Acta Agronomica Hungarica
Volume/Issue:
Volume 55: Issue 3
Co-inoculation of nitrogen-fixing and phosphate-solubilizing bacteria to promote growth, yield and nutrient uptake in chickpea
Authors:
P. Wani Aligarh Muslim University Department of Agricultural Microbiology, Faculty of Agricultural Sciences Aligarh U. P. India

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M. Khan Aligarh Muslim University Department of Agricultural Microbiology, Faculty of Agricultural Sciences Aligarh U. P. India

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A. Zaidi Aligarh Muslim University Department of Agricultural Microbiology, Faculty of Agricultural Sciences Aligarh U. P. India

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Pages:
315–323
Online Publication Date:
12 Sep 2007
Publication Date:
01 Sep 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.55.2007.3.7
Keywords:
nitrogen-fixing organisms; phosphate-solubilizing bacteria; chickpea; N uptake
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A total of 32 bacterial isolates including Mesorhizobium (N=10), Azotobacter (N=12) and phosphate-solubilizing bacteria (N=10) were isolated and tested for siderophore, HCN, ammonia, indole acetic acid production and phosphate solubilization in vitro . The bacterial cultures were positive for siderophore, HCN and ammonia. Among the isolates, M. ciceri RC3 and A. chrococcum A4 displayed 35 and 14 μg ml −1 of IAA, respectively, whereas Bacillus produced 19 ( Bacillus PSB1) and 17 μg ml −1 ( Bacillus PSB10) of IAA in Luria Bertani broth. The diameter of the P solubilization zone varied between 4 ( Bacillus PSB1) and 5 mm ( Bacillus PSB10) and a considerable amount of tricalcium phosphate (7 and 8 μg ml −1 by Bacillus PSB1 and Bacillus PSB10, respectively) was released in liquid medium, with a concomitant drop in pH. The effects of N 2 -fixing and PS bacteria on the growth, chlorophyll content, seed yield, grain protein and N uptake of chickpea plants in field trials varied considerably between the treatments. Nodule number and biomass were significantly greater at 90 days after sowing (DAS), decreasing by 145 DAS. Seed yield increased by 250% due to inoculation with M. ciceri RC3 + A. chroococcum A4 + Bacillus PSB10, relative to the control treatment. Grain protein content ranged from 180 ( Bacillus PSB1) to 309 ng g −1 ( M. ciceri RC3 + A. chroococcum A4 + Bacillus PSB10) in inoculated chickpea. The N contents in roots and shoots differed considerably among the treatments.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
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French
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Year of
Foundation		 1950
Publication
Programme		 ceased
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per Year		  
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Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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