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  • 1 Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár Hungary
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A number of sporophytically induced microspores and embryo-like structures (ELS) were obtained from isolated microspore cultures of durum wheat ( Triticum turgidum L. cv. Martondur 1). Various pre-treatments were screened, involving spike treatment at 4°C for 2, 7 or 14 days; anther treatment in 0.4 M mannitol containing macroelements at 33°C for 3 days, and various combinations of these. The frequency of embryogenic (star-like) microspores and the number of ELS showed a very high positive correlation in the cultures. Starvation at high temperature was necessary to achieve a reasonable frequency of microspore embryogenesis. The best results were achieved when starvation at high temperature was combined with no or short (2-day) cold treatment (212±77 and 203±34 ELS/100 anthers, respectively). However, the ELS failed to regenerate; only a few of them produced poorly-developed albino shoots. The present work could be a promising starting point for the production of doubled haploid durum wheat plants in Hungary via isolated microspore culture.

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Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation
1950
Publication
Programme
ceased
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per Year
 
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per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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