Acta Agronomica Hungarica
Volume/Issue:
Volume 55: Issue 1
Pyrophosphate:fructose 6-phosphate 1-phosphotransferase is involved in the mobilization of sugar reserves in the taproots of cold- and drought-stressed carrot plants
Authors: G. Kovács 1 , S. Sorvari 2 , P. Scott 3 , and O. Toldi 1
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  • 1 Agricultural Biotechnology Center Gödöllő Hungary
  • 2 MTT Agrifood Research Finland Horticulture Piikkiö Finland
  • 3 University of Sussex School of Life Sciences Brighton UK
DOI:
https://doi.org/10.1556/AAgr.55.2007.1.8
Page Count:
71–82
Publication Date:
01 Mar 2007
Online Publication Date:
28 Mar 2007
Article Category:
Research Article
Restricted access

The purpose of this work was to further investigate the regulatory interplay between pyrophosphate:fructose 6-phosphate 1-phosphotransferase (PFP) and its positive effector, fructose 2,6-bisphosphate (Fru-2,6-P 2 ), in the storage organs of cold- and drought-stressed plants. Since there is no detectable cytoplasmic fructose-1,6-bisphosphatase (cytFBPase) activity in the taproots of carrot plants, PFP is the only enzyme that can replace its function when stored starch is converted to transportable sucrose. The working hypothesis was that PFP is likely to be involved in the mobilisation of energy reserves and might have a special role in storage organs such as carrot taproots upon stress. Both cold and drought stress resulted in a marked increase in the endogenous Fru-2,6-P 2 levels. It is suggested that the significant changes in photosynthate allocation are the direct results of the stimulation of PFP activity by elevated Fru-2,6-P 2 levels. PFP stimulated by Fru-2,6-P 2 operated in the gluconeogenic direction in the taproots of stressed carrot plants, whereas the glycolytic direction was dominant in the non-stressed controls. This suggests that the metabolic status determining the net activity of PFP depends on the physiological stress situation, making PFP an important sensor of environmental changes. The experimental data indicated that PFP is involved in the mobilisation of energy reserves during unfavourable environmental changes by promoting the re-synthesis of transportable sucrose in taproots.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527
Keywords:
PFP; fructose 2,6-bisphosphate; abiotic stress; glycolysis; gluconeogenesis; Daucus carota ; taproot

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