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  • 1 Cytogenetics and Tissue Culture Laboratory, Department of Plant Breeding and Genetics, CSK Himachal Pradesh Agricultural University Palampur, India
  • | 2 Cytogenetics and Tissue Culture Laboratory, Department of Plant Breeding and Genetics, CSK Himachal Pradesh Agricultural University Palampur, India
  • | 3 Cytogenetics and Tissue Culture Laboratory, Department of Plant Breeding and Genetics, CSK Himachal Pradesh Agricultural University Palampur, India
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Comparisons between androgenesis and maize-mediated haploid production efficiencies were made in six F1 genotypes each of winter × spring wheat and triticale × wheat crosses. The haploid status of the plantlets obtained was confirmed through cytological examination of the root tips. Much higher embryo formation (15.2%), haploid induction (8.7%) and doubled haploid production (8.3%) were obtained in the winter × spring wheat F1s through the wheat × maize system than by androgenesis (3.1%, 3.2 and 2.7%, respectively). Three of the triticale × wheat F1 genotypes failed to respond to androgenesis, while no haploids were recovered through the wheat × maize system in any of the six triticale × wheat F1s. Genotypic specificity, low callus induction and albinism reduced the efficiency of androgenesis both in winter × spring wheat and triticale × wheat hybrids. In all, the wheat × maize system proved to be better for winter × spring wheat hybrids and androgenesis for triticale × wheat hybrids.

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