Acta Agronomica Hungarica
Volume/Issue:
Volume 55: Issue 3
In vitro and in vivo screening for cold tolerance in winter × spring wheat-derived doubled haploids following the wheat × maize system
Authors:
S. Sharma CSK Himachal Pradesh Agricultural University Molecular Cytogenetics and Tissue Culture Laboratory, Department of Plant Breeding and Genetics Palampur India

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H. Chaudhary CSK Himachal Pradesh Agricultural University Molecular Cytogenetics and Tissue Culture Laboratory, Department of Plant Breeding and Genetics Palampur India

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Pages:
273–282
Online Publication Date:
12 Sep 2007
Publication Date:
01 Sep 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.55.2007.3.2
Keywords:
cold tolerance; in vitro ; in vivo ; doubled haploids; winter × spring wheat hybridization; wheat × maize system
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Seventy-eight doubled haploid (DH) lines, derived from 21 elite and diverse winter × spring wheat F 1 hybrids, following the wheat × maize system, were screened along with the parental genotypes under in vitro and in vivo conditions for cold tolerance. Under in vitro conditions, the 2,3,5-triphenyl tetrazolium chloride (TTC) test was used to characterize the genotypes for cold tolerance. Based on the TTC test, only one doubled haploid, DH 69, was characterized as cold-tolerant, seven DH and five winter wheat parents were moderately tolerant, while the rest were susceptible. Analysis of variance under in vivo conditions also indicated the presence of sufficient genetic variability among the genotypes (DH lines + parents) for all the yield-contributing traits under study. The correlation and path analysis studies underlined the importance of indirect selection for tillers per plant, harvest index and grains per spike in order to improve grain yield. It was also concluded that selection should not be practised for grain weight per spike as it would adversely affect the grain yield per plant. When comparing the field performance of the genotypes with the in vitro screening parameters, it was concluded that in addition to the TTC test, comprising a single parameter, other physiological and biochemical in vitro parameters should be identified, which clearly distinguish between cold-tolerant and susceptible genotypes and also correlate well with their performance under field conditions.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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