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  • 1 Department of Wheat and Barley Cytogenetics, Agricultural Research and Development Institute Fundulea, Romania
  • | 2 Department of Wheat and Barley Cytogenetics, Agricultural Research and Development Institute Fundulea, Romania
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The aim of the programme started at ARDI-Fundulea in 1999 is to improve the pest and disease resistance of cultivated barley (H. vulgare L.) by introgressing valuable genes from the wild species H. bulbosum L. The paper presents results on the development and cytogenetical characterization of primary genetic stocks represented by diploid, triploid and tetraploid interspecific hybrids and first backcrossed generation descendants. several sterile diploid hybrids were found during the phenotypic screening and cytological analysis of haploid progeny from H. vulgare 2x × H. bulbosum 2x crosses. These hybrids were treated with colchicine and fertile tetraploid hybrids were obtained. Significant improvements in the seed setting and in vitro triploid hybrid regeneration were obtained using doubled haploid lines (DHLs), previously selected for high interspecific crossability, in crosses with a tetraploid cytotype of H. bulbosum. Meiosis analysis of triploid hybrids provided compelling evidence that relatively high intergenomic allosyndetic pairing had occurred in some of the triploids with increased potential for crossing over and genetic recombination. High mean values for hybrid stability, multivalent associations in MI, higher chiasma frequency per PMC and partial pollen fertility were considered by far the most important criteria in the cytogenetic selection of triploid hybrids. Selected triploids were backcrossed to barley DHLs. Among the in vitro regenerated backcrossed progeny several putative substitution lines (SLs) were identified by preliminary cytological screening. The complete phenotypic and cytogenetic characterization and disease resistance tests of tetraploid hybrids and putative SLs or RLs are now in progress.

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Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation
1950
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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