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  • 1 Bulgarian Academy of Sciences Acad. M. Popov Institute of Plant Physiology Sofia Bulgaria
  • 2 Institute of Experimental Botany Cas Prague Czech Republic
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Cytokinin oxidase/dehydrogenase (CKX: EC 1.5.99.12) is able to provide a means for the rapid turnover of its substrate and it has been considered responsible for changes in the cytokinin pool in an adverse environment. Mild temperature stresses (10°C and 33°C average) were applied to young pea plants of two varieties (cvs. Manuela and Scinado) in order to assess the response of the cytokinin pool and CKX activity to altered growth conditions. Both temperature treatments increased the isopentenyl adenine (iP) and isopentenyl adenine riboside (iPR) contents in stressed plants. This trend was far more pronounced in the leaves. Low temperature additionally resulted in elevated cis zeatin riboside ( cis ZR) and CKX activity. Heat did not influence the enzymatic activity in the leaves, while opposing trends were observed in the root-derived CKX activity of the two tested varieties. The data suggest that variance in the temperature provokes adaptive reactions in the cytokinin pool, which is maintained by CKX activity.

  • Atanasova, L., Dobrev, P., Pissarska, M., Malbeck, J., Pulcheva, D., Iliev, L. (2005): Effect of atrazine on major endogenous cytokinin types in maize plants. Comp. Rend. Acad. Bulg. Sci. , 58 , 1225–1228.

    Iliev L. , 'Effect of atrazine on major endogenous cytokinin types in maize plants ' (2005 ) 58 Comp. Rend. Acad. Bulg. Sci. : 1225 -1228.

    • Search Google Scholar
  • Atanasova, L. Y., Pissarska, M. G., Popov, G. S., Georgiev, G. I. (2004): Growth and endogenous cytokinins of juniper shoots as affected by high metal concentrations. Biol. Plant. , 48 , 157–159.

    Georgiev G. I. , 'Growth and endogenous cytokinins of juniper shoots as affected by high metal concentrations ' (2004 ) 48 Biol. Plant. : 157 -159.

    • Search Google Scholar
  • Bieleski, R. L. (1964): The problem of halting enzyme action when extracting plant tissues. Anal. Biochem. , 9 , 431–442.

    Bieleski R. L. , 'The problem of halting enzyme action when extracting plant tissues ' (1964 ) 9 Anal. Biochem. : 431 -442.

    • Search Google Scholar
  • Bradford, M. M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. , 72 , 248–254.

    Bradford M. M. , 'A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding ' (1976 ) 72 Anal. Biochem. : 248 -254.

    • Search Google Scholar
  • Brugière, N., Jiao, S., Hantke, S., Zinselmeier, C., Roessler, J. A., Niu, X., Jones, R. J., Habben, J. E. (2003): Cytokinin oxidase gene expression in maize is localized to the vasculature and is induced by cytokinins, abscisic acid and abiotic stress. Plant Physiol. , 132 , 1228–1240.

    Habben J. E. , 'Cytokinin oxidase gene expression in maize is localized to the vasculature and is induced by cytokinins, abscisic acid and abiotic stress ' (2003 ) 132 Plant Physiol. : 1228 -1240.

    • Search Google Scholar
  • Buchanan-Wollaston, V., Earl, S., Harrison, E., Mathas, E., Navapour, S., Page, T., Pink, D. (2003): The molecular analysis of leaf senescence — a genomic approach. Plant Biotech. J. , 1 , 3–22.

    Pink D. , 'The molecular analysis of leaf senescence — a genomic approach ' (2003 ) 1 Plant Biotech. J. : 3 -22.

    • Search Google Scholar
  • Cheikh, N. C., Jones, R. J. (1994): Disruption of maize kernel growth and development by heat stress: role of cytokinin/ABA balance. Plant Physiol. , 106 , 45–51.

    Jones R. J. , 'Disruption of maize kernel growth and development by heat stress: role of cytokinin/ABA balance ' (1994 ) 106 Plant Physiol. : 45 -51.

    • Search Google Scholar
  • Galuszka, P., Frébort, I., Šebela, M., Sauer, P., Jacobsen, S., Peč, P. (2001): Cytokinin oxidase or dehydrogenase? Eur. J. Biochem. , 268 , 450–461.

    Peč P. , 'Cytokinin oxidase or dehydrogenase? ' (2001 ) 268 Eur. J. Biochem. : 450 -461.

  • Galuszka, P., Frébortová, J., Luhová, L., Bilyeu, K., English, J., Frébort, I. (2005): Tissue localization of cytokinin dehydrogenase in maize: possible involvement of quinone species generated from plant phenolics by other enzymatic systems in the catalytic reaction. Plant Cell Physiol. , 46 , 716–728.

    Frébort I. , 'Tissue localization of cytokinin dehydrogenase in maize: possible involvement of quinone species generated from plant phenolics by other enzymatic systems in the catalytic reaction ' (2005 ) 46 Plant Cell Physiol. : 716 -728.

    • Search Google Scholar
  • Goicoechea, N., Antolin, M. C., Strnad, M., Sanchez-Diaz, M. (1996): Root cytokinins, acid phosphatase and nodule activity in drought-stressed mycorrhizal or nitrogen-fixing alfalfa plants. J. Exp. Bot. , 47 , 683–686.

    Sanchez-Diaz M. , 'Root cytokinins, acid phosphatase and nodule activity in drought-stressed mycorrhizal or nitrogen-fixing alfalfa plants ' (1996 ) 47 J. Exp. Bot. : 683 -686.

    • Search Google Scholar
  • Goicoechea, N., Dolézal, K., Antolin, M. C., Strnad, M., Sanchez-Diaz, M. (1995): Influence of mycorrhizae and Rhizobium on cytokinin content in drought-stressed alfalfa. J. Exp. Bot. , 46 , 1543–1549.

  • Hare, P. D., Cress, W. A., van Staden, J. (1997): The involvement of cytokinins in plant responses to environmental stress. Plant Growth Regul. , 23 , 79–103.

    Staden J. , 'The involvement of cytokinins in plant responses to environmental stress ' (1997 ) 23 Plant Growth Regul. : 79 -103.

    • Search Google Scholar
  • Huang, B. (2004): Recent advances in drought and heat stress physiology of turfgrass (a review). Acta Horticult. , 661 , 185–192.

    Huang B. , 'Recent advances in drought and heat stress physiology of turfgrass (a review) ' (2004 ) 661 Acta Horticult. : 185 -192.

    • Search Google Scholar
  • Kaminek, M., Motyka, V., Vankova, R. (1997): Regulation of cytokinin content in plant cells. Physiol. Plant. , 101 , 689–700.

    Vankova R. , 'Regulation of cytokinin content in plant cells ' (1997 ) 101 Physiol. Plant. : 689 -700.

    • Search Google Scholar
  • Lexa, M., Genkov, T., Malbeck, J., Machackova, I., Brzobohaty, B. (2003): Dynamics of endogenous cytokinin pools in tobacco seedlings: a modeling approach. Ann. Bot. , 91 , 585–597.

    Brzobohaty B. , 'Dynamics of endogenous cytokinin pools in tobacco seedlings: a modeling approach ' (2003 ) 91 Ann. Bot. : 585 -597.

    • Search Google Scholar
  • Li, R., Sosa, J., del Barrio, M. A., Zavala, M. E. (2000): Accumulation of cytokinin oxidase in Zea . mays under cold stress. Proc. Am. Soc. Plant Physiol. , 1 , 107–108.

    Zavala M. E. , 'Accumulation of cytokinin oxidase in Zea. mays under cold stress ' (2000 ) 1 Proc. Am. Soc. Plant Physiol. : 107 -108.

    • Search Google Scholar
  • Liberos-Minotta, C. A., Tipton, P. A. (1995): A colorimetric assay for cytokinin oxidase. Anal. Biochem. , 231 , 339–341.

    Tipton P. A. , 'A colorimetric assay for cytokinin oxidase ' (1995 ) 231 Anal. Biochem. : 339 -341.

    • Search Google Scholar
  • Liu, X., Huang, B., Banowetz, H. (2002): Cytokinin effects on creeping bentgrass responses to heat stress: I. Shoot and root growth. Crop Sci. , 42 , 457–465.

    Banowetz H. , 'Cytokinin effects on creeping bentgrass responses to heat stress: I. Shoot and root growth ' (2002 ) 42 Crop Sci. : 457 -465.

    • Search Google Scholar
  • Lur, H-S., Setter, T. L. (1993): Role of auxin in maize endosperm development. Timing of nuclear DNA endoreduplication, zein expression, and cytokinin. Plant Physiol. , 103 , 273–280.

    Setter T. L. , 'Role of auxin in maize endosperm development. Timing of nuclear DNA endoreduplication, zein expression, and cytokinin ' (1993 ) 103 Plant Physiol. : 273 -280.

    • Search Google Scholar
  • Manju, R. V., Kulkarni, M. J., Prasad, T. G., Sudashana, L., Sashidar, V. R. (2001): Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress. Indian J. of Exp. Biol. , 39 , 786–792.

    Sashidar V. R. , 'Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress ' (2001 ) 39 Indian J. of Exp. Biol. : 786 -792.

    • Search Google Scholar
  • Merzlyak, M., Hendry, G. (1994): Free radical metabolism, pigment degradation and lipid peroxidation in leaves during senescence. pp. 459–471. In: Crawford, R., Hendry, G., Goodman, B. (eds.), Oxygen and Environmental Stress in Plants . Proc. Royal Soc., 102B, Edinburgh

  • Mok, D. W. S., Mok, M. C. (2001): Cytokinin metabolism and action. Annu. Rev. Plant Physiol. Plant Mol. Biol. , 52 , 89–118.

    Mok M. C. , 'Cytokinin metabolism and action ' (2001 ) 52 Annu. Rev. Plant Physiol. Plant Mol. Biol. : 89 -118.

    • Search Google Scholar
  • Morris, R. O., Blevins, D. G., Dietrich, J. T., Durley, R. C., Gelvin, S. B., Gray, J., Hommes, N. G., Kaminek, M., Mathews, L. J., Meilan, B., Reinbott, T. M., Sayavedra-Soto, L. (1993): Cytokinins in plant pathogenic bacteria and developing cereal grains. Aust. J. Plant Physiol. , 20 , 621–637.

    Sayavedra-Soto L. , 'Cytokinins in plant pathogenic bacteria and developing cereal grains ' (1993 ) 20 Aust. J. Plant Physiol. : 621 -637.

    • Search Google Scholar
  • Pauls, K. P., Thompson, J. E. (1982): Effects of cytokinins and antioxidants on the susceptibility of membranes to ozone damage. Plant Cell Physiol. , 23 , 821–832.

    Thompson J. E. , 'Effects of cytokinins and antioxidants on the susceptibility of membranes to ozone damage ' (1982 ) 23 Plant Cell Physiol. : 821 -832.

    • Search Google Scholar
  • Tian, Q., Chen, F., Zhang, F., Mi, G. (2005): Possible involvement of cytokinin in nitrate mediated root growth in maize. Plant Soil , 277 , 185–196.

    Mi G. , 'Possible involvement of cytokinin in nitrate mediated root growth in maize ' (2005 ) 277 Plant Soil : 185 -196.

    • Search Google Scholar
  • Vaseva-Gemisheva, I., Lee, D., Karanov, E. (2005): Response of Pisum sativum cytokinin oxidase/dehydrogenase expression and specific activity to drought stress and herbicide treatments. Plant Growth Regul. , 46 , 199–208.

    Karanov E. , 'Response of Pisum sativum cytokinin oxidase/dehydrogenase expression and specific activity to drought stress and herbicide treatments ' (2005 ) 46 Plant Growth Regul. : 199 -208.

    • Search Google Scholar
  • Veselova, S. V., Farhutdinov, R. G., Veselov, S. Y., Kudoyarova, G. R., Veselov, D. S., Hartung, W. (2005): The effect of root cooling on hormone content, leaf conductance and root hydraulic conductivity of durum wheat seedlings ( Triticum durum L.). J. Plant Physiol. , 162 , 21–26.

    Hartung W. , 'The effect of root cooling on hormone content, leaf conductance and root hydraulic conductivity of durum wheat seedlings (Triticum durum L.) ' (2005 ) 162 J. Plant Physiol. : 21 -26.

    • Search Google Scholar
  • Warrington, I. J., Kanemasu, E. T. (1983): Corn growth response to temperature and photoperiod. I. Seedling emergence, tassel initiation, and anthesis. Agron. J. , 75 , 749–754.

    Kanemasu E. T. , 'Corn growth response to temperature and photoperiod. I. Seedling emergence, tassel initiation, and anthesis ' (1983 ) 75 Agron. J. : 749 -754.

    • Search Google Scholar
  • Werner, T., Motyka, V., Laucou, V., Smets, R., Van Onckelen, H., Schmulling, T. (2003): Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell , 15 , 2532–2550.

    Schmulling T. , 'Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity ' (2003 ) 15 Plant Cell : 2532 -2550.

    • Search Google Scholar

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