Acta Agronomica Hungarica
Volume/Issue:
Volume 58: Issue 1
Molecular farming, using the cereal endosperm as bioreactor
Author:
L. Tamás Eötvös Loránd University Department of Plant Physiology and Molecular Plant Biology Budapest Hungary

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Pages:
55–64
Online Publication Date:
30 Mar 2010
Publication Date:
01 Mar 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.58.2010.1.7
Keywords:
cereal transformation; edible vaccine; biofermentor; molecular farming
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Seed is an ideal protein production platform because it is the storage organ of the plant and offers appropriate storage compartments for the deposition of foreign proteins. To achieve high foreign protein expression level in the endosperm tissue, the transformation cassette carried the tissue-specific promoter of the wheat high-molecularweight glutenin subunit protein 1Bx17, fused to the first intron of rice actin promoter. Transformation protocols were established and optimized in the laboratory for cereals such as rice, barley and wheat using direct DNA delivery and the Agrobacterium tumefaciens mediated transformation system. Both immature (barley) and mature (rice) embryos, and immature inflorescences (wheat) were used as sources of explants. Subunit edible vaccines were produced to introduce the LTB, CTB and fused LTB-PEDV genes into the rice genome. The PEDV gene was also integrated into the barley genome. A project has recently been started to produce a rabbit-derived enzyme in transgenic wheat endosperm to be used by the pharmaceutical industry.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
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French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
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per Year		  
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per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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