hungary

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Pages:
117–126
Online Publication Date:
22 Jul 2005
Publication Date:
01 Jul 2002
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.50.2002.2.1
Keywords:
meta-topolin; meta-topolin; meta-topolin; meta-topolin; conditioning; anatomical structure; organogenetic index
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The effects of a new type of aromatic cytokinin, meta-topolin, on the morphology and histology of apple leaves and its post-effects on the subsequent shoot regeneration from in vitro leaves were studied in cv. Royal Gala. The media applied for pre-treatment differed from each other in their cytokinin composition: medium No. 1 contained no cytokinin, No. 2 was supplemented with 0.5 mg l-1 benzyladenine, while Nos. 3-6 contained meta-topolin, the new type of cytokinin, in four concentrations (0.5-1.0-1.5-2.0 mg l-1). After a 3-week pre-treatment on these media shoot regeneration was induced on two test regeneration media containing thidiazuron (0.2 mg l-1) or benzyladenine (5.0 mg l-1). Irrespective of the pre-treatments, high regeneration (97-100%) was observed on all the regeneration media. however, the conditioning of apple shoots for three weeks on medium supplemented with meta-topolin in a concentration range between 0.5 and 1.5 mg l-1 caused a significant decrease in the rate of vitrified shoots (down to 13.4%) and increased the number of regenerated shoots per leaf segment significantly (up to 15.1). There was a positive correlation between the histological status and regeneration capacity of in vitro leaves. According to these results, meta-topolin, as a new source of cytokinin, could increase the morphogenic potential of apple leaves.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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