Testing cereal frost tolerance goes back for decades in the Agricultural Research Institute, Martonvásár, Hungary. The climatic programmes used in the plant growth chamber have proved to be fairly efficient, but these methods are time-consuming and have become quite expensive in recent years. An attempt was made to shorten this process by reducing the cold hardening phase, and the freezing test has been simplified and shortened by measuring the relative conductance of leaf segments frozen in a liquid freezer. Frost-tolerant and sensitive wheat lines were tested, and the sensitivity of the system was checked by testing single chromosome substitution lines. Differences were found for all lines frozen at different temperatures. To reduce the costs of the experiment it was attempted to cold-harden the plants not only in a growth chamber but also in a cold room under very low light intensity and it was found that even under thess unfavourable conditions the plants developed a certain level of frost tolerance. The simplified frost tolerance test has proved to be effective, but requires further improvement due to the unsatisfactory significance levels.
Distelfeld, A., Dubcovsky, J. (2010): Characterization of the maintained vegetative phase (mvp) deletions from einkorn wheat and their effect on VRN2 and FT transcript levels. Mol. Genet. Genomics , 283 , 223–232.
Dubcovsky J. , 'Characterization of the maintained vegetative phase (mvp) deletions from einkorn wheat and their effect on VRN2 and FT transcript levels ' (2010 ) 283 Mol. Genet. Genomics : 223 -232 .
Fowler, D. B., Chauvin, L. P., Limin, A. E., Sarhan, F. (1996): The regulatory role of vernalization in the expression of low-temperature-induced genes in wheat and rye. Theor. Appl. Genet. , 93 , 554–559.
Sarhan F. , 'The regulatory role of vernalization in the expression of low-temperature-induced genes in wheat and rye ' (1996 ) 93 Theor. Appl. Genet. : 554 -559 .
Limin. A. E., Fowler, D. B. (1988): Cold hardiness expression in interspecific hybrids and amphiploids of the Triticeae. Genome , 30 , 361–365.
Fowler D. B. , 'Cold hardiness expression in interspecific hybrids and amphiploids of the Triticeae ' (1988 ) 30 Genome : 361 -365 .
Limin, A. E., Fowler, D. B. (2006): Low-temperature tolerance and genetic potential in wheat ( Triticum aestivum L.): response to photoperiod, vernalization, and plant development. Planta , 224 , 360–366.
Fowler D. B. , 'Low-temperature tolerance and genetic potential in wheat (Triticum aestivum L.): response to photoperiod, vernalization, and plant development ' (2006 ) 224 Planta : 360 -366 .
Miuraa, K., Jina, J. B., Leea, J., Yooa, C. Y., Stirma, V., Miuraa, T., Ashworth, E. N., Bressana, R. A., Yunc, D-J., Hasegawaa, P. M. (2007): SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis . The Plant Cell , 19 , 1403–1414.
Hasegawaa P. M. , 'SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis ' (2007 ) 19 The Plant Cell : 1403 -1414 .
Prášil, I., Zámečik, J. (1998): The use of a conductivity measurement method for assessing freezing injury: I. Influence of leakage time, segment number, size and shape in a sample on evaluation of the degree of injury. Env. Exp. Bot. , 40 , 1–10.
Zámečik J. , 'The use of a conductivity measurement method for assessing freezing injury: I. Influence of leakage time, segment number, size and shape in a sample on evaluation of the degree of injury ' (1998 ) 40 Env. Exp. Bot. : 1 -10 .
Reynolds, M. P., Nagarajan, S., Razzaque, M. A., Ageeb, O. A. A. (2001): Cold tolerance. pp. 111–123. In: Reynolds, M. P., Ortiz-Monasterio, J. I., Mc Nab, A. (eds.), Application of Physiology in Wheat Breeding . CIMMYT, Mexico, D.F.
Ageeb O. A. A. , '', in Application of Physiology in Wheat Breeding , (2001 ) -.
Sutka, J. (1981): Genetic studies of frost resistance in wheat. Theor. Appl. Genet. , 59 , 145–152.
Sutka J. , 'Genetic studies of frost resistance in wheat ' (1981 ) 59 Theor. Appl. Genet. : 145 -152 .
Szalai, G., Pap, M., Janda, T. (2009): Light-induced frost tolerance differs in winter and spring wheat plants. J. Plant Physiol. , 166 , 1826–1831.
Janda T. , 'Light-induced frost tolerance differs in winter and spring wheat plants ' (2009 ) 166 J. Plant Physiol. : 1826 -1831 .
Tischner, T., Kőszegi, B., Veisz, O. (1997): Climatic programmes used in the Martonvásár Phytotron most frequently in recent years. Acta Agron. Hung. , 45 , 85–104.
Veisz O. , 'Climatic programmes used in the Martonvásár Phytotron most frequently in recent years ' (1997 ) 45 Acta Agron. Hung. : 85 -104 .
Thomashow, M. F. (1990): Molecular genetics of cold acclimation in higher plants. Adv. Genet. , 28 , 99–131.
Thomashow M. F. , 'Molecular genetics of cold acclimation in higher plants ' (1990 ) 28 Adv. Genet. : 99 -131 .
Thomashow, M. F. (1999): Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Phys. , 50 , 571–599.
Thomashow M. F. , 'Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms ' (1999 ) 50 Annu. Rev. Plant Phys. : 571 -599 .
Trevaskis, B., Tadege, M., Hemming, M. N., Peacock, W. J., Dennis, E. S., Sheldon, C. (2007): Short Vegetative Phase-like MADS-box genes inhibit floral meristem identity in barley. Plant Physiol. , 143 , 225–235.
Sheldon C. , 'Short Vegetative Phase-like MADS-box genes inhibit floral meristem identity in barley ' (2007 ) 143 Plant Physiol. : 225 -235 .
Vágújfalvi, A., Kerepesi, I., Galiba, G., Tischner, T., Sutka, J. (1999): Frost hardiness depending on carbohydrate changes during cold acclimation in wheat. Plant Sci. , 144 , 85–92.
Sutka J. , 'Frost hardiness depending on carbohydrate changes during cold acclimation in wheat ' (1999 ) 144 Plant Sci. : 85 -92 .