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  • 1 Agricultural Research Institute of the Hungarian Academy of Science Martonvásár, Hungary
  • | 2 Agricultural Research Institute of the Hungarian Academy of Science Martonvásár, Hungary
  • | 3 Agricultural Biotechnology Centre Gödöllő, Hungary
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The first steps in studies on the female and male gametophytes of wheat involved the light microscope analysis of semi-thin sections of embryo sacs containing egg-cells developing in planta. The information thus obtained on the development of the egg-cell from its initial formation to maturity contributed to the successful isolation of egg-cells. The morphological and ultrastructural details of egg-cells isolated 3, 6, 9, 12, 15 and 18 days after emasculation were examined to determine the function of the female gametophyte and its suitability for micromanipulation. A sufficient number of gametoplasts in the right stage of development are required for the successful microinjection and in vitro fusion of egg-cells and male gametes. No data are yet available on the fusiogenicity of wheat egg-cells in various stages of development. Various in vitro fertilisation and microinjection techniques could be of service in gamete fusion experiments aimed at the creation of interspecific and intergeneric hybrids which do not occur in nature due to sporophytic incompatibility. The results acquired in investigations on immature embryos can be used for the study of embryos developing from egg-cells fertilised in vitro.

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Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation
1950
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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