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  • 1 CCS Haryana Agricultural University Department of Plant Breeding Haryana India
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The present investigation was conducted to investigate the impact of bio-inoculants on the magnitude and direction of gene effects and mean performance for root length density, root biomass per plant, AMF colonization in roots and micronutrient uptake (Cu, Fe, Mn, Zn) in wheat under low input field conditions. The material for study comprised three wheat cultivars, WH 147 (low mineral input), WH 533 (drought-tolerant), Raj 3077 (high mineral input) and six generations (P 1 , P 2 , F 1 , F 2 , BC 1 and BC 2 ) of three crosses, namely WH 147 × WH 533, WH 533 × Raj 3077 and WH 147 × Raj 3077. The experiment was conducted in a randomized block design with three replications having three treatments, i.e. (i) control; (ii) inoculation with arbuscular mycorrhizal fungi (AMF, Glomus fasciculatum ); (iii) dual inoculation with AMF and Azotobacter chroococcum ( Azc ). The fertilizer doses in all three treatments were 80 kg N + 40 kg P + 18 kg ZnSO 4 ha −1 . Root length density, root biomass per plant, AMF colonization in roots and Zn and Mn content were found to be maximum after dual inoculation with AMF+ Azc in all three crosses. Joint scaling tests revealed that additive-dominance gene effects were mainly operative in governing the expression of root biomass, Cu and Zn content in all three crosses for all three treatments (i.e. control, AMF and AMF + Azc ). Pedigree selection in crosses WH 147 × WH 533 and WH 147 × Raj 3077 could be effective for breeding pure lines of wheat for sustainable agriculture (low input genotypes responsive to biofertilizers such as AMF and Azotobacter ).

  • Behl, R. K., Sharma, H., Kumar, V., Narula, N. (2003): Interactions amongst mycorrhiza, Azotobacter chroococcum and root characteristics of wheat varieties. J. Agron. Crop Sci. , 189 , 151–155.

    Narula N. , 'Interactions amongst mycorrhiza, Azotobacter chroococcum and root characteristics of wheat varieties ' (2003 ) 189 J. Agron. Crop Sci. : 151 -155.

    • Search Google Scholar
  • Cavalli, L. L. (1952): An analysis of linkage in quantitative inheritance. pp. 135–144. In: Reoxe, E. C. R., Weddington, C. H. (eds.), Quantitative Inheritance . HMSO, London.

    Cavalli L. L. , '', in Quantitative Inheritance , (1952 ) -.

  • Heinzemann, J. (1994): Production of glass-wool inoculum of arbuscular mycorrhizal fungi . Ph.D. dissertation in German, abstract in English. University of Göttingen, Germany.

    Heinzemann J. , '', in Production of glass-wool inoculum of arbuscular mycorrhizal fungi , (1994 ) -.

    • Search Google Scholar
  • Hetrick, B. A. D., Wilson, G. W. T., Todd, T. C. (1996): Mycorrhizal response in wheat cultivars: Relationship to phosphorus. Can. J. Bot. , 74 , 19–25.

    Todd T. C. , 'Mycorrhizal response in wheat cultivars: Relationship to phosphorus ' (1996 ) 74 Can. J. Bot. : 19 -25.

    • Search Google Scholar
  • Jensen, V., 1951. Notes on the biology of Azotobacter . Proceedings of the Society for Applied Bacteriology , 74 , pp. 93–98.

    Jensen V. , 'Notes on the biology of Azotobacter ' (1951 ) 74 Proceedings of the Society for Applied Bacteriology : 93 -98.

    • Search Google Scholar
  • Jinks, J. L., Jones, R. M. (1958): Estimation of the components of heterosis. Genetics , 43 , 223–234.

    Jones R. M. , 'Estimation of the components of heterosis ' (1958 ) 43 Genetics : 223 -234.

  • Manske, G. G. B., Vlek, P. L. G. (2002): Root Architecture — Wheat as a Model Plant. pp. 249–259. In: Waisel, Y., Eshel, A., Kafkafi, U. (eds.), Plant Roots: The Hidden Half . 3 rd Edition. Marcel Dekker Inc., New York, Basel.

    Vlek P. L. G. , '', in Plant Roots: The Hidden Half , (2002 ) -.

  • Manske, G. G. B., Luttger, A. B., Behl, R. K., Vlek, P. L. G. (1995): Nutrient efficiency based on VA mycorrhiza and total root length of wheat cultivars grown in India. Angew. Bot. , 69 , 108–110.

    Vlek P. L. G. , 'Nutrient efficiency based on VA mycorrhiza and total root length of wheat cultivars grown in India ' (1995 ) 69 Angew. Bot. : 108 -110.

    • Search Google Scholar
  • Narula, N., Deubel, A., Gransee, A., Behl, R. K., Merbach, W. (2002): Impact of fertilizers on total microbiological flora in planted and unplanted soils of long-term fertilization experiment. Arch. Agr. Soil Sci. , 48 , 171–180.

    Merbach W. , 'Impact of fertilizers on total microbiological flora in planted and unplanted soils of long-term fertilization experiment ' (2002 ) 48 Arch. Agr. Soil Sci. : 171 -180.

    • Search Google Scholar
  • Phillips, I. M., Hayman, D. S. (1970): Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. , 55 , 158–161.

    Hayman D. S. , 'Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection ' (1970 ) 55 Trans. Br. Mycol. Soc. : 158 -161.

    • Search Google Scholar
  • Tennant, D. (1975): A test of modified line intersects method of estimating root length. J. Ecol. , 63 , 995–1001.

    Tennant D. , 'A test of modified line intersects method of estimating root length ' (1975 ) 63 J. Ecol. : 995 -1001.

    • Search Google Scholar