Authors:
D. Polgári Szent István University Institute of Genetics and Biotechnology Gödöllő Hungary

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B. Kalapos Szent István University Institute of Genetics and Biotechnology Gödöllő Hungary

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V. Tisza

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L. Kovács Szent István University Institute of Genetics and Biotechnology Gödöllő Hungary

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B. Kerti Szent István University Institute of Genetics and Biotechnology Gödöllő Hungary

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L. Heszky Szent István University Institute of Genetics and Biotechnology Gödöllő Hungary

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E. Kiss Szent István University Institute of Genetics and Biotechnology Gödöllő Hungary

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The aim of this study was to characterize a gene associated with ripening in strawberry, a non-climacteric fruit. Differently expressed transcripts of candidate genes functioning in fruit development and ripening were identified from strawberry ( Fragaria × ananassa Duch.) in four ripening stages using the cDNA-AFLP method. The cDNA fragment designated C11M32M003 was selected from the putative ripening-related genes for further analysis. This transcript accumulated in the green receptacle, and the achene, but gene expression decreased in both tissues in parallel with the progress of ripening (Balogh, 2006). In silico analysis revealed that both the cDNA-AFLP fragment (C11M32M003) and the full-length cDNA AY695666 showed over 60% homology at the nucleotide level with two gene groups found in various plant species, including Arabidopsis thaliana . One of the candidate groups consisted of NITRILASE sequences thought to be related to auxin biosynthesis. As an alternative, a lesser known gene group named SPIRAL was suggested. The results of the detailed bioinformatic comparisons presented in this paper prove that the strawberry sequence analysed belongs to the SPIRAL gene family.

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  • Balogh, A., Koncz, T., Tisza, V., Kiss, E., Heszky, L. (2005b): Identification of genes and their promoters involved in strawberry fruit development and ripening. Kertgazdaság , Special Edition, 105–110.

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  • Yao, M., Wakamatsu, Y., Itoh, T. J., Shoji, T., Hashimoto, T. (2008): Arabidopsis SPIRAL 2 promotes uninterrupted microtubule growth by suppressing the pause state of microtubule dynamics. J. Cell Sci. , 121 , 2372–2381.

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Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation
1950
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia   
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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