Acta Agronomica Hungarica
Volume/Issue:
Volume 51: Issue 3
Response of Vicia faba plants to the interactive effect of sodium chloride salinity and salicylic acid treatment
Author:
A. A. Tammam Botany Department, Faculty of Science, Alexandria University Alexandria, Egypt

Search for other papers by A. A. Tammam in
Current site
Google Scholar
PubMed Close
Pages:
239–248
Online Publication Date:
22 Jul 2005
Publication Date:
01 Nov 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.51.2003.3.1
Keywords:
amino acids; amino acids; amino acids; proteins; salicylic acid; Vicia faba; NaCl; proline; mineral contents
Restricted access

The leaf area, fresh and dry matter, and water content in the roots and shoots of broad bean were significantly reduced with a rise in salinity. The protein components in the roots and shoots decreased in response to salinity, whereas the proline content significantly increased. The sodium content in both roots and shoots increased with increasing salinity, whereas potassium and calcium decreased. Salinity induced an increase in total amino acids and ammonia. Spraying with salicylic acid (SA) increased the three growth parameters, stimulated the synthesis of protein at all salinities, retarded the accumulation of proline, retarded the accumulation of Na+ and increased the content of K+ and Ca2+. The total content of amino acids was about 1.6-fold the untreated control and there was a drastic increase in the content of threonine and serine. The electrophoretic pattern of SA-treated seedlings showed 21 polypeptides compared to 12 in the salt-treated ones. Salinity plus SA resulted in the disappearance of 4 polypeptides. In addition, two peptides with molecular masses of 99 and 102 kDa appeared in the gel in both NaCl-treated seedlings and NaCl+SA-treated seedlings.

  • Larque-Saavedra, A. 1978: The anti-transpirant effect of acetylsalicylic acid on Phaseolus vulgaris L. Physiol. Plant., 43, 126-128.

    'The anti-transpirant effect of acetylsalicylic acid on Phaseolus vulgaris L. ' () 43 Physiol. Plant. : 126 -128 .

    • Search Google Scholar

  • Ahmed, A. M., Ismail, A. M., Azzoz, M. M. 2001: Protein patterns in germinating seeds of Vicia faba lines in response to interactive effects of salinity and vitamin treatments. Phyton, 41, 79-110.

    'Protein patterns in germinating seeds of Vicia faba lines in response to interactive effects of salinity and vitamin treatments. ' () 41 Phyton : 79 -110 .

    • Search Google Scholar

  • Allen, S., Grimshaw, H. M., Parkinson, J. A., Quarmby, C. 1974: Chemical Analysis of Ecological Materials. Blackwell Sci. Publ., Oxford, London.

    Chemical Analysis of Ecological Materials , ().

  • Skriver, K., Mundy, J. 1990: Gene expression in response to abscisic acid and osmotic stress. Plant Cell, 2, 503-512.

    'Gene expression in response to abscisic acid and osmotic stress. ' () 2 Plant Cell : 503 -512 .

    • Search Google Scholar

  • Smirnoff, N., Cumbes, Q. J. 1989: Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry, 28, 1057-1060.

    'Hydroxyl radical scavenging activity of compatible solutes. ' () 28 Phytochemistry : 1057 -1060 .

    • Search Google Scholar

  • Speckman, D. H., Stein, W. H., Moore, S. S. 1958: Automatic recording apparatus for use in chromatography for amino acids. Analyt. Chem., 30, 1090-1205.

    'Automatic recording apparatus for use in chromatography for amino acids. ' () 30 Analyt. Chem. : 1090 -1205 .

    • Search Google Scholar

  • Venekamp, J. H. 1989: Regulation of cytosol acidity in plants under conditions of drought. Physiol. Plant., 76, 112-117.

    'Regulation of cytosol acidity in plants under conditions of drought. ' () 76 Physiol. Plant. : 112 -117 .

    • Search Google Scholar

  • Kononowicz, A. K., Nelson, D. E., Singh, N. K., Hasegawa, P. M., Bressan, R. A. 1992: Regulation of the osmotin gene promoter. Plant Cell, 4, 513-524.

    'Regulation of the osmotin gene promoter. ' () 4 Plant Cell : 513 -524 .

  • Laemmli, M. K. 1970: Cleavage of structural protein during assembly of the head bacteriophage T4. Nature, 277, 680-685.

    'Cleavage of structural protein during assembly of the head bacteriophage T4. ' () 277 Nature : 680 -685 .

    • Search Google Scholar

  • Weber, K., Osborn, M. 1969: The reliability of molecular weight determinations by dodecyl sulphate polyacrylamide gel electrophoresis. Biol. Chem., 244, 4405-4412.

    'The reliability of molecular weight determinations by dodecyl sulphate polyacrylamide gel electrophoresis. ' () 244 Biol. Chem. : 4405 -4412 .

    • Search Google Scholar

  • Zidan, I., Azaizeh, H., Neumann, P. M. 1990: Does salinity reduce growth in maize root epidermal cells by inhibiting their capacity for cell wall acidification? Plant Physiol., 93, 7-11.

    'Does salinity reduce growth in maize root epidermal cells by inhibiting their capacity for cell wall acidification? ' () 93 Plant Physiol. : 7 -11 .

    • Search Google Scholar

  • Handa, S., Bressan, R. A., Handa, A. K., Carpila, N. C., Hasegawa, P. M. 1983: Solute contributing to osmotic adjustment in cultured plant cells adapted to water stress. Plant Physiol., 73, 834-843.

    'Solute contributing to osmotic adjustment in cultured plant cells adapted to water stress. ' () 73 Plant Physiol. : 834 -843 .

    • Search Google Scholar

  • Harrak, H., Azelmat, S., Baker, E. N., Tabaeizadeh, Z. 2001: Isolation and characterization of a gene encoding a drought-induced cysteine protease in tomato (Lycopersicon esculentum). Genome, 44, 368-374.

    'Isolation and characterization of a gene encoding a drought-induced cysteine protease in tomato ' () 44 (Lycopersicon esculentum). Genome : 368 -374 .

    • Search Google Scholar

  • Hartree, E. F. 1972: A modification of Lowry method that gives a linear photometric response. Anal. Biochem., 48, 422-425.

    'A modification of Lowry method that gives a linear photometric response. ' () 48 Anal. Biochem. : 422 -425 .

    • Search Google Scholar

  • Ayoub, A., Khalil, M., Grace, J. 1992: Acclimation to drought in Acer pseudoplatanus L. (sycamore) seedlings. J. Exp. Bot., 43, 1591-1602.

    'Acclimation to drought in Acer pseudoplatanus L. (sycamore) seedlings. ' () 43 J. Exp. Bot. : 1591 -1602 .

    • Search Google Scholar

  • Bolarin, M. C., Santa-Cruz, A., Cayuela, E., Perez-Alfocea, F. 1995: Short-term solute changes in leaves and roots of cultivated and wild tomato seedlings under salinity. J. Plant Physiol., 147, 463-468.

    'Short-term solute changes in leaves and roots of cultivated and wild tomato seedlings under salinity. ' () 147 J. Plant Physiol. : 463 -468 .

    • Search Google Scholar

  • Chang, S., Puryear, J. D., Dilp, L., Dias, M. A., Funkhouser, E. A., Newlon, R. J., Cairney, J. 1996: Gene expression under water deficit in loblolly pine (Pinus taeda): Isolation and characterization of CDNA clones. Physiol. Plant., 97, 139-148.

    'Gene expression under water deficit in loblolly pine (Pinus taeda): Isolation and characterization of CDNA clones. ' () 97 Physiol. Plant. : 139 -148 .

    • Search Google Scholar

  • Chao, W. S., Gu, Y. Q., Pautot, V., Bray, E. A., Walling, L. L. 1999: Leucine aminopeptidase RNAs, proteins and activities increase in response to water deficit, salinity and the wound signals systemin, methyl jasmonate and abscisic acid. Plant Physiol., 120, 979-992.

    'Leucine aminopeptidase RNAs, proteins and activities increase in response to water deficit, salinity and the wound signals systemin, methyl jasmonate and abscisic acid. ' () 120 Plant Physiol. : 979 -992 .

    • Search Google Scholar

  • Chiang, H. H., Dandekar, A. M. 1995: Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and response to desiccation. Plant Cell Environ., 18, 1280-1290.

    'Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and response to desiccation. ' () 18 Plant Cell Environ. : 1280 -1290 .

    • Search Google Scholar

  • Barkosky, R. R., Einhellig, F. A. 1993: Effects of salicylic acid on plant-water relationship. J. Chem. Ecol., 41, 237-247.

    'Effects of salicylic acid on plant-water relationship. ' () 41 J. Chem. Ecol. : 237 -247 .

    • Search Google Scholar

  • Bates, S., Waldren, R. R., Teare, I. D. 1973: Rapid determination of free proline for water stress studies. Plants and Soil, 39, 205-207.

    'Rapid determination of free proline for water stress studies. ' () 39 Plants and Soil : 205 -207 .

    • Search Google Scholar

  • Blum, A., Ebercon, A. 1976: Genotypic responses in sorghum to drought stress. III. Free proline accumulation and drought resistance. Crop Sci., 16, 428-431.

    'Genotypic responses in sorghum to drought stress. III. Free proline accumulation and drought resistance. ' () 16 Crop Sci. : 428 -431 .

    • Search Google Scholar

  • Clifford, S. C., Arndt, S. K., Corlett, J. E., Sangeela, J., Sankhla, N., Popp, M., Jenes, H. G. 1998: The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lomk). J. Exp. Bot., 49, 967-977.

    'The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lomk). ' () 49 J. Exp. Bot. : 967 -977 .

    • Search Google Scholar

  • Cordovilla, M. P., Ligero, F., Liuch, C. 1994: The effect of salinity on N fixation and assimilation in Vicia faba. J. Exp. Bot., 45, 1483-1488.

    'The effect of salinity on N fixation and assimilation in ' () 45 Vicia faba. J. Exp. Bot. : 1483 -1488 .

    • Search Google Scholar

  • Flowers, T. J., Yeo, A. R. 1992: Solute Transport in Plants. Chapman and Hall, London.

    Solute Transport in Plants , ().

  • Greenway, H., Munns, R. 1980: Mechanisms of salt tolerance in non halophytes. Ann. Rev. Plant Physiol., 31, 49-90.

    'Mechanisms of salt tolerance in non halophytes. ' () 31 Ann. Rev. Plant Physiol. : 49 -90 .

    • Search Google Scholar

  • Gupta, S. D. 1999: Protein profiles of somatic embryos and regenerated plants from NaCl selected and control cultures of orchard grass. Biol. Plant., 42, 297-302.

    'Protein profiles of somatic embryos and regenerated plants from NaCl selected and control cultures of orchard grass. ' () 42 Biol. Plant. : 297 -302 .

    • Search Google Scholar

  • Hames, B. D., Rickwood, D. 1990: An introduction to polyacrylamide gel electrophoresis. In: Hames, B. D., Rickwood, D.(eds.), Electrophoresis of Proteins. A Practical Approach. England Publishing Co. TRL, London. pp. 34-48.

    An introduction to polyacrylamide gel electrophoresis , () 34 -48 .

  • Shalaby, E. E., Epstein, E., Qual Set, C. O. 1993: Variation in salt tolerance among some wheat and triticale genotypes. Agr. and Crop Sci., 171, 298-304.

    'Variation in salt tolerance among some wheat and triticale genotypes. ' () 171 Agr. and Crop Sci. : 298 -304 .

    • Search Google Scholar

  • Silhavy, D., Hutvagner, G., Barta, E., Banfalvi, Z. 1995: Isolation and characterization of water-stress-inducible clone from Solanum chacoense. Plant Mol. Biol., 43, 587-595.

    'Isolation and characterization of water-stress-inducible clone from ' () 43 Solanum chacoense. Plant Mol. Biol. : 587 -595 .

    • Search Google Scholar

  • Marcelis, L. F. M., van Hooijdonk, J. 1999: Effect of salinity on growth, water use and nutrient use in radish (Raphanus sativus L.). Plant and Soil, 215, 57-64.

    'Effect of salinity on growth, water use and nutrient use in radish (Raphanus sativus L.). ' () 215 Plant and Soil : 57 -64 .

    • Search Google Scholar

  • Marschner, H. 1995: Mineral Nutrition of Higher Plants. 2nd ed. Academic Press, London.

    Mineral Nutrition of Higher Plants , ().

  • Morales, M. A., Sanchez-Blanco, M. J., Olmoss, E., Torrecillas, S. A., Alarcen, J. J. 1998: Changes in the growth, leaf water relations and cell ultrastructure in Argyranthemum coronopifollium plants under saline conditions. J. Plant Physiol., 153, 174-180.

    'Changes in the growth, leaf water relations and cell ultrastructure in Argyranthemum coronopifollium plants under saline conditions. ' () 153 J. Plant Physiol. : 174 -180 .

    • Search Google Scholar

  • Pancheva, T. V., Popova, L. P., Uzunova, A. N. 1996: Effect of salicylic acid on growth and photosynthesis in barley plants. Plant Physiol., 149, 57-63.

    'Effect of salicylic acid on growth and photosynthesis in barley plants. ' () 149 Plant Physiol. : 57 -63 .

    • Search Google Scholar

  • Pierpoint, W. S. 1994: Salicylic acid and its derivatives in plants, medicine, metabolities and messenger moelcules. Bot Res., 20, 163-235.

    'Salicylic acid and its derivatives in plants, medicine, metabolities and messenger moelcules. ' () 20 Bot Res. : 163 -235 .

    • Search Google Scholar

  • Raskin, I. 1992: Role of salicylic acid in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 43, 439-463.

    'Role of salicylic acid in plants. ' () 43 Annu. Rev. Plant Physiol. Plant Mol. Biol. : 439 -463 .

    • Search Google Scholar

  • Singh, N. K., Bracker, C. A., Hasegawa, P. M., Handa, A. K., Buckel, S., Herodson, M. A., Pfankoch, E., Regrier, F. E., Bressan, R. A. 1989: Characterization of osmotin: a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol., 85, 529-536.

    'Characterization of osmotin: a thaumatin-like protein associated with osmotic adaptation in plant cells. ' () 85 Plant Physiol. : 529 -536 .

    • Search Google Scholar

  • Singh, N. K., Nelson, D., Kutne, D., Hasegawa, P. M., Bressan, R. A. 1987: Molecular cloning of osmotin and adaptation to low regulation of its expression by ABA and low water potential. Plant Physiol., 190, 1090-1101.

    'Molecular cloning of osmotin and adaptation to low regulation of its expression by ABA and low water potential. ' () 190 Plant Physiol. : 1090 -1101 .

    • Search Google Scholar

  • Lopez-Delgado, H., Dat, J. F., Foyer, C. H., Scott, I. M. 1998: Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2. J. Exp. Bot., 49, 11-19.

    'Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2 ' () 49 J. Exp. Bot. : 11 -19 .

    • Search Google Scholar

  • Lutts, S., Kinet, J. M., Bouharmont, J. 1996: Effect of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. Plant Growth Regulation, 19, 207-218.

    'Effect of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. ' () 19 Plant Growth Regulation : 207 -218 .

    • Search Google Scholar

  • Reviron, M. P., Vartanian, N., Sallanlin, M., Huet, J. C., Pernollet, J. C., de Vienne, D. 1992: Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves. Plant Physiol., 100, 1486-1493.

    'Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves. ' () 100 Plant Physiol. : 1486 -1493 .

    • Search Google Scholar

  • Rey, P., Pruvot, G., Becuwe, N., Eymery, F., Rumeau, D., Pelitier, G. 1998: A novel thioredoxin-like protein located in the chloroplast is induced by water deficit in Solanum tuberosum L. plants. Plant J., 13, 79-107.

    'A novel thioredoxin-like protein located in the chloroplast is induced by water deficit in Solanum tuberosum L. plants. ' () 13 Plant J. : 79 -107 .

    • Search Google Scholar

  • Schobert, B., Tschesche, H. 1978: Unusual solution properties of proline and its interaction with proteins. Biochem. Biophys. Acta, 541, 270-277.

    'Unusual solution properties of proline and its interaction with proteins. ' () 541 Biochem. Biophys. Acta : 270 -277 .

    • Search Google Scholar

  • Shaddad, M. A., El-Tayeb, M. A. 1990: Interactive effect of soil moisture content and hormonal treatment on dry matter and pigment contents of some crop plants. Acta Agron. Hung., 39, 49-57.

    'Interactive effect of soil moisture content and hormonal treatment on dry matter and pigment contents of some crop plants. ' () 39 Acta Agron. Hung. : 49 -57 .

    • Search Google Scholar

  • Shaddad, M. A., Radi, A. F., Abd El-Rahaman, A. M., Azzoz, M. M. 1990: Responses of seeds of Lupinus termis and Vicia faba to the interactive effect of salinity and ascorbic acid or pyridoxine (B6). Plant and Soil, 122, 177-183.

    'Responses of seeds of Lupinus termis and Vicia faba to the interactive effect of salinity and ascorbic acid or pyridoxine (B6). ' () 122 Plant and Soil : 177 -183 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 36 0 0
Dec 2024 28 0 0
Jan 2025 100 0 0
Feb 2025 89 0 0
Mar 2025 96 0 0
Apr 2025 32 0 0
May 2025 0 0 0