Acta Agronomica Hungarica
Volume/Issue:
Volume 57: Issue 2
Investigation of factors influencing the regeneration efficiency of Rubus species
Authors:
K. Kálai Agricultural Biotechnology Center Gödöllő Hungary

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,
M. Csányi Agricultural Biotechnology Center Gödöllő Hungary

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,
A. Mészáros Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár Hungary

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, and
E. Balázs Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár Hungary

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Pages:
149–154
Online Publication Date:
10 Jun 2009
Publication Date:
01 Jun 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.57.2009.2.6
Keywords:
Rubus ; in vitro regeneration; pre-treatment; cytokinins; stock plant maintenance
Restricted access

An efficient regeneration system is described based on the use of several genotypes and combining different cytokinins in the regeneration process. Optimal regeneration efficiency can be obtained if the factors affecting regeneration are examined with special attention to the maintenance of the stock plants, the composition of the medium, and the pre-treatment. The maintenance of stock plants proved to be optimal if the plants were kept on modified LS medium supplemented with 0.125 mg L −1 6-benzylaminopurine (BAP) and 0.01 mg L −1 indole-3-butyric acid (IBA) in large vessels. Pre-treatment was found to increase the regeneration efficiency. Placing the leaves on to medium containing 1.5 mg L −1 BAP-riboside and 0.1 mg L −1 thidiazuron (TDZ) without wounding, and keeping them in the dark for 6 days gave the best results. The highest regeneration rate was observed on medium containing MS salts with B5 vitamins complemented with 20 g L −1 glucose, 3 mg L −1 BAP-riboside, 0.2 mg L −1 TDZ and 0.2 mg L −1 IBA. This system made it possible to achieve regeneration in each of the varieties examined, though to different extents.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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