Acta Agronomica Hungarica
Volume/Issue:
Volume 58: Issue 2
Investigations on the regeneration ability of squash cultivars
Authors:
E. Kiss-Bába

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,
S. Pánczél

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,
K. Simonyi Corvinus University of Budapest Department of Genetics and Plant Breeding, Faculty of Horticultural Science Budapest Hungary

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, and
G. Bisztray

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Pages:
159–166
Online Publication Date:
17 Jun 2010
Publication Date:
01 Jun 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.58.2010.2.7
Keywords:
in vitro ; regeneration; organogenesis; cotyledon; squash; zucchini; pumpkin
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Pumpkin, squash and zucchini are important vegetable crops in tropical and temperate regions. The development of genetic transformation methods offers the potential of introducing valuable traits into these crops. An efficient in vitro plant regeneration system is a critical point for genetic manipulation. The regeneration ability of three Cucurbita varieties was tested on Murashige and Skoog medium supplemented with different growth regulators. Cotyledons of all the varieties were cultured to investigate the effect of 2,4-D (0, 1, 2, 3, 4 mg l −1 ) with or without KIN (0, 0.5, 5 mg l −1 ) and of BA (0, 1, 1.2 mg l −1 ) combined with IAA (0, 0.9, 1, 1.2 mg l −1 ), on the efficiency of shoot induction. Abscisic acid (0.26 mg l −1 ABA) was also added to one medium. To find the most suitable combination for shoot induction, cotyledon segments of the three varieties were also cultivated on media with different concentrations of BA (0–1.2 mg l −1 ) and IAA (0–0.9 mg l −1 ). Shoot induction was achieved via organogenesis in the tested varieties. Leafy shoots were transferred to root induction media. Regenerated plantlets with roots were transferred to sterile soil. This is the first report on in vitro regeneration from cotyledon explants of the pumpkin cultivar Nagydobosi and the pattypan squash cultivar Óvári fehér.

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Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
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French
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Year of
Foundation		 1950
Publication
Programme		 ceased
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per Year		  
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per Year		  
Founder Magyar Tudományos Akadémia   
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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