View More View Less
  • 1 Bulgarian Academy of Sciences Acad. M. Popov Institute of Plant Physiology Sofia Bulgaria
Restricted access

The response of the antioxidant enzymes peroxidase [EC 1.11.1.11], superoxide dismutase (SOD) [EC 1.15.1.1] and catalase [EC 1.11.1.6] to dehydration stress caused by low and high temperature, salinity (0.2 M NaCl) and hyperosmoticum (0.5 M sucrose), as well as to exogenous ABA and H 2 O 2 , was examined in germinating wheat seeds. The data presented here confirm and complete previous results for other stages of wheat seedling development (Bakalova et al., 2004; 2007). Catalase was the most susceptible antioxidant enzyme under the chronic stress conditions applied. Its activity correlated closely to the decrease in the growth rate of wheat seedlings. Low temperature had the strongest effect of all the stress factors applied. There was a significant decrease in anionic peroxidase activity, accompanied by catalase inhibition, after low temperature treatment. An analysis of all the data obtained revealed that the treatments had mostly non-specific effects on gene expression, protein and enzyme profiles. Catalase and peroxidase activity were suppressed not only by low temperature, but by hyperosmoticum (0.5 M sucrose) as well. This result confirmed findings that a significant number of genes induced by one particular stress are also upregulated by other stresses (Kreps et al., 2002; Munns, 2002; Rabbani et al., 2003).

  • Alscher, R. G., Erturk, N., Heath, L. S. (2002): Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. , 53 , 1331–1341.

    Heath L. S. , 'Role of superoxide dismutases (SODs) in controlling oxidative stress in plants ' (2002 ) 53 J. Exp. Bot. : 1331 -1341.

    • Search Google Scholar
  • Anderson, M. D., Prassad, T. K., Stewart, C. R. (1995): Changes in isozyme profiles of catalase, peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings. Plant Physiol. , 109 , 1247–1257.

    Stewart C. R. , 'Changes in isozyme profiles of catalase, peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings ' (1995 ) 109 Plant Physiol. : 1247 -1257.

    • Search Google Scholar
  • Azavedo-Neto, A. D., Prisco, J. T., Eneas-Filho, J., Medeiros, J. V. R., Gomes-Filho, E. (2005): Hydrogen peroxide pretreatment induces salt-stress acclimation in maize plants. J. Plant Physiol. , 162 , 1114–1122.

    Gomes-Filho E. , 'Hydrogen peroxide pretreatment induces salt-stress acclimation in maize plants ' (2005 ) 162 J. Plant Physiol. : 1114 -1122.

    • Search Google Scholar
  • Baek, K-H., Skinner, D. J. (2003): Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines. Plant Sci. , 165 , 1221–1227.

    Skinner D. J. , 'Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines ' (2003 ) 165 Plant Sci. : 1221 -1227.

    • Search Google Scholar
  • Bailly, C. (2004): Active oxygen species and antioxidants in seed biology. Seed Sci. Res. , 14 , 93–107.

    Bailly C. , 'Active oxygen species and antioxidants in seed biology ' (2004 ) 14 Seed Sci. Res. : 93 -107.

    • Search Google Scholar
  • Bailly, C., Benamar, A., Corbinaeu, F., Côme, D. (1998): Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol. Plant. , 104 , 646–652.

    Côme D. , 'Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds ' (1998 ) 104 Physiol. Plant. : 646 -652.

    • Search Google Scholar
  • Bailly, C., Leymarie, C. J., Lehner, A., Rousseau, S., Côme, D., Corbineau, F. (2004): Catalase activity and expression in developing sunflower seeds as related to drying. J. Exp. Bot. , 55 , 475–483.

    Corbineau F. , 'Catalase activity and expression in developing sunflower seeds as related to drying ' (2004 ) 55 J. Exp. Bot. : 475 -483.

    • Search Google Scholar
  • Bakalova, S., Nikolova, A., Nedeva, D. (2004): Isoenzyme profiles of peroxidase, catalase and superoxide dismutase as affected by dehydration stress and ABA during germination of wheat seeds. Bulg. J. Plant Physiol. , 30 , 64–77.

    Nedeva D. , 'Isoenzyme profiles of peroxidase, catalase and superoxide dismutase as affected by dehydration stress and ABA during germination of wheat seeds ' (2004 ) 30 Bulg. J. Plant Physiol. : 64 -77.

    • Search Google Scholar
  • Bakalova, S., Nedeva, D., McKee, J. (2007): Protein profiles in wheat seedlings subjected to dehydration stress. Appl. Ecol. Environ. Res. (in press).

  • Bewley, J. D., Black, M. (1994): In: Seeds: Physiology of Development and Germination (2nd edition). Plenum Press, New York and London.

    Black M. , '', in Seeds: Physiology of Development and Germination , (1994 ) -.

  • Bohnert, H., Nelson, D. E., Jensen, R. G. (1995): Adaptations to environmental stresses. Plant Cell , 7 , 1099–1111.

    Jensen R. G. , 'Adaptations to environmental stresses ' (1995 ) 7 Plant Cell : 1099 -1111.

  • Davis, B. J. (1964): Disc Electrophoresis. II: Method and Application to Human Serum Proteins. Ann. N. Y. Acad. Sci. , 15 , 404–427.

    Davis B. J. , 'Disc Electrophoresis. II: Method and Application to Human Serum Proteins ' (1964 ) 15 Ann. N. Y. Acad. Sci. : 404 -427.

    • Search Google Scholar
  • Donahue, J. L., Okpodu, C. M., Kramer, C. L., Grabau, E. A., Alscher, R. G. (1997): Responses of antioxidants to paraquat in pea leaves. Relationships to resistance. Plant Physiol. , 113 , 249–257.

    Alscher R. G. , 'Responses of antioxidants to paraquat in pea leaves. Relationships to resistance ' (1997 ) 113 Plant Physiol. : 249 -257.

    • Search Google Scholar
  • Farrant, J. M., Bailly, C., Leymarie, J., Hamman, B., Côme, D., Corbineau, F. (2004): Wheat seedlings as a model to understand desiccation tolerance and sensitivity. Physiol. Plant. , 120 , 563–574.

    Corbineau F. , 'Wheat seedlings as a model to understand desiccation tolerance and sensitivity ' (2004 ) 120 Physiol. Plant. : 563 -574.

    • Search Google Scholar
  • Foyer, C. H., Descourvieres, P., Kunert, K. J. (1994): Protection against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant Cell Environ. , 17 , 507–523.

    Kunert K. J. , 'Protection against oxygen radicals: an important defense mechanism studied in transgenic plants ' (1994 ) 17 Plant Cell Environ. : 507 -523.

    • Search Google Scholar
  • Foyer, C. H., Noctor, G. (2005): Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ. , 28 , 1056–1071.

    Noctor G. , 'Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context ' (2005 ) 28 Plant Cell Environ. : 1056 -1071.

    • Search Google Scholar
  • Gadjev, I., Vanderauwera, S., Gechev, T. S., Laloi, C., Minkov, I. N., Shulaev, V., Apel, K., Inze, D., Mittler, R., Van Breusegem, F. (2006): Transcriptomic footprints disclose specificity of reactive oxygen species signalling in Arabidopsis . Plant Physiol. , 141 , 436–445.

    Breusegem F. , 'Transcriptomic footprints disclose specificity of reactive oxygen species signalling in Arabidopsis ' (2006 ) 141 Plant Physiol. : 436 -445.

    • Search Google Scholar
  • Gapper, C., Dolan, L. (2006): Control of plant development by reactive oxygen species. Plant Physiol. , 141 , 341–345.

    Dolan L. , 'Control of plant development by reactive oxygen species ' (2006 ) 141 Plant Physiol. : 341 -345.

    • Search Google Scholar
  • Gaspar, T., Penel, C., Castilo, J. F., Greppin, H. (1985): A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiol. Plant. , 64 , 418–423.

    Greppin H. , 'A two-step control of basic and acidic peroxidases and its significance for growth and development ' (1985 ) 64 Physiol. Plant. : 418 -423.

    • Search Google Scholar
  • Gechev, T., Gadjev, I., Van Breusegem, F., Inze, D., Dukiandjiev, S., Toneva, V., Minkov, I. (2002): Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of oxidative enzymes. Cell Mol. Life Sci. , 59 , 708–714.

    Minkov I. , 'Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of oxidative enzymes ' (2002 ) 59 Cell Mol. Life Sci. : 708 -714.

    • Search Google Scholar
  • Greneche, M., Lallemand, J., Michaud, O. (1991): Comparison of different enzyme loci as a means of distinguishing ryegrass varieties by electrophoresis. Seed Sci. Technol. , 19 , 147–158.

    Michaud O. , 'Comparison of different enzyme loci as a means of distinguishing ryegrass varieties by electrophoresis ' (1991 ) 19 Seed Sci. Technol. : 147 -158.

    • Search Google Scholar
  • Guan, L., Scandalios, J. G. (1998): Effects of the plant growth regulator abscisic acid and high osmoticum on the developmental expression of the maize catalase genes. Physiol. Plant. , 104 , 413–422.

    Scandalios J. G. , 'Effects of the plant growth regulator abscisic acid and high osmoticum on the developmental expression of the maize catalase genes ' (1998 ) 104 Physiol. Plant. : 413 -422.

    • Search Google Scholar
  • Halliwell, B. (2006): Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol. , 141 , 312–322.

    Halliwell B. , 'Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life ' (2006 ) 141 Plant Physiol. : 312 -322.

    • Search Google Scholar
  • Heath, R. L., Packer, L. (1968): Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochem. Biophys. , 125 , 189–198.

    Packer L. , 'Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation ' (1968 ) 125 Archives of Biochem. Biophys. : 189 -198.

    • Search Google Scholar
  • Herner, R. C. (1990): The effects of chilling temperatures during seed germination and early seedling growth. pp. 51–69. In: Wang, C. Y. (ed.), Chilling Injury of Horticultural Crops . CRC Press, Boca Raton.

    Herner R. C. , '', in Chilling Injury of Horticultural Crops , (1990 ) -.

  • Janda, T., Szalai, G., Rios-Gonzales, R., Veisz, O., Paldi, E. (2003): Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci. , 164 , 301–306.

    Paldi E. , 'Comparative study of frost tolerance and antioxidant activity in cereals ' (2003 ) 164 Plant Sci. : 301 -306.

    • Search Google Scholar
  • Jessup, W., Dean, R., Gebicki, J. (1994): Iodometric determination of hydroperoxides in lipids and proteins. Methods Enzymol. , 233 , 289–303.

    Gebicki J. , 'Iodometric determination of hydroperoxides in lipids and proteins ' (1994 ) 233 Methods Enzymol. : 289 -303.

    • Search Google Scholar
  • Kreps, J. A., Wu, Y., Chang, H-S., Zhu, T., Wang, X., Harper, J. F. (2002): Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol. , 130 , 2129–2141.

    Harper J. F. , 'Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress ' (2002 ) 130 Plant Physiol. : 2129 -2141.

    • Search Google Scholar
  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randal, R. L. (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem. , 193 , 265–275.

    Randal R. L. , 'Protein measurement with the Folin phenol reagent ' (1951 ) 193 J. Biol. Chem. : 265 -275.

    • Search Google Scholar
  • Mandhania, S., Madan, S., Sawhney, V. (2006): Antioxidant defense mechanism under stress in wheat seedlings. Biol. Plant. , 50 , 227–231.

    Sawhney V. , 'Antioxidant defense mechanism under stress in wheat seedlings ' (2006 ) 50 Biol. Plant. : 227 -231.

    • Search Google Scholar
  • Mauro, F., Van Eycken, N., Challou, P., Lucas, M., L’Oiseau, M. (2005): Characterization of new maize chloroplastic copper/zinc superoxide dismutase isoforms by high resolution native two-dimensional polyacrylamide gel electrophoresis. Identification of chilling responsive chloroplastic superoxide dismutase isoforms. Physiol. Plant. , 124 , 323–335

    L’Oiseau M. , 'Characterization of new maize chloroplastic copper/zinc superoxide dismutase isoforms by high resolution native two-dimensional polyacrylamide gel electrophoresis. Identification of chilling responsive chloroplastic superoxide dismutase isoforms ' (2005 ) 124 Physiol. Plant. : 323 -335.

    • Search Google Scholar
  • Munns, R. (2002): Comparative physiology of salt and water stress. Plant Cell Environ. , 25 , 239–250.

    Munns R. , 'Comparative physiology of salt and water stress ' (2002 ) 25 Plant Cell Environ. : 239 -250.

    • Search Google Scholar
  • Oidaira, H., Sano, S., Koshiba, T., Ushimaru, T. (2000): Enhancement of antioxidative enzyme activities in chilled rice seedlings. J. Plant Physiol. , 156 , 811–813.

    Ushimaru T. , 'Enhancement of antioxidative enzyme activities in chilled rice seedlings ' (2000 ) 156 J. Plant Physiol. : 811 -813.

    • Search Google Scholar
  • Ornstein, L. (1964): Enzyme Bulletin . Canalco Industrial Corporation. Rockville, Maryland, 12.

    Ornstein L. , '', in Enzyme Bulletin , (1964 ) -.

  • Peyrano, G., Talisnik, E., Quiroga, M., De Forchetti, S. M., Tigier, H. (1997): Salinity effects on hydraulic conductance, lignin content and peroxidase activity in tomato roots. Plant Physiol. Biochem. , 35 , 387–393.

    Tigier H. , 'Salinity effects on hydraulic conductance, lignin content and peroxidase activity in tomato roots ' (1997 ) 35 Plant Physiol. Biochem. : 387 -393.

    • Search Google Scholar
  • Pinhero, R. G., Rao, M. V., Paliyath, C., Murr, D. P., Fletcher, R. A. (1997): Changes in the activities of antioxidant enzymes and their relationship to genetic and paclobutrazolinduced chilling tolerance of maize seedlings. Plant Physiol. , 114 , 695–704.

    Fletcher R. A. , 'Changes in the activities of antioxidant enzymes and their relationship to genetic and paclobutrazolinduced chilling tolerance of maize seedlings ' (1997 ) 114 Plant Physiol. : 695 -704.

    • Search Google Scholar
  • Prasad, T. K. (1996): Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings: changes in antioxidant system, oxidation of proteins and lipids, and protease activities. Plant J. , 10 , 1017–1026.

    Prasad T. K. , 'Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings: changes in antioxidant system, oxidation of proteins and lipids, and protease activities ' (1996 ) 10 Plant J. : 1017 -1026.

    • Search Google Scholar
  • Rao, M. V., Palyath, G., Ormrod, D. P. (1996): Ultraviolet-B and ozone induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana . Plant Physiol. , 110 , 125–136.

    Ormrod D. P. , 'Ultraviolet-B and ozone induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana ' (1996 ) 110 Plant Physiol. : 125 -136.

    • Search Google Scholar
  • Rabbani, M. A., Maruyama, K., Abe, H., Khan, M. A., Katsura, K., Ito, Y., Yoshiwara, K., Seki, M., Shinozaki, K., Yamaguchi-Shinozaki, K. (2003): Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol. , 133 , 1755–1767.

    Yamaguchi-Shinozaki K. , 'Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses ' (2003 ) 133 Plant Physiol. : 1755 -1767.

    • Search Google Scholar
  • Schafer, L., Feierabend, J. (2000): Photoinactivation and protection of glycolate oxidase in vitro and in leaves. Z. Naturforsch. , 55 , 362–372.

    Feierabend J. , 'Photoinactivation and protection of glycolate oxidase in vitro and in leaves ' (2000 ) 55 Z. Naturforsch. : 362 -372.

    • Search Google Scholar
  • Srivalli, B., Sharma, G., Khanna-Chopra, R. (2003): Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery. Physiol. Plant. , 119 , 503–512.

    Khanna-Chopra R. , 'Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery ' (2003 ) 119 Physiol. Plant. : 503 -512.

    • Search Google Scholar
  • Suzuki, N., Mittler, R. (2006): Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction. Physiol. Plant. , 126 , 45–51.

    Mittler R. , 'Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction ' (2006 ) 126 Physiol. Plant. : 45 -51.

    • Search Google Scholar
  • Tanida, M. (1996): Catalase activity of rice seed embryo and its relation to germination rate at a low temperature. Breeding Sci. , 46 , 23–27.

    Tanida M. , 'Catalase activity of rice seed embryo and its relation to germination rate at a low temperature ' (1996 ) 46 Breeding Sci. : 23 -27.

    • Search Google Scholar
  • Vranova, E., Inze, D., Van Breusegem, F. (2002): Signal transduction during oxidative stress. J. Exp. Bot. , 53 , 1227–1236.

    Breusegem F. , 'Signal transduction during oxidative stress ' (2002 ) 53 J. Exp. Bot. : 1227 -1236.

    • Search Google Scholar
  • Wahid, A., Perveen, M., Gelani, S., Basra, S. M. A. (2007): Pretreatment of seed with H 2 O 2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J. Plant Physiol. , 164 , 283–294.

    Basra S. M. A. , 'Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins ' (2007 ) 164 J. Plant Physiol. : 283 -294.

    • Search Google Scholar
  • Willekens, H., Chamnongpol, S., Davey, M., Shraudner, M., Langebaertels, C., Van Montagu, M., Inzé, D., Van Camp, W. (1997): Catalase is a sink for H 2 O 2 and is indispensable for stress defence in C 3 plants. EMBO J. , 14 , 4806–4816.

    Camp W. , 'Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants ' (1997 ) 14 EMBO J. : 4806 -4816.

    • Search Google Scholar
  • Wojtyla, L., Garnczarska, M., Zalewski, T., Bednarski, W., Ratajczak, L., Jurga, S. (2006): A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds. J. Plant Physiol. , 163 , 1207–1220.

    Jurga S. , 'A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds ' (2006 ) 163 J. Plant Physiol. : 1207 -1220.

    • Search Google Scholar
  • Woodbury, W., Spenser, A. K., Stahmann, M. A. (1971): An improved procedure using ferricyanide for detecting catalase isoenzymes. Anal. Biochem. , 44 , 301–305.

    Stahmann M. A. , 'An improved procedure using ferricyanide for detecting catalase isoenzymes ' (1971 ) 44 Anal. Biochem. : 301 -305.

    • Search Google Scholar
  • Yu, Q., Rengel, Z. (1999): Drought and salinity differentially influence activities of superoxide dismutases in narrow-leafed lupins. Plant Sci. , 141 , 1–11.

    Rengel Z. , 'Drought and salinity differentially influence activities of superoxide dismutases in narrow-leafed lupins ' (1999 ) 141 Plant Sci. : 1 -11.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jul 2020 12 0 0
Aug 2020 1 0 0
Sep 2020 2 0 0
Oct 2020 0 0 0
Nov 2020 1 5 0
Dec 2020 1 0 0
Jan 2021 1 0 0