Acta Agronomica Hungarica
Volume/Issue:
Volume 58: Issue 3
Visualization of U and M genome chromosomes by multicolour genomic in situ hybridization in Aegilops biuncialis and Triticum aestivum-Ae. biuncialis amphiploids
Authors:
I. Molnár Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár Hungary

Search for other papers by I. Molnár in
Current site
Google Scholar
PubMed Close
and
M. Molnár-Láng Agricultural Research Institute of the Hungarian Academy of Sciences Martonvásár Hungary

Search for other papers by M. Molnár-Láng in
Current site
Google Scholar
PubMed Close
Pages:
195–202
Online Publication Date:
17 Sep 2010
Publication Date:
01 Sep 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/AAgr.58.2010.3.1
Restricted access

The multicolour genomic in situ hybridization (mcGISH) method was improved in order to visualize the U b and M b genomes of Aegilops biuncialis Vis. (2n=4x=28, U b U b M b M b ). Hybridization probes prepared from the diploid U and M genome donors, Ae. umbellulata and Ae. comosa , resulted in clear hybridization signals on the U and M chromosomes in Ae. biuncialis . The random primed labelling method made it possible to decrease the blocking ratio to 1:30. McGISH allowed the simultaneous discrimination of individual Ae. biuncialis genomes and wheat chromosomes in γ-irradiated Triticum aestivum-Ae. biuncialis amphiploids (2n=70; AABBDDU b U b M b M b ). Dicentric chromosomes, terminal and interstitial translocations and centric fusions were detected in the irradiated generation. The irradiation-induced wheat- Ae. biuncialis intergenomic translocations will facilitate the successful introgression of useful agronomic traits into bread wheat.

  • Aghaee-Sarbarzeh, M., Ferrahi, M., Singh, S., Singh, H., Friebe, B., Gill, B. S., Dhaliwal, H. S. (2002): Ph1 induced transfer of leaf and stripe rust-resistance genes from Aegilops triuncialis and Ae. geniculata to bread wheat. Euphytica , 127 , 377–382.

    Dhaliwal H. S. , 'Ph1 induced transfer of leaf and stripe rust-resistance genes from Aegilops triuncialis and Ae. geniculata to bread wheat ' (2002 ) 127 Euphytica : 377 -382 .

    • Search Google Scholar

  • Badaeva, E. D., Friebe, B., Gill, B. S. (1996): Genome differentiation in Aegilops . 1. Distribution of highly repetitive DNA sequences on chromosomes of diploid species. Genome , 39 , 293–306.

    Gill B. S. , 'Genome differentiation in Aegilops. 1. Distribution of highly repetitive DNA sequences on chromosomes of diploid species ' (1996 ) 39 Genome : 293 -306 .

    • Search Google Scholar

  • Badaeva, E. D., Dedkova, O. S., Gay, G., Pukhalskyi, V. A., Zelenin, A. V., Bernard, S., Bernard, M. (2007): Chromosomal rearrangements in wheat: their types and distribution. Genome , 50 , 907–926.

    Bernard M. , 'Chromosomal rearrangements in wheat: their types and distribution ' (2007 ) 50 Genome : 907 -926 .

    • Search Google Scholar

  • Belyayev, A., Raskina, O., Nevo, E. (2001): Detection of alien chromosomes from S-genome species in the addition/substitution lines of bread wheat and visualization of A-, B- and Dgenomes by GISH. Hereditas , 135 , 119–122.

    Nevo E. , 'Detection of alien chromosomes from S-genome species in the addition/substitution lines of bread wheat and visualization of A-, B- and Dgenomes by GISH ' (2001 ) 135 Hereditas : 119 -122 .

    • Search Google Scholar

  • Benavente, E., Alix, K., Dusautoir, J. C., Orellana, J., David, J. L. (2001): Early evolution of the chromosomal structure of Triticum turgidum - Aegilops ovata amphiploids carrying and lacking the Ph1 gene. Theor. Appl. Genet. , 103 , 1123–1128.

    David J. L. , 'Early evolution of the chromosomal structure of Triticum turgidum-Aegilops ovata amphiploids carrying and lacking the Ph1 gene ' (2001 ) 103 Theor. Appl. Genet. : 1123 -1128 .

    • Search Google Scholar

  • Beskid, O., Dusek, Z., Solansky, I., Sram, R. J. (2006): The effects of exposure to different clastogens on the pattern of chromosomal aberrations detected by FISH whole chromosome painting in occupationally exposed individuals. Mutat. Res. Fund. Mol. M. , 594 , 20–29.

    Sram R. J. , 'The effects of exposure to different clastogens on the pattern of chromosomal aberrations detected by FISH whole chromosome painting in occupationally exposed individuals ' (2006 ) 594 Mutat. Res. Fund. Mol. M. : 20 -29 .

    • Search Google Scholar

  • Blackburn, E. H., Szostak, J. W. (1984): The molecular structure of centromeres and telomeres. Annu. Rev. Biochem. , 53 , 163–194.

    Szostak J. W. , 'The molecular structure of centromeres and telomeres ' (1984 ) 53 Annu. Rev. Biochem. : 163 -194 .

    • Search Google Scholar

  • Colmer, T. D., Flowers, T. J., Munns, R. (2006): Use of wild relatives to improve salt tolerance in wheat. J. Exp. Bot. , 57 , 1059–1078.

    Munns R. , 'Use of wild relatives to improve salt tolerance in wheat ' (2006 ) 57 J. Exp. Bot. : 1059 -1078 .

    • Search Google Scholar

  • Damania, A. B., Pecetti, L. (1990): Variability in a collection of Aegilops species and evaluation for yellow rust resistance at two locations in Northern Syria. J. Genet. Breed. , 44 , 97–102.

    Pecetti L. , 'Variability in a collection of Aegilops species and evaluation for yellow rust resistance at two locations in Northern Syria ' (1990 ) 44 J. Genet. Breed. : 97 -102 .

    • Search Google Scholar

  • Dulai, S., Molnár, I., Prónay, J., Marschall, M., Csernák, Á., Tarnai, R., Molnár-Láng, M. (2005): Effects of drought on thermal stability of photosynthetic apparatus in bread wheat and in Aegilops species originating from various habitats. Acta Biol. Szeg. , 49 , 215–217.

    Molnár-Láng M. , 'Effects of drought on thermal stability of photosynthetic apparatus in bread wheat and in Aegilops species originating from various habitats ' (2005 ) 49 Acta Biol. Szeg. : 215 -217 .

    • Search Google Scholar

  • Ekmekci, Y., Terzioglu, S. (2002): Changes in the electrophoretic pattern of soluble shoot proteins of wild and cultivated tetraploid wheats following cold acclimation and freezing. Israel J. Plant Sci. , 50 , 95–102.

    Terzioglu S. , 'Changes in the electrophoretic pattern of soluble shoot proteins of wild and cultivated tetraploid wheats following cold acclimation and freezing ' (2002 ) 50 Israel J. Plant Sci. : 95 -102 .

    • Search Google Scholar

  • Forsberg, J., Dixelius, C., Lagercrantz, U., Glimelius, K. (1998): UV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana . Plant Sci. , 131 , 65–76.

    Glimelius K. , 'UV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana ' (1998 ) 131 Plant Sci. : 65 -76 .

    • Search Google Scholar

  • Friebe, B., Hatchett, J. H., Gill, B. S., Sebesta, E. E. (1991): Transfer of hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations. Theor. Appl. Genet. , 83 , 33–40.

    Sebesta E. E. , 'Transfer of hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations ' (1991 ) 83 Theor. Appl. Genet. : 33 -40 .

    • Search Google Scholar

  • Friebe, B., Jiang, J., Raupp, W. J., McIntosh, R. A., Gill, B. S. (1996): Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica , 91 , 59–87.

    Gill B. S. , 'Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status ' (1996 ) 91 Euphytica : 59 -87 .

    • Search Google Scholar

  • Gill, B. S. (1983): Tomato cytogenetics: A search for new frontiers. pp. 457-480. In: Swaminathan, M. S., Gupta, P. K., Sinha, U. (eds.), Cytogenetics of Crop Plants . Macmillan India, New Delhi.

    Gill B. S. , '', in Cytogenetics of Crop Plants , (1983 ) -.

  • Han, F. P., Fedak, G., Benabdelmouna, A., Armstrong, K., Ouellet, T. (2003): Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH. Genome , 46 , 490–495.

    Ouellet T. , 'Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH ' (2003 ) 46 Genome : 490 -495 .

    • Search Google Scholar

  • Jiang, J. M., Friebe, B., Gill, B. S. (1994): Recent advances in alien gene-transfer in wheat. Euphytica , 73 , 199–212.

    Gill B. S. , 'Recent advances in alien gene-transfer in wheat ' (1994 ) 73 Euphytica : 199 -212 .

    • Search Google Scholar

  • Knott, D. R. (1987): Transferring alien genes to wheat. pp. 462–471. In: Heyne, E. G. (ed.), Wheat and Wheat Improvement . 2nd edn. American Society of Agronomy, Monograph 13.

    Knott D. R. , '', in Wheat and Wheat Improvement , (1987 ) -.

  • Kunzel, G., Gecheff, K. I., Schubert, I. (2001): Different chromosomal distribution patterns of radiation-induced interchange breakpoints in barley: first post-treatment mitosis versus viable offspring. Genome , 44 , 128–132.

    Schubert I. , 'Different chromosomal distribution patterns of radiation-induced interchange breakpoints in barley: first post-treatment mitosis versus viable offspring ' (2001 ) 44 Genome : 128 -132 .

    • Search Google Scholar

  • Leblon, G., Zickler, D., Lebilcot, S. (1986): Most UV-induced reciprocal translocations in Sordaria macrospora occur in or near centromere regions. Genetics , 112 , 183–204.

    Lebilcot S. , 'Most UV-induced reciprocal translocations in Sordaria macrospora occur in or near centromere regions ' (1986 ) 112 Genetics : 183 -204 .

    • Search Google Scholar

  • Makkouk, K. M., Comeau, A., Ghulam, W. (1994): Resistance to barley yellow dwarf luteovirus in Aegilops species. Can. J. Plant Sci. , 74 , 631–634.

    Ghulam W. , 'Resistance to barley yellow dwarf luteovirus in Aegilops species ' (1994 ) 74 Can. J. Plant Sci. : 631 -634 .

    • Search Google Scholar

  • Molnár, I., Dulai, S., Molnár-Láng, M. (2008): Can the drought tolerance traits of Ae. Biuncialis manifest even in the wheat genetic background? Acta Biol. Szeg. , 52 , 175–178.

    Molnár-Láng M. , 'Can the drought tolerance traits of Ae. Biuncialis manifest even in the wheat genetic background? ' (2008 ) 52 Acta Biol. Szeg. : 175 -178 .

    • Search Google Scholar

  • Molnár, I., Gáspár, L., Sárvári, É., Dulai, S., Hoffmann, B., Molnár-Láng, M., Galiba, G. (2004): Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought. Funct. Plant Biol. , 31 , 1149–1159.

    Galiba G. , 'Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought ' (2004 ) 31 Funct. Plant Biol. : 1149 -1159 .

    • Search Google Scholar

  • Molnár, I., Schneider, A., Molnár-Láng, M. (2005): Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences. Cereal Res. Commun. , 33 , 673–680.

    Molnár-Láng M. , 'Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences ' (2005 ) 33 Cereal Res. Commun. : 673 -680 .

    • Search Google Scholar

  • Molnár-Láng, M., Linc, G., Sutka, J. (1996): Transfer of the recessive crossability allele kr1 from Chinese Spring into the winter wheat variety Martonvásári 9. Euphytica , 90 , 301–305.

    Sutka J. , 'Transfer of the recessive crossability allele kr1 from Chinese Spring into the winter wheat variety Martonvásári 9 ' (1996 ) 90 Euphytica : 301 -305 .

    • Search Google Scholar

  • Mukai, Y., Nakahara, Y., Yamamoto, M. (1993): Simultaneous discrimination of the 3 genomes in hexaploid wheat by multicolour fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome , 36 , 489–494.

    Yamamoto M. , 'Simultaneous discrimination of the 3 genomes in hexaploid wheat by multicolour fluorescence in situ hybridization using total genomic and highly repeated DNA probes ' (1993 ) 36 Genome : 489 -494 .

    • Search Google Scholar

  • Natarajan, A. T. (2002): Chromosome aberrations: past, present and future. Mutat. Res. Fund. Mol. M. , 504 , 3–16.

    Natarajan A. T. , 'Chromosome aberrations: past, present and future ' (2002 ) 504 Mutat. Res. Fund. Mol. M. : 3 -16 .

    • Search Google Scholar

  • Rao, M. K., Koduru, P. R. K. (1977): Asynapsis and spontaneous centromeric breakage in an inbred line of Pennisetum americanum (L.) Leeke. P. Indian. AS. B. , 87 , 29–35.

    Koduru P. R. K. , 'Asynapsis and spontaneous centromeric breakage in an inbred line of Pennisetum americanum (L.) Leeke ' (1977 ) 87 P. Indian. AS. B. : 29 -35 .

    • Search Google Scholar

  • Riera-Lizarazu, O., Vales, M. I., Ananiev, E. V., Rines, H. W., Phillips, R. L. (2000): Production and characterization of maize chromosome 9 radiation hybrids derived from an oat-maize addition line. Genetics , 156 , 327–339.

    Phillips R. L. , 'Production and characterization of maize chromosome 9 radiation hybrids derived from an oat-maize addition line ' (2000 ) 156 Genetics : 327 -339 .

    • Search Google Scholar

  • Sánchez-Morán, E., Benavente, E., Orellana, J. (1999): Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives. Heredity , 83 , 249–252.

    Orellana J. , 'Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives ' (1999 ) 83 Heredity : 249 -252 .

    • Search Google Scholar

  • Schneider, A., Linc, G., Molnár, I., Molnár-Láng, M. (2005): Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of five derived wheat/ Aegilops biuncialis disomic addition lines. Genome , 48 , 1070–1082.

    Molnár-Láng M. , 'Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of five derived wheat/Aegilops biuncialis disomic addition lines ' (2005 ) 48 Genome : 1070 -1082 .

    • Search Google Scholar

  • Sears, E. R. (1956): The transfer of leaf-rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp. Biol. , 9 , 1–22.

    Sears E. R. , 'The transfer of leaf-rust resistance from Aegilops umbellulata to wheat ' (1956 ) 9 Brookhaven Symp. Biol. : 1 -22 .

    • Search Google Scholar

  • Sepsi, A., Molnár, I., Szalay, D., Molnár-Láng, M. (2008): Characterization of a leaf rust resistant wheat- Thinopyrum ponticum partial amphiploid BE-1 using sequential multicolor GISH and FISH. Theor. Appl. Genet. , 116 , 825–834.

    Molnár-Láng M. , 'Characterization of a leaf rust resistant wheat-Thinopyrum ponticum partial amphiploid BE-1 using sequential multicolor GISH and FISH ' (2008 ) 116 Theor. Appl. Genet. : 825 -834 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527

Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation		 1950
Publication
Programme		 ceased
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia   
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2023 0 0 0
Jul 2023 2 0 0
Aug 2023 6 0 0
Sep 2023 4 0 0
Oct 2023 0 2 0
Nov 2023 2 4 0
Dec 2023 4 0 0