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  • 1 Agricultural Research Institue of the Hungarian Academy of Sciences Department of Molecular Plant Biology Martonvásár Hungary
  • | 2 Corvinus University of Budapest Department of Plant Biology and Plant Biochemistry Budapest Hungary
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Testing cereal frost tolerance goes back for decades in the Agricultural Research Institute, Martonvásár, Hungary. The climatic programmes used in the plant growth chamber have proved to be fairly efficient, but these methods are time-consuming and have become quite expensive in recent years. An attempt was made to shorten this process by reducing the cold hardening phase, and the freezing test has been simplified and shortened by measuring the relative conductance of leaf segments frozen in a liquid freezer. Frost-tolerant and sensitive wheat lines were tested, and the sensitivity of the system was checked by testing single chromosome substitution lines. Differences were found for all lines frozen at different temperatures. To reduce the costs of the experiment it was attempted to cold-harden the plants not only in a growth chamber but also in a cold room under very low light intensity and it was found that even under thess unfavourable conditions the plants developed a certain level of frost tolerance. The simplified frost tolerance test has proved to be effective, but requires further improvement due to the unsatisfactory significance levels.

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