Microsatellite markers and automated fragment analysis techniques for efficient and precise hybrid identification and genetic purity testing in pepper (Capsicum annuum L.)

A. Gémes Juhász; A. Stágel; S. Ács; L. Zatykó; et al.

doi:10.1556/AAgr.54.2006.2.3

Acta Agronomica Hungarica

Volume/Issue:: Volume 54: Issue 2

Microsatellite markers and automated fragment analysis techniques for efficient and precise hybrid identification and genetic purity testing in pepper (Capsicum annuum L.)

Authors:

A. Gémes Juhász

A. Gémes Juhász Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár, Hungary

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A. Stágel

A. Stágel Vegetable Crops Research Institute (ZKI Rt.) Budapest, Hungary

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S. Ács

S. Ács Agricultural Biotechnology Center, Gödöllő, Hungary

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L. Zatykó

L. Zatykó Vegetable Crops Research Institute (ZKI Rt.) Budapest, Hungary

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et al.

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Pages:: 141–146

Online Publication Date:: 08 Aug 2006

Publication Date:: 01 Jun 2006

Article Category:: Research Article

DOI:: https://doi.org/10.1556/AAgr.54.2006.2.3

Keywords:: hybrid identification; purity testing; pepper; microsatellites

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As locus-specific co-dominant PCR-based markers that allow semi-automated, high-throughput investigation technologies, microsatellites are ideal tools for genotype identification. Eleven of a set of 114 microsatellite markers available at the Agricultural Biotechnology Center proved to be suitable to distinguish between the parents of at least one of nine sweet pepper hybrid combinations. Markers with the highest information capacity were found to be capable of distinguishing between the parents of four different hybrid combinations and exhibited up to four different alleles in 18 haplotypes.

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Acta Agronomica Hungarica

Print ISSN:: 0238-0161

Online ISSN:: 1588-2527

Acta Agronomica Hungarica
Language	English Russian German French
Size
Year of Foundation	1950
Publication Programme	ceased
Volumes per Year
Issues per Year
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0238-0161 (Print)
ISSN	1588-2527 (Online)