View More View Less
  • 1 Bulgarian Academy of Sciences Institute of Plant Physiology and Genetics Sofia Bulgaria
Restricted access

Wheat cultivars were grown as soil culture under normal growth conditions. Twoweek- old seedlings were exposed to 4°C for 6 h and then transferred to −12°C for 24 h in the dark. Twenty-four hours before freezing stress, some of the plants were sprayed with aqueous solutions of spermine, spermidine, putrescine, 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA). The data showed that freezing stress caused a decrease in the fresh weight, chlorophyll content and plant survival rate, accompanied by a simultaneous accumulation of free proline and the enhanced leakage of electrolytes. Preliminary treatment with polyamines caused a decline in electrolyte leakage and a considerable augmentation in proline quantity, indicating that the compounds are capable of preventing frost injury. Additionally, the foliar application of polyamines retarded the destruction of chlorophyll, and lessened fresh weight losses due to freezing stress. The synthetic triamine DETA was the most effective, having the most pronounced action in all the experiments, followed by the tetraamine spermine. The application of polyamines to wheat crops could be a promising approach for improving plant growth under unfavourable growth conditions, including freezing temperatures. The results demonstrate that treatment with polyamines could protect winter wheat by reducing the stress injuries caused by subzero temperatures.

  • Alcazar, R., Cuevas, J. C., Planas, J., Zarza, X., Bortolotti, C., Carrasco, P., Salinas, J., Tiburcio, A. F., Altabella, T. (2011): Integration of polyamines in the cold acclimation response. Plant Sci., 180, 31–38.

    Altabella T. , 'Integration of polyamines in the cold acclimation response ' (2011 ) 180 Plant Sci. : 31 -38.

    • Search Google Scholar
  • Alexieva, V. (1994a): Chemical structure - plant growth regulating activity of some naturally occurring and synthetic aliphatic amines. Compt. Rend. Acad. Bulg. Sci., 47, 79–82.

    Alexieva V. , 'Chemical structure - plant growth regulating activity of some naturally occurring and synthetic aliphatic amines ' (1994 ) 47 Compt. Rend. Acad. Bulg. Sci. : 79 -82.

    • Search Google Scholar
  • Alexieva, V. (1994b): Effect of exogenous putrescine and its synthetic structural analogues on leaf senescence. Compt. Rend. Acad. Bulg. Sci., 47, 57–60.

    Alexieva V. , 'Effect of exogenous putrescine and its synthetic structural analogues on leaf senescence ' (1994 ) 47 Compt. Rend. Acad. Bulg. Sci. : 57 -60.

    • Search Google Scholar
  • Arnon, D. I. (1949): Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol., 24, 1–15.

    Arnon D. I. , 'Copper enzymes in isolated chloroplasts ' (1949 ) 24 Polyphenol oxidase in Beta vulgaris. Plant Physiol. : 1 -15.

    • Search Google Scholar
  • Bates, L. S., Waldren, R. P., Teare, J. D. (1973): Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205–207.

    Teare J. D. , 'Rapid determination of free proline for water stress studies ' (1973 ) 39 Plant and Soil : 205 -207.

    • Search Google Scholar
  • Bouchereau, A., Aziz, A., Larher, F., Martin-Tanguy, J. (1999): Polyamines and environmental challenges: recent development. Plant Sci., 140, 103–125.

    Martin-Tanguy J. , 'Polyamines and environmental challenges: recent development ' (1999 ) 140 Plant Sci. : 103 -125.

    • Search Google Scholar
  • Dörffling, K., Schulenburg, S., Lesselich, G., Dörffling, H. (1990): Abscisic acid and proline levels in cold hardened winter wheat leaves in relation to variety-specific differences in freezing resistance. J. Agron. Crop Sci., 165, 230–239.

    Dörffling H. , 'Abscisic acid and proline levels in cold hardened winter wheat leaves in relation to variety-specific differences in freezing resistance ' (1990 ) 165 J. Agron. Crop Sci. : 230 -239.

    • Search Google Scholar
  • Fuller, B. J. (2004): Cryoprotectants: the essential antifreezes to protect life in the frozen state. CryoLett., 25, 375–388.

    Fuller B. J. , 'Cryoprotectants: the essential antifreezes to protect life in the frozen state ' (2004 ) 25 CryoLett. : 375 -388.

    • Search Google Scholar
  • Gill, S. S., Tuteja, N. (2010): Polyamines and abiotic stress tolerance in plants. Plant Signaling & Behavior, 5, 26–33.

    Tuteja N. , 'Polyamines and abiotic stress tolerance in plants ' (2010 ) 5 Plant Signaling & Behavior : 26 -33.

    • Search Google Scholar
  • Groppa, M. D., Benavides, M. P. (2008): Polyamines and abiotic stress: recent advances. Amino Acids, 34, 35–45.

    Benavides M. P. , 'Polyamines and abiotic stress: recent advances ' (2008 ) 34 Amino Acids : 35 -45.

    • Search Google Scholar
  • Gusta, L. V., Trischuk, R., Weiser, C. J. (2005): Plant cold acclimation: the role of abscisic acid. J. Plant Growth Regul., 24, 308–318.

    Weiser C. J. , 'Plant cold acclimation: the role of abscisic acid ' (2005 ) 24 J. Plant Growth Regul. : 308 -318.

    • Search Google Scholar
  • Ha, H. C., Sirisoma, N. S., Kuppusamy, P., Zweiler, J. L., Woster, P. M., Casero, R. A. Jr. (1998): The natural polyamine spermine functions directly as a free scavenger. PNAS, 95, 11140–11145.

    Casero R. A. , 'The natural polyamine spermine functions directly as a free scavenger ' (1998 ) 95 PNAS : 11140 -11145.

    • Search Google Scholar
  • Jeon, J., Kim, N. Y., Kim, S., Kang, N. Y., Novak, O., Ku, S.-J., Cho, C., Lee, D. J., Lee, E.-J., Strnad, M., Kim, J. (2010): A subset of cytokinin two-component signaling system plays a role in cold temperature stress response in Arabidopsis. J. Biol. Chem., 285, 23371–23386.

    Kim J. , 'A subset of cytokinin two-component signaling system plays a role in cold temperature stress response in Arabidopsis ' (2010 ) 285 J. Biol. Chem. : 23371 -23386.

    • Search Google Scholar
  • Kocsy, G., Pál, M., Soltész, A., Szalai, G., Boldizsár, Á., Kovács, V., Janda, T. (2011a): Low temperature and oxidative stress in cereals. Acta Agron. Hung., 59, 169–189.

    Janda T. , 'Low temperature and oxidative stress in cereals ' (2011 ) 59 Acta Agron. Hung. : 169 -189.

    • Search Google Scholar
  • Kocsy, G., Simon-Sarkadi, L., Kovács, Z., Boldizsár, Á., Sovány, C., Kirsch, K., Galiba, G. (2011b): Regulation of free amino acid and polyamine levels during cold acclimation in wheat. Acta Biologica Szegediensis, 55, 91–93.

    Galiba G. , 'Regulation of free amino acid and polyamine levels during cold acclimation in wheat ' (2011 ) 55 Acta Biologica Szegediensis : 91 -93.

    • Search Google Scholar
  • Kovács, Z., Simon-Sarkadi, L., Sovány, C., Kirsch, K., Galiba, G., Kocsy, G. (2011): Differential effects of cold acclimation and abscisic acid on free amino acid composition in wheat. Plant Science, 180, 61–68.

    Kocsy G. , 'Differential effects of cold acclimation and abscisic acid on free amino acid composition in wheat ' (2011 ) 180 Plant Science : 61 -68.

    • Search Google Scholar
  • Langridge, P., Paltridge, N., Fincher, G. (2006): Functional genomics of abiotic stress tolerance in cereals. Briefings in Functional Genomics and Proteomics, 4, 343–354.

    Fincher G. , 'Functional genomics of abiotic stress tolerance in cereals ' (2006 ) 4 Briefings in Functional Genomics and Proteomics : 343 -354.

    • Search Google Scholar
  • Macháčková, I., Hanišová, A., Krekule, J. (2006): Levels of ethylene, ACC, MACC, ABA and proline as indicators of cold hardening and frost resistance in winter wheat. Physiol. Plant., 76, 603–607.

    Krekule J. , 'Levels of ethylene, ACC, MACC, ABA and proline as indicators of cold hardening and frost resistance in winter wheat ' (2006 ) 76 Physiol. Plant. : 603 -607.

    • Search Google Scholar
  • Mansour, M. M. F. (2000): Nitrogen containing compounds and adaptation of plants to salinity stress. Biol. Plant., 43, 491–500.

    Mansour M. M. F. , 'Nitrogen containing compounds and adaptation of plants to salinity stress ' (2000 ) 43 Biol. Plant. : 491 -500.

    • Search Google Scholar
  • Parvanova, D., Ivanov, S., Konstantinova, T., Karanov, E., Atanassov, A., Tsvetkov, T., Alexieva, V., Djilianov, D. (2004): Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress, Plant Physiol. Biochem., 42, 57–63.

    Djilianov D. , 'Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress, Plant Physiol ' (2004 ) 42 Biochem. : 57 -63.

    • Search Google Scholar
  • Rai, V. K. (2002): Role of amino acids in plant responses to stresses. Biol. Plant., 45, 481–487.

    Rai V. K. , 'Role of amino acids in plant responses to stresses ' (2002 ) 45 Biol. Plant. : 481 -487.

    • Search Google Scholar
  • Ramin, A. A. (2009): Improving germination performance and chilling tolerance in cucumber seedlings with paclobutrazol. Int. J. Veg. Sci., 15, 173–184.

    Ramin A. A. , 'Improving germination performance and chilling tolerance in cucumber seedlings with paclobutrazol ' (2009 ) 15 Int. J. Veg. Sci. : 173 -184.

    • Search Google Scholar
  • Sãulescu, N. N., Braun, H.-J. (2001): Cold tolerance. pp. 111–123. In: Reynolds, M. P., Ortiz-Monasterio, J. I., McNab, A. (eds.), Application of Physiology in Wheat Breeding. CIMMYT, Mexico D.F., Mexico.

    Braun H.-J. , '', in Application of Physiology in Wheat Breeding , (2001 ) -.

  • Senaratna, T., Mackay, C., McKersie, B., Fletcher, R. (1988): Uniconasole-induced chilling tolerance to tomato and its relationship to antioxidant content. J. Plant Physiol., 133, 56–61.

    Fletcher R. , 'Uniconasole-induced chilling tolerance to tomato and its relationship to antioxidant content ' (1988 ) 133 J. Plant Physiol. : 56 -61.

    • Search Google Scholar
  • Thomashow, M. F. (1990): Molecular genetics of cold acclimation of higher plants. Adv. Gen., 28, 99–131.

    Thomashow M. F. , 'Molecular genetics of cold acclimation of higher plants ' (1990 ) 28 Adv. Gen. : 99 -131.

    • Search Google Scholar
  • Thomashow, M. F. (1999): Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Ann Rev. Plant. Physiol. Plant Mol. Biol., 50, 571–599.

    Thomashow M. F. , 'Plant cold acclimation: freezing tolerance genes and regulatory mechanisms ' (1999 ) 50 Ann Rev. Plant. Physiol. Plant Mol. Biol. : 571 -599.

    • Search Google Scholar
  • Todorova, D., Moskova, I., Sergiev, I., Alexieva, V., Mapelli, S. (2008): Changes in endogenous polyamines and some stress markers content induced by drought, 4PU-30 and abscisic acid in wheat plants. pp. 205–215. In: Khan, N., Singh, S. (eds.), Abiotic Stress and Plant Responses. I. K. International Publishing House, New Delhi.

    Mapelli S. , '', in Abiotic Stress and Plant Responses , (2008 ) -.

  • Tsonev, T., Sergiev, I. (1993): Leaf area measurements using hand scanner. Photosyntetica, 29, 625–630.

    Sergiev I. , 'Leaf area measurements using hand scanner ' (1993 ) 29 Photosyntetica : 625 -630.

    • Search Google Scholar
  • Wang, L. J., Chen, S. J., Kong, W. F., Li, S. H., Archbold, D. D. (2006): Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during storage. Postharv. Biol. Technol., 41, 244–251.

    Archbold D. D. , 'Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during storage ' (2006 ) 41 Postharv. Biol. Technol. : 244 -251.

    • Search Google Scholar
  • Wang, X., Shi, G., Xu, Q., Hu, J. (2007): Exogenous polyamines enhance copper tolerance of Nymphoides peltatum. J. Plant Physiol., 164, 1062–1070.

    Hu J. , 'Exogenous polyamines enhance copper tolerance of Nymphoides peltatum ' (2007 ) 164 J. Plant Physiol. : 1062 -1070.

    • Search Google Scholar
  • Wise, R. (1995): Chilling-enhanced photooxidation: The production, action and study of reactive oxygen species produced during chilling in the light. Photosynth. Res., 45, 79–97.

    Wise R. , 'Chilling-enhanced photooxidation: The production, action and study of reactive oxygen species produced during chilling in the light ' (1995 ) 45 Photosynth. Res. : 79 -97.

    • Search Google Scholar
  • Yadav, S. K. (2010): Cold stress tolerance mechanisms in plants. A review. Agron. Sustain. Dev., 30, 515–527.

    Yadav S. K. , 'Cold stress tolerance mechanisms in plants ' (2010 ) 30 A review. Agron. Sustain. Dev. : 515 -527.

    • Search Google Scholar
  • Yelenosky, G. (1979): Accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes. Plant Physiol., 64, 425–427.

    Yelenosky G. , 'Accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes ' (1979 ) 64 Plant Physiol. : 425 -427.

    • Search Google Scholar
  • Zhang, W., Jiang, B., Li, W., Song, H., Yu, Y., Chen, J. (2009): Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system. Sci. Horticult., 122, 200–208.

    Chen J. , 'Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system ' (2009 ) 122 Sci. Horticult. : 200 -208.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 3 0 0
Sep 2020 0 0 0
Oct 2020 0 0 0
Nov 2020 1 1 0
Dec 2020 3 0 0
Jan 2021 2 0 0
Feb 2021 0 0 0