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  • 1 Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt
  • | 2 Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt
  • | 3 Department of Seed Technology, Horticulture Research Institute, ARC, Ministry of Agriculture, Cairo, Egypt
  • | 4 Department of Seed Technology, Horticulture Research Institute, ARC, Ministry of Agriculture, Cairo, Egypt
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Seeds of tomato (Lycopersicon esculentum Mill., variety Castle rock) were osmoprimed in polyethylene glycol 6000 (PEG; 20%) or K2HPO4 (200 mM) solution for 8 hours, 3 days or 7 days, while another group of seeds were left in water for the same periods. The GA3/ABA ratio was the most important hormone factor, which promoted germination in seeds soaked in H2O and led to improved germination performance. This ratio showed slight variations between hydroprimed and osmoprimed seeds after 8 hours, but afterwards, from 3 to 7 days, it was gradually increased in the osmoprimed seeds and was substantially elevated in seeds germinating in H2O. Changes in the concentrations of phenolic compounds suggested their possible role in germination silencing in the osmoprimed seeds, but at relatively low concentrations. Protein patterns showed no marked variations in hydroprimed and osmoprimed seeds after 8 hours, but different types were observed, particularly after 7 days. A comparison of the protein banding patterns of seeds after 1 day and 7 days in the osmoconditioning solutions (PEG or K2HPO4), H2O, GA3 or ABA showed certain treatment-specific protein bands, particularly in PEG and ABA solutions. Longitudinal sections of seeds (after 3 days) showed lysis of the micropylar endosperm and radicle protrusion in H2O or GA3, whereas in PEG or K2HPO4 solution the radicle expanded inside the seed and the micropylar endosperm was completely intact. In ABA solution, the whole endosperm was compact and the seed became extensively desiccated.

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation
1950
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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