Acta Agronomica Hungarica
Volume/Issue:
Volume 56: Issue 2
Antioxidant enzymes in germinating wheat seeds as affected by dehydration stress, ABA and hydrogen peroxide
Authors: S. Sabeva 1 and D. Nedeva 1
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  • 1 Bulgarian Academy of Sciences Acad. M. Popov Institute of Plant Physiology Sofia Bulgaria
DOI:
https://doi.org/10.1556/AAgr.56.2008.2.1
Page Count:
113–127
Publication Date:
01 Jun 2008
Online Publication Date:
16 Aug 2008
Article Category:
Research Article
Restricted access

The response of the antioxidant enzymes peroxidase [EC 1.11.1.11], superoxide dismutase (SOD) [EC 1.15.1.1] and catalase [EC 1.11.1.6] to dehydration stress caused by low and high temperature, salinity (0.2 M NaCl) and hyperosmoticum (0.5 M sucrose), as well as to exogenous ABA and H 2 O 2 , was examined in germinating wheat seeds. The data presented here confirm and complete previous results for other stages of wheat seedling development (Bakalova et al., 2004; 2007). Catalase was the most susceptible antioxidant enzyme under the chronic stress conditions applied. Its activity correlated closely to the decrease in the growth rate of wheat seedlings. Low temperature had the strongest effect of all the stress factors applied. There was a significant decrease in anionic peroxidase activity, accompanied by catalase inhibition, after low temperature treatment. An analysis of all the data obtained revealed that the treatments had mostly non-specific effects on gene expression, protein and enzyme profiles. Catalase and peroxidase activity were suppressed not only by low temperature, but by hyperosmoticum (0.5 M sucrose) as well. This result confirmed findings that a significant number of genes induced by one particular stress are also upregulated by other stresses (Kreps et al., 2002; Munns, 2002; Rabbani et al., 2003).

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527
Keywords:
catalase; peroxidase; SOD; germination; dehydration stress; wheat

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