Acta Agronomica Hungarica
Volume/Issue:
Volume 57: Issue 3
Effect of heavy metals on the leaf disc ferricyanide reduction in cucumber
Authors:
G. Rabnecz Szent István University Institute of Botany and Ecophysiology Gödöllő Hungary

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,
G. Záray Eötvös Loránd University Research Institute of Environmental Sciences Budapest Hungary

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,
L. Lévai Debrecen University Institute of Crop Sciences, Division of Agricultural Botany and Crop Physiology Debrecen Hungary

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, and
F. Fodor Eötvös Loránd University Department of Plant Physiology and Molecular Plant Biology Budapest Hungary

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Pages:
307–320
Online Publication Date:
20 Nov 2009
Publication Date:
01 Sep 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.57.2009.3.6
Keywords:
Cucumis sativus ; chlorophyll; leaf disc; Pb toxicity; Cd toxicity; iron uptake; apoplasm; symplasm
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The effect of heavy metals on the leaf plasma membrane electron transport systems was investigated in connection with the tissue Fe concentration in Fe-sufficient and Fe-deficient cucumber leaves. Ten M μPb in the nutrient solution inhibited leaf ferricyanide reduction by 20–26%, whereas 10 M μCd had a more drastic effect, with 80–83% inhibition. Ferricyanide reduction decreased by 14% when 1 mM Pb was applied in situ by vacuum infiltration into control leaf discs, whereas it decreased by 40% when 0.1 mM Cd was applied. Ferricyanide reduction was completely inhibited by 1 mM Cd. The ferricyanide reduction values were correlated with the heavy metal, Fe and chlorophyll concentrations in the leaves. A significant linear correlation was only found with the chlorophyll concentration. The data suggest that there are also direct effects on membranebound reductases, but these are of less significance. Using differentially Fe-deficient plants (grown with 0 to 300 nM Fe in the nutrient solution), a chlorophyll concentration of 0.9–1.0 mg g −1 fresh weight was estimated as the threshold for achieving the ferricyanide reduction levels found in the controls.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
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Foundation		 1950
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Founder Magyar Tudományos Akadémia   
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