Author: Z. Szigeti 1
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  • 1 Eötvös Loránd University Department of Plant Physiology and Molecular Plant Biology, Institute of Biology Budapest Hungary
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The fluorescence imaging technique was elaborated primarily for the detection of the fluorescence traits accompanying changes in the physiological status of stressed plants. The paper summarises the conditions and technical background required for the use of multi-wavelength fluorescence imaging. Images of leaves were recorded at wavelengths of 440, 520, 690 and 740 nm. Possible applications are illustrated by studies on the leaves of stressed plants. An evaluation of the images is presented, including the necessary corrections and fluorescence ratios, examples of comparisons between imaging and functional activity measurements, and an evaluation of the diagnostic importance and reliability of imaging in detecting the effects of stressors in plants. The results demonstrate that the multi-wavelength fluorescence imaging of leaves is a useful method for detecting the presence of stress in plants and for determining the extent of the stress.

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