Acta Agronomica Hungarica
Volume/Issue:
Volume 52: Issue 1
Evaluation of genetic stocks derived from Hordeum vulgare L. × H. bulbosum L. crosses
Authors:
A. Mihailescu Department of Wheat and Barley Cytogenetics, Agricultural Research and Development Institute Fundulea, Romania

Search for other papers by A. Mihailescu in
Current site
Google Scholar
PubMed Close
and
A. Giura Department of Wheat and Barley Cytogenetics, Agricultural Research and Development Institute Fundulea, Romania

Search for other papers by A. Giura in
Current site
Google Scholar
PubMed Close
Pages:
53–61
Online Publication Date:
22 Jul 2005
Publication Date:
01 Jun 2004
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.52.2004.1.7
Keywords:
barley DHLs; barley DHLs; barley DHLs; Hordeum bulbosum; triploids; cytogenetical selection; BC1
Restricted access

The aim of the programme started at ARDI-Fundulea in 1999 is to improve the pest and disease resistance of cultivated barley (H. vulgare L.) by introgressing valuable genes from the wild species H. bulbosum L. The paper presents results on the development and cytogenetical characterization of primary genetic stocks represented by diploid, triploid and tetraploid interspecific hybrids and first backcrossed generation descendants. several sterile diploid hybrids were found during the phenotypic screening and cytological analysis of haploid progeny from H. vulgare 2x × H. bulbosum 2x crosses. These hybrids were treated with colchicine and fertile tetraploid hybrids were obtained. Significant improvements in the seed setting and in vitro triploid hybrid regeneration were obtained using doubled haploid lines (DHLs), previously selected for high interspecific crossability, in crosses with a tetraploid cytotype of H. bulbosum. Meiosis analysis of triploid hybrids provided compelling evidence that relatively high intergenomic allosyndetic pairing had occurred in some of the triploids with increased potential for crossing over and genetic recombination. High mean values for hybrid stability, multivalent associations in MI, higher chiasma frequency per PMC and partial pollen fertility were considered by far the most important criteria in the cytogenetic selection of triploid hybrids. Selected triploids were backcrossed to barley DHLs. Among the in vitro regenerated backcrossed progeny several putative substitution lines (SLs) were identified by preliminary cytological screening. The complete phenotypic and cytogenetic characterization and disease resistance tests of tetraploid hybrids and putative SLs or RLs are now in progress.

  • Pickering, R. A. (1988): The production of fertile triploid hybrids between Hordeum vulgare L. (2n = 2x = 14) and H. bulbosum L. (2n = 4x = 28). Barley Genet. Newsl., 18, 25-29.

    'The production of fertile triploid hybrids between Hordeum vulgare L. (2n = 2x = 14) and H. bulbosum L. (2n = 4x = 28) ' () 18 Barley Genet. Newsl. : 25 -29 .

    • Search Google Scholar

  • Pickering, R. A. (1991): The production of fertile triploid hybrids from crosses between Hordeum vulgare L. (2n = 4x = 28) and H. bulbosum L. (2n = 2x = 14). Hereditas, 114, 227-236.

    'The production of fertile triploid hybrids from crosses between Hordeum vulgare L. (2n = 4x = 28) and H. bulbosum L. (2n = 2x = 14) ' () 114 Hereditas : 227 -236 .

    • Search Google Scholar

  • Pickering, R. A. (1992): Monosomic and double monosomic substitution of Hordeum bulbosum L. chromosomes into H. vulgare L. Theor. Appl. Genet., 84, 466-472.

    'Monosomic and double monosomic substitution of Hordeum bulbosum L. chromosomes into H. vulgare L ' () 84 Theor. Appl. Genet. : 466 -472 .

    • Search Google Scholar

  • Pickering, R. A., Johnston, P. A., Timmerman Vaughan, G. H., Cromey, M. B., Forbes, E. M., Steffenson., B. J., et al. (2000): Hordeum bulbosum - A new source of disease and pest resistance genes for use in barley breeding programmes. Barley Genet. Newsl., 30, 7-10.

    'Hordeum bulbosum - A new source of disease and pest resistance genes for use in barley breeding programmes ' () 30 Barley Genet. Newsl. : 7 -10 .

    • Search Google Scholar

  • Xu, J., Kasha, K. J. (1992): Transfer of a dominant gene for powdery mildew resistance and DNA from Hordeum bulbosum into cultivated barley (H. vulgare). Theor. Appl. Genet., 84, 771-777.

    'Transfer of a dominant gene for powdery mildew resistance and DNA from Hordeum bulbosum into cultivated barley ' () 84 (H. vulgare). Theor. Appl. Genet. : 771 -777 .

    • Search Google Scholar

  • Walther, U., Hebekus, A., Kopahnke, D., Proesler, G., Schliphake, E. (1999): Evaluation of IPK's barley germplasm for disease resistance. Rep. of Working Group on Barley of ECP/GR, Germany, pp. 93-103.

  • Ho, K. M., Kasha, K. J. (1975): Genetic control of chromosome elimination during haploid formation in barley. Genetics, 81, 263-275.

    'Genetic control of chromosome elimination during haploid formation in barley ' () 81 Genetics : 263 -275 .

    • Search Google Scholar

  • Lange, W. (1971): Crosses between Hordeum vulgare L. and Hordeum bulbosum L. 1. Production, morphology and meiosis of hybrids, haploids and dihaploids. Euphytica, 20, 14-29.

    'Crosses between Hordeum vulgare L. and Hordeum bulbosum L. 1. Production, morphology and meiosis of hybrids, haploids and dihaploids ' () 20 Euphytica : 14 -29 .

    • Search Google Scholar

  • Lehmann, L. C. (1991): The use of genetic resources for isolating disease resistance for barley cultivar development. In: Barley Genetics VI, pp. 650-652.

  • Mihãilescu, A., Giura, A. (1999a): Hybrid and triploid progenies from Hordeum vulgare L. (2 n = 14) x Hordeum bulbosum L. (2 n = 14 and 2 n = 28) interspecific crosses. In: COST 824, "Wide crosses in cereals - problems and applications". Krakow, Poland, pp. 21-22.

  • Mihãilescu, A., Giura, A. (1999b): Production of barley doubled haploid lines by Bulbosum system. In: Proc. 2nd Balkan Symp. on Field Crops, 16-20 June, 1998, Novi-Sad, Yugoslavia, pp. 287-290.

  • Mihãilescu, A., Giura A. (2000): Use of the wild species Hordeum bulbosum L. in Romanian barley breeding programmes. In: Proc. XVI EUCARPIA, Genetic Resources Section, Poland, pp. 343-347.

  • Xu, J., Snape, J. W. (1987): Crossabilities of barley varieties with diploid and tetraploid clones of Hordeum bulbosum. Barley Genet. Newsl., 17, 40-43.

    'Crossabilities of barley varieties with diploid and tetraploid clones of ' () 17 Hordeum bulbosum. Barley Genet. Newsl. : 40 -43 .

    • Search Google Scholar

  • Xu, J., Snape, J. W. (1988): The cytology of hybrids between H. vulgare and H. bulbosum revisited. Genome, 30, 486-494.

    'The cytology of hybrids between H. vulgare and H. bulbosum revisited ' () 30 Genome : 486 -494 .

    • Search Google Scholar

  • Zeller, F. J. (1998): Improving cultivated barley (Hordeum vulgare L.) by making use of the genetic potential of wild Hordeum species. Angewandte Botanik, 75, 162-167.

    'Improving cultivated barley (Hordeum vulgare L.) by making use of the genetic potential of wild Hordeum species ' () 75 Angewandte Botanik : 162 -167 .

    • Search Google Scholar

  • Bothmer, R. von, Jacobsen, N., Baden, C., Jorgensen, R. B., Linde-Laursen, I. (1995): An Ecogeographical Study of the Genus Hordeum. IPGRI, Rome, 129 pp.

  • Gilpin, M. J., Pickering, R. A., Fautrier, A. G., Mc Neil, D. L., Szigat, G., Hill, A. M., Kynast, R. G. (1997): Morphological and molecular analysis of androgenetic selfed and backcrossed plants produced from a Hordeum vulgare x Hordeum bulbosum hybrid. Plant Breed., 116, 505-510.

    'Morphological and molecular analysis of androgenetic selfed and backcrossed plants produced from a Hordeum vulgare x Hordeum bulbosum hybrid ' () 116 Plant Breed. : 505 -510 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2025 20 0 0
Feb 2025 17 0 0
Mar 2025 36 0 0
Apr 2025 11 0 0
May 2025 19 0 0
Jun 2025 14 0 0
Jul 2025 0 0 0