Author: G. Galiba 1
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  • 1 Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary
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The location of major QTLs or even genes controlling abiotic stress tolerance is now possible by the application of marker-mediated techniques. This is achieved by exploiting precise genetic stocks, such as doubled haploids (DHs), recombinant substitution lines (RSLs) and recombinant inbred lines (RILs), along with the comprehensive genetic maps now available through the application of molecular marker techniques. These strategies are illustrated here showing how QTLs/genes affecting vernalization response, cold tolerance, osmotic adjustment, osmolite accumulation (free amino acids, polyamines and carbohydrates), salt tolerance and cold-regulated protein accumulation have been identified and located. Also, an example of marker-assisted selection (MAS) for frost tolerance is presented. Major loci and QTLs affecting stress tolerance in Triticeae have been mapped on the group 5 chromosomes, where the highest concentration of abiotic stress-related QTLs (vernalization response, frost tolerance, salt tolerance and osmolite accumulation) was located. A conserved region with a major role in osmotic adjustment has been located on the group 7 chromosomes.

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