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  • 1 University of Tabriz Department of Horticultural Sciences, Faculty of Agriculture Tabriz Iran
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Potato, Solanum tuberosum L. (2n = 4x = 48), is an autotetraploid species, the breeding of which at the tetraploid level is complicated by tetrasomic inheritance. Dihaploids (2n = 2x = 24) from the tetraploid cultivated potato have great potential for breeding and genetic studies. The common method deployed to obtain potato dihaploids is to make interspecific-interploidy (4x × 2x) crosses between a tetraploid seed parent and special clones from the diploid S. phureja as pollinator. Pollinators carrying a marker gene have been used, but unfortunately, these clones were very weak, with rare flowering and low male fertility under the given conditions. To find a suitable pollinator, three clones were selected from S. phureja based on flowering, pollen shed and male fertility and were crossed with five cultivated tetraploid potatoes to evaluate their dihaploid induction ability. A total of 1529 interploidy crosses were made, resulting in 1116 berries and 1456 seeds. The progeny were divided into two groups based on stem, flower and tuber colour: hybrids and non-hybrids (putative dihaploids). Chromosome counting in non-hybrid genotypes detected 39 dihaploids. The clone phu 3 and cv. Picasso, with 12.1 and 10.7 dihaploids per 100 berries, respectively, were the best dihaploid inducer and seed parent for dihaploid production.

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