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  • 1 Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár, Hungary
  • 2 Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár, Hungary
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The relationship between ultrastructural changes in the anthers caused by cold pretreatment and the haploid induction capacity of different maize genotypes was investigated. The degeneration of the tapetal cells appears to be genotype-dependent, but the extent of the degeneration is not correlated with the androgenetic ability of the given genotype. Based on the stainability of the cytoplasm, two microspore types were found. The results suggest that the “dense” microspores take part in pollen embryogenesis.

  • Tyagi, A. K., Rashid, A., Maheshwary, S. C. 1979: High frequency production of embryos in Datura inoxia from isolated pollen grains by combined cold treatment and serial culture of anthers in liquid medium. Protoplasma, 99, 11-17.

    'High frequency production of embryos in Datura inoxia from isolated pollen grains by combined cold treatment and serial culture of anthers in liquid medium. ' () 99 Protoplasma : 11 -17.

    • Search Google Scholar
  • Wei, Z. M., Kyo, M., Harada, H. 1986: Callus formation and plant regeneration through direct culture of isolated pollen of Hordeum vulgare cv. Sabarlis. Theor. Appl. Genet., 72, 252-255.

    'Callus formation and plant regeneration through direct culture of isolated pollen of Hordeum vulgare cv. Sabarlis. ' () 72 Theor. Appl. Genet. : 252 -255.

    • Search Google Scholar
  • Wenzel, G., Thomas, E. 1974: Observations on the growth in culture of anthers of Secale cereale. Z. Pflanzenzücht., 72, 89-94.

    'Observations on the growth in culture of anthers of ' () 72 Secale cereale. Z. Pflanzenzücht. : 89 -94.

    • Search Google Scholar
  • Wilson, H. M., Mix, G., Foroughi-Wehr, B. 1978: Early microspore divisions and subsequent formation of microspore callus at high frequency in anthers of Hordeum vulgare L. J. Exp. Bot., 29, 227-238.

    'Early microspore divisions and subsequent formation of microspore callus at high frequency in anthers of Hordeum vulgare L. ' () 29 J. Exp. Bot. : 227 -238.

    • Search Google Scholar
  • Zaki, M. A. M., Dickinson, H. G. 1990: Structural changes during the first division of embryos resulting from anther and free microspore culture in Brassica napus. Protoplasma, 156, 149-162.

    'Structural changes during the first division of embryos resulting from anther and free microspore culture in ' () 156 Brassica napus. Protoplasma : 149 -162.

    • Search Google Scholar
  • Spurr, A. R. 1969: A low viscosity resin embedding medium for electron microscopy. J. Ultrastr. Res., 26, 31-43.

    'A low viscosity resin embedding medium for electron microscopy. ' () 26 J. Ultrastr. Res. : 31 -43.

    • Search Google Scholar
  • Sunderland, N. 1978: Strategies in the improvement of yields in anther culture. In, Proceedings of Symposium on Plant Tissue Culture. Science Press, Peking, pp. 65-86.

    Strategies in the improvement of yields in anther culture , () 65 -86.

  • Sunderland, N., Roberts, M. 1979: Cold pretreatment of excised flower buds in float culture of tobacco anthers. I. J. Exp. Bot., 43, 405-414.

    'Cold pretreatment of excised flower buds in float culture of tobacco anthers. I. ' () 43 J. Exp. Bot. : 405 -414.

    • Search Google Scholar
  • Sunderland, N., Roberts, M., Xu, Z. H. 1982: Shed pollen culture in Hordeum vulgare. J. Exp. Bot., 33, 1086-1095.

    'Shed pollen culture in ' () 33 Hordeum vulgare. J. Exp. Bot. : 1086 -1095.

  • Alexander, M. P. 1969: Differential staining of aborted and nonaborted pollen. Stain Technol., 44, 117-122.

    'Differential staining of aborted and nonaborted pollen. ' () 44 Stain Technol. : 117 -122.

    • Search Google Scholar
  • Barloy, D., Denis, L., Beckert, M. 1989: Comparison of the aptitude for anther culture in some androgenic doubled haploid maize lines. Maydica, 34, 303-308.

    'Comparison of the aptitude for anther culture in some androgenic doubled haploid maize lines. ' () 34 Maydica : 303 -308.

    • Search Google Scholar
  • Cadic, A., Sangwan-Norreel, B. S. 1983: Modifications ultrastructurales provoquées par des traitements promoteurs de landrogenése chez le Datura innoxia Mill. Ann. Sci. Nat. Bot., 5, 97-114.

    'Modifications ultrastructurales provoquées par des traitements promoteurs de landrogenése chez le Datura innoxia Mill. ' () 5 Ann. Sci. Nat. Bot. : 97 -114.

    • Search Google Scholar
  • Dale, P. J. 1975: Pollen dimorphism and anther culture in barley. Planta, 127, 213-220.

    'Pollen dimorphism and anther culture in barley. ' () 127 Planta : 213 -220.

  • Dieu, P., Beckert, M. 1986: Further studies of androgenetic embryo production and plant regeneration from in vitro cultured anthers in maize (Zea mays L.). Maydica, 31, 245-259.

    'Further studies of androgenetic embryo production and plant regeneration from in vitro cultured anthers in maize (Zea mays L.). ' () 31 Maydica : 245 -259.

    • Search Google Scholar
  • Dunwell, J. M., Sunderland, N. 1974: Pollen ultrastructure in anther cultures of Nicotiana tabacum. II. Changes associated with embryogenesis. J. Exp. Bot., 25, 363-369.

    'Pollen ultrastructure in anther cultures of Nicotiana tabacum. II. Changes associated with embryogenesis. ' () 25 J. Exp. Bot. : 363 -369.

    • Search Google Scholar
  • Garrido, D., Vicente, O., Eller, N., Heberle-Bors, E., Rodriguez-García, M.I. 1993: De novo transcription of specific mRNAs during the induction of tobacco pollen embryogenesis. Sex. Plant Reprod., 6, 40-45.

    'De novo transcription of specific mRNAs during the induction of tobacco pollen embryogenesis. ' () 6 Sex. Plant Reprod. : 40 -45.

    • Search Google Scholar
  • Garrido, D., Eller, N., Heberle-Bors, E., Vicente, O. 1995: Cellular changes during the acquisition of embryogenic potential in isolated pollen grains of Nicotiana tabacum. Protoplasma, 186, 220-230.

    'Cellular changes during the acquisition of embryogenic potential in isolated pollen grains of ' () 186 Nicotiana tabacum. Protoplasma : 220 -230.

    • Search Google Scholar
  • Genovesi, A. D., Collins, G. B. 1982: In vitro production of haploid plants of corn via anther culture. Crop Sci., 22, 1137-1144.

    'In vitro production of haploid plants of corn via anther culture. ' () 22 Crop Sci. : 1137 -1144.

    • Search Google Scholar
  • Heberle-Bors, E. 1985: In vitro haploid formation from pollen, a critical review. Theor. Appl. Genet., 71, 361-374.

    'In vitro haploid formation from pollen, a critical review. ' () 71 Theor. Appl. Genet. : 361 -374.

    • Search Google Scholar
  • Horner, M., Pratt, M. L. 1979: Amino acid analysis of in vivo and androgenic anthers of Nicotiana tabacum. Protoplasma, 98, 279-282.

    'Amino acid analysis of in vivo and androgenic anthers of ' () 98 Nicotiana tabacum. Protoplasma : 279 -282.

    • Search Google Scholar
  • Horner, M., Street, E. 1978: Pollen dimorphism - Origin and significance in pollen plant formation by anther culture. Ann. Bot., 42, 763-777.

    'Pollen dimorphism - Origin and significance in pollen plant formation by anther culture. ' () 42 Ann. Bot. : 763 -777.

    • Search Google Scholar
  • Kyo, M., Harada, H. 1986: Control of the developmental pathway of tobacco pollen in vitro. Planta, 168, 427-432.

    'Control of the developmental pathway of tobacco pollen in vitro. ' () 168 Planta : 427 -432.

    • Search Google Scholar
  • Nakano, H., Maeda, E. 1989: Ultrastructure of inoculated anthers in relation to the frequency of induction of pollen callus in anther culture of Oryza sativa L. Japan. Jour. Crop Sci., 58, 204-211.

    'Ultrastructure of inoculated anthers in relation to the frequency of induction of pollen callus in anther culture of Oryza sativa L. ' () 58 Japan. Jour. Crop Sci. : 204 -211.

    • Search Google Scholar
  • Nitsch, C. 1974: La culture de pollen isolé sur milieu synthétique. C. R. Hebd. Sciences Acad. Sci., Ser. D., 278, 1031-1034.

    'La culture de pollen isolé sur milieu synthétique. ' () 278 C. R. Hebd. Sciences Acad. Sci., Ser. D. : 1031 -1034.

    • Search Google Scholar
  • Nitsch, C., Norreel, B. 1973: Effet dun choc thermique sur le pouvoir embryogéne du pollen de Datura innoxia cultivé dans lanthére et isolé de lanthére. C.R. Acad. Sci., Paris, 276, 303-306.

    'Effet dun choc thermique sur le pouvoir embryogéne du pollen de Datura innoxia cultivé dans lanthére et isolé de lanthére. ' () 276 C.R. Acad. Sci., Paris : 303 -306.

    • Search Google Scholar
  • Sangwan-Norreel, B. S. 1978: Cytochemical and ultrastructural peculiarities of embryogenic pollen grains and of young androgenic embryos in Datura innoxia. Can. J. Bot., 56, 805-817.

    'Cytochemical and ultrastructural peculiarities of embryogenic pollen grains and of young androgenic embryos in ' () 56 Datura innoxia. Can. J. Bot. : 805 -817.

    • Search Google Scholar
  • Sangwan, R. S., Camefort, H. 1983: The tonoplast, a specific marker of embryogenic microspores of Datura cultured in vitro. Histochemistry, 78, 473-480.

    'The tonoplast, a specific marker of embryogenic microspores of Datura cultured in vitro. ' () 78 Histochemistry : 473 -480.

    • Search Google Scholar
  • Sangwan, R. S., Sangwan-Norreel, B. S. 1987: Biochemical cytology of pollen embryogenesis. International Review of Cytology, 107. Pollen, Cytology and Development, pp. 221-226.

    'Biochemical cytology of pollen embryogenesis. ' () 107 International Review of Cytology : 221 -226.

    • Search Google Scholar
  • Taylor, A. O., Craig, A. S. 1971: Plants under climatic stress. II. Low temperature, high light effects on chloroplast ultrastructure. Plant Physiol., 47, 719-725.

    'Plants under climatic stress. II. Low temperature, high light effects on chloroplast ultrastructure. ' () 47 Plant Physiol. : 719 -725.

    • Search Google Scholar
  • Venable, J. H., Coggeshall,R. 1965: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 25, 407-408.

    'A simplified lead citrate stain for use in electron microscopy. ' () 25 J. Cell Biol. : 407 -408.

    • Search Google Scholar
  • Sunderland, N., Huang, B., Hills, G. J. 1984: Disposition of pollen in situ and its relevance to anther/pollen culture. J. Exp. Bot., 35, 521-530.

    'Disposition of pollen in situ and its relevance to anther/pollen culture. ' () 35 J. Exp. Bot. : 521 -530.

    • Search Google Scholar

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