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  • 1 CSK Himachal Pradesh Agricultural University Molecular Cytogenetics and Tissue Culture Lab., Department of Plant Breeding and Genetics Palampur India
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The relative efficiency of in vitro and in vivo screening techniques for drought tolerance, comprising various parameters, namely germination (%), shoot length, root length, coleoptile length, root number, root/shoot ratio and seedling vigour index (SVI) under in vitro conditions and morpho-physiological and yield-contributing traits under in vivo conditions, was studied using 78 winter × spring wheat-derived doubled haploid lines of bread wheat along with 13 parental genotypes and two check varieties, HPW 155 and PBW 343. Analysis of variance for different in vitro parameters in control (0 MPa) and stress (−0.7 MPa) environments and various in vivo parameters under irrigated and rainfed environments indicated sufficient genetic variability and the differential response of the genotypes to the different stress levels for all the in vitro and in vivo parameters. Correlation studies revealed the significance of percentage germination, root number, coleoptile length and seedling vigour index under in vitro conditions and relative water content and excised leaf water loss under in vivo conditions as important selection criteria for drought tolerance, as these parameters were related with each other as well as with the drought susceptibility index (S). The significant positive rank correlation between the in vitro (−0.7 MPa) and in vivo (rainfed) stress conditions indicated that the performance of a genotype under field conditions is very similar to its performance under laboratory conditions. Hence, the selection precision for a crucial and complex trait like drought tolerance in wheat can be enhanced by exercising in vitro selection coupled with evaluation in the field. The drought susceptibility index ‘S’ should not be taken as the sole criterion to categorize genotypes as drought-tolerant or susceptible ones.

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Acta Agronomica Hungarica
Language English
Russian
German
French
Size  
Year of
Foundation
1950
Publication
Programme
ceased
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-0161 (Print)
ISSN 1588-2527 (Online)

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