The response given by the photosynthetic pigments, antioxidant enzyme activity and nutrient accumulation of kinnow (Citrus nobilis × C. deliciosa) budded on Citrus jambhiri Lush rootstocks (var. Jatti Khatti) to triazole compounds was studied under NaCl stress. Eighteen-month-old budded kinnow plants were irrigated with 0 or 50 mM NaCl with or without triazole compounds. Paclobutrazol (PBZ) at 100 mg 1−1 and triazole (TRZ), triadimefon (TDM) and hexaconazole (HEX) at 20 mg l−1 were applied by drenching the soil one week prior to NaCl application. PBZ, TRZ and TDM were equally effective in preventing chlorophyll degradation under NaCl stress. The improvement in catalase (CAT) activity was greater in TRZ- and TDM-treated plants under stress, while for superoxide dismutase (SOD) activity, TRZ, PBZ and TDM were found to be equally effective. The peroxidase (POX) activity increased more in TDM-treated plants. The application of TDM and HEX had a more pronounced effect on the upregulation of proline content under stress. Furthermore, triazole compounds increased the accumulation of K+ and Ca2+ ions and inhibited that of Na+ and Cl−, resulting in an improvement in the K+/Na+ and K+/Cl− ratios. For PBZ-treated plants, the K+/Na+ and K+/Cl− ratios in leaf tissues increased 2.4- and 2.1-fold compared to plants treated with NaCl alone, while HEX had a more striking effect on Ca2+ upregulation and significantly (1.5-fold) increased leaf Ca2+ under stress.
Adriano, D. C., Doner, H. E. (1982): Bromine, chlorine and fluorine. In: Page, A. L., Miller, R. H., Keency, D. R. (eds.), Methods of Soil Analysis Part II. Chemical and Biological Properties. Soil Science Society of America and American Society of Agronomy, Madison, Wisconsin, USA, pp. 461–462.
Doner H. E. , '', in Methods of Soil Analysis Part II. Chemical and Biological Properties , (1982 ) -.
Asare-Boamah, N. K., Hofstra, G., Fletcher, R. A., Dumbroff, E. B. (1986): Triadimefon protects bean plants from water stress through its effects on abscisic acid. Plant Cell Physiol., 27, 383–390.
Dumbroff E. B. , 'Triadimefon protects bean plants from water stress through its effects on abscisic acid ' (1986 ) 27 Plant Cell Physiol. : 383 -390.
Asare-Boamah, N. K., Fletcher, R. A. (1986): Protection of bean seedlings against heat and chilling injury by triadimefon. Physiol Plant., 67, 353–358.
Fletcher R. A. , 'Protection of bean seedlings against heat and chilling injury by triadimefon ' (1986 ) 67 Physiol Plant. : 353 -358.
Bates, L. S., Waldren, R. D., Teare, I. D. (1973): Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205–207.
Teare I. D. , 'Rapid determination of free proline for water stress studies ' (1973 ) 39 Plant and Soil : 205 -207.
Blum, A., Ebercon, A. (1981): Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci., 21, 43–47.
Ebercon A. , 'Cell membrane stability as a measure of drought and heat tolerance in wheat ' (1981 ) 21 Crop Sci. : 43 -47.
Camara-Zapata, J. M., Nieves, M., Cerda, A. (2003): Improvement in growth and salt resistance of lemon (Citrus limon) trees by an interstock-induced mechanism. Tree Physiol., 23, 879–888.
Cerda A. , 'Improvement in growth and salt resistance of lemon (Citrus limon) trees by an interstock-induced mechanism ' (2003 ) 23 Tree Physiol. : 879 -888.
Dubey, A. K., Srivastav, M., Singh. R., Pandey, R. N., Deshmukh, P. S. (2006): Response of mango (Mangifera indica L.) genotypes to graded levels of salt stress. Indian J. Agric. Sci., 76, 670–672.
Deshmukh P. S. , 'Response of mango (Mangifera indica L.) genotypes to graded levels of salt stress ' (2006 ) 76 Indian J. Agric. Sci. : 670 -672.
Fletcher, R. A., Hofstra, G. (1988): Triazoles as potential plant protectants. In: Berg, D., Plempel, M. (eds.), Sterol Synthesis Inhibitors in Plant Protection. Ellis Hordwood Ltd., Cambridge, pp. 321–331.
Hofstra G. , '', in Sterol Synthesis Inhibitors in Plant Protection , (1988 ) -.
Fridovich, I. (1975): Superoxide dismutase. Annu. Rev. Biochem., 44, 147–159.
Fridovich I. , 'Superoxide dismutase ' (1975 ) 44 Annu. Rev. Biochem. : 147 -159.
Garcia-Sanchez, F., Carvajal, M., Sanchez-Pina, M. A., Martinez, V. (2000): Salinity resistance of Citrus seedlings in relation to hydraulic conductance, plasma membrane ATPase and anatomy of the roots. J. Plant Physiol., 156, 724–730.
Martinez V. , 'Salinity resistance of Citrus seedlings in relation to hydraulic conductance, plasma membrane ATPase and anatomy of the roots ' (2000 ) 156 J. Plant Physiol. : 724 -730.
Gaines, T. P., Parker, B. M., Gascho, G. L. (1984): Automated determination of chloride in soil and plant tissue by sodium nitrate extraction. Agron. J., 76, 371–374.
Gascho G. L. , 'Automated determination of chloride in soil and plant tissue by sodium nitrate extraction ' (1984 ) 76 Agron. J. : 371 -374.
Hajihashemi, S., Kiarostami, K. (2007): Effect of paclobutrazol and salt stress on growth and ionic contents in two cultivars of wheat. Pakistan J. Biol. Sci., 10, 41–48.
Kiarostami K. , 'Effect of paclobutrazol and salt stress on growth and ionic contents in two cultivars of wheat ' (2007 ) 10 Pakistan J. Biol. Sci. : 41 -48.
Hala, K., Mona, M., Eleiwa, E. (2008): Effect of the growth regulator uniconazole and salt stress on growth, yield and nutrients content of Ammi majus L. plant. Aust. J. Basic Appl. Sci., 2, 458–465.
Eleiwa E. , 'Effect of the growth regulator uniconazole and salt stress on growth, yield and nutrients content of Ammi majus L. plant ' (2008 ) 2 Aust. J. Basic Appl. Sci. : 458 -465.
Hare, P. D., Cress, W. A. (1997): Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul., 21, 79–102.
Cress W. A. , 'Metabolic implications of stress-induced proline accumulation in plants ' (1997 ) 21 Plant Growth Regul. : 79 -102.
Hassan, M. M., El-Azayem, A. I. A. (1990): Differences in salt tolerance of some fruit species. Egypt. J. Hortic., 17, 1–8.
El-Azayem A. I. A. , 'Differences in salt tolerance of some fruit species ' (1990 ) 17 Egypt. J. Hortic. : 1 -8.
Hiscox, J. D., Israelstam, G. F. (1979): A method for extraction of chlorophyll from leaf tissue with maceration. Can. J. Bot., 57, 1332–1334.
Israelstam G. F. , 'A method for extraction of chlorophyll from leaf tissue with maceration ' (1979 ) 57 Can. J. Bot. : 1332 -1334.
Jackson, M. L. (1980): Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd., New Delhi. 452 p.
Jackson M. L. , '', in Soil Chemical Analysis , (1980 ) -.
Jaggard, K., Lawrence, D. K., Biscoe, P. V. (1982): An understanding of crop physiology in assessing a plant growth regulator on sugar beet. In: McLaren, J. S. (ed.), Chemical Manipulation of Crop Growth and Development. Butterworth Ltd., London, pp. 139–150.
Biscoe P. V. , '', in Chemical Manipulation of Crop Growth and Development , (1982 ) -.
Jaleel, C. A., Kishorekumar. A., Manivannan, P., Sankar, B., Gomathinayagam, M., Gopi, R., Somasundaram, R., Panneerselvam, R., (2007a): Alterations in carbohydrate metabolism and enhancement in tuber production in white yam (Dioscorea rotundata Poir.) under triadimefon and hexaconazole applications. Plant Growth Regul., 53, 7–16.
Panneerselvam R. , 'Alterations in carbohydrate metabolism and enhancement in tuber production in white yam (Dioscorea rotundata Poir.) under triadimefon and hexaconazole applications ' (2007 ) 53 Plant Growth Regul. : 7 -16.
Jaleel, C. A., Manivannan, P., Kishorekumar, A., Sankar, B., Panneerselvam, R., Gopi, R., Somsundaram, R., Panneerselvam, R. (2007c): Induction of drought stress tolerance by ketoconazole in Catharanthus roseus is mediated by enhanced antioxidant potential and secondary metabolite accumulation. Colloids Surf, B Biointerfaces, 60, 201–206.
Panneerselvam R. , 'Induction of drought stress tolerance by ketoconazole in Catharanthus roseus is mediated by enhanced antioxidant potential and secondary metabolite accumulation ' (2007 ) 60 Colloids Surf, B Biointerfaces : 201 -206.
Jaleel, C. A., Manivannan, P., Lakshmanan, G. M. A., Sridharan, R., Panneerselvam, R. (2007b): NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus. C. R. Biologies, 330, 806–813.
Panneerselvam R. , 'NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus ' (2007 ) 330 C. R. Biologies : 806 -813.
Jamalian, S., Tehranifar, A., Tafazoli, E., Eshghi, S., Davarynejad, G. H. (2008): Paclobutrazol application ameliorates the negative effect of salt stress on reproductive growth, yield, and fruit quality of strawberry plants. Hortic. Environ. Biotech., 49, 203–208.
Davarynejad G. H. , 'Paclobutrazol application ameliorates the negative effect of salt stress on reproductive growth, yield, and fruit quality of strawberry plants ' (2008 ) 49 Hortic. Environ. Biotech. : 203 -208.
Kabi, T., Pujari, R. (1980): Effect of IAA, GA3 and CCC at different levels of inorganic phosphate on rooting of Ipomoea batas L. leaves, Geobios, 7, 170–171.
Pujari R. , 'Effect of IAA, GA3 and CCC at different levels of inorganic phosphate on rooting of Ipomoea batas L. leaves ' (1980 ) 7 Geobios : 170 -171.
Kent, L. M., Lauchli, A. (1985): Germination and seedling growth of cotton: salinity-calcium interactions. Plant Cell Environ., 8, 155–159.
Lauchli A. , 'Germination and seedling growth of cotton: salinity-calcium interactions ' (1985 ) 8 Plant Cell Environ. : 155 -159.
Kishor, A., Shrivastav, M., Dubey, A. K., Singh, A. K., Sairam, R. K., Pandey, R. N., Dahuja, A., Sharma, R. R. (2009): Paclobutrazol minimizes salt stress in mango (Mangifera indica L.). J. Hortic. Sci. Biotechnol., 84, 459–465.
Sharma R. R. , 'Paclobutrazol minimizes salt stress in mango (Mangifera indica L.) ' (2009 ) 84 J. Hortic. Sci. Biotechnol. : 459 -465.
Kraus, T. E., Fletcher, R. A. (1994): Paclobutrazol protects wheat seedlings from heat and paraquat injury. Is detoxification of active oxygen involved? Plant Cell Physiol., 35, 45–52.
Fletcher R. A. , 'Paclobutrazol protects wheat seedlings from heat and paraquat injury. Is detoxification of active oxygen involved? ' (1994 ) 35 Plant Cell Physiol. : 45 -52.
Levitt, J. (1980): Responses of Plants to Environmental Stresses, Vol. 2. Academic Press, New York.
Levitt J. , '', in Responses of Plants to Environmental Stresses, Vol. 2 , (1980 ) -.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. (1951): Protein measurement with Folin reagent. J. Biol. Chem., 193, 265–275.
Randall R. J. , 'Protein measurement with Folin reagent ' (1951 ) 193 J. Biol. Chem. : 265 -275.
Luck, H. (1965): Catalase. In: Bergmeyer, H. U. (ed.), Methods of Enzymatic Analysis. Academic Press, New York, pp. 885–894.
Luck H. , '', in Methods of Enzymatic Analysis , (1965 ) -.
Mackay, C., Hall, J., Hofstra, G., Fletcher, R. (1990): Uniconasole-induced changes in abscisic acid, total amino acid and proline in Phaseolus vulgaris. Pesticides Biochem. Physiol., 37, 74–82.
Fletcher R. , 'Uniconasole-induced changes in abscisic acid, total amino acid and proline in Phaseolus vulgaris ' (1990 ) 37 Pesticides Biochem. Physiol. : 74 -82.
Manivannan, P., Jaleel, C. A., Kishorekumar, A., Sankar, B., Somasundaram, R., Sridharan, R., Panneerselvam, R. (2007): Propiconazole induced changes in antioxidant metabolism and drought stress amelioration in Vigna unguiculata (L.) Walp, Colloids Surf B: Biointerfaces, 57, 69–74.
Panneerselvam R. , 'Propiconazole induced changes in antioxidant metabolism and drought stress amelioration in Vigna unguiculata (L.) Walp ' (2007 ) 57 Colloids Surf B: Biointerfaces : 69 -74.
Munns, R., Tester, M. (2008): Mechanisms of salinity tolerance. Annu. Rev. Plant Biol., 59, 651–681.
Tester M. , 'Mechanisms of salinity tolerance ' (2008 ) 59 Annu. Rev. Plant Biol. : 651 -681.
Muthukumarasamy, M., Panneerselvam, R. (1997): Triazole induced protein metabolism in the salt stressed Raphanus sativus seedlings. J. Indian Bot. Soc., 76, 39–42.
Panneerselvam R. , 'Triazole induced protein metabolism in the salt stressed Raphanus sativus seedlings ' (1997 ) 76 J. Indian Bot. Soc. : 39 -42.
Patel, S. K., Dubey, A. K., Srivastav, M.., Singh, A. K., Dahuja, A., Pandey, R. N. (2011): Effect of NaCl concentrations in irrigation water on growth, antioxidant enzymes and nutrient acquisition in different citrus rootstocks. J. Hort. Sci. Biotech., 86, 189–195.
Pandey R. N. , 'Effect of NaCl concentrations in irrigation water on growth, antioxidant enzymes and nutrient acquisition in different citrus rootstocks ' (2011 ) 86 J. Hort. Sci. Biotech. : 189 -195.
Pinhero, R., Fletcher, R. (1994): Paclobutrazol and ancimidol protect corn seedlings from higher and low temperature stresses. Plant Growth Regul., 15, 47–53.
Fletcher R. , 'Paclobutrazol and ancimidol protect corn seedlings from higher and low temperature stresses ' (1994 ) 15 Plant Growth Regul. : 47 -53.
Sankhla, N., Davis, T. D., Upadhyaya, A., Sankhla, D., Walser, R. H., Smith, B. N. (1985): Growth and metabolism of soybean as affected by paclobutrazol. Plant Cell Physiol., 26, 913–921.
Smith B. N. , 'Growth and metabolism of soybean as affected by paclobutrazol ' (1985 ) 26 Plant Cell Physiol. : 913 -921.
Shannon, M. C., Grieve, C. M. (1999): Tolerance of vegetable crops to salinity. Sci. Hort., 78, 5–38.
Grieve C. M. , 'Tolerance of vegetable crops to salinity ' (1999 ) 78 Sci. Hort. : 5 -38.
Sharma, D. K., Dubey, A. K., Srivastav, M., Singh, A. K., Sairam, R. K., Pandey, R. N. Dahuja, A., Kaur, C. (2011): Effect of putrescine and PBZ on growth, physiochemical parameters and nutrient acquisition on salt sensitive citrus rootstock Karna khatta (Citrus karna Raf.) under NaCl stress. J. Plant Growth Regul., 30, 301–311.
Kaur C. , 'Effect of putrescine and PBZ on growth, physiochemical parameters and nutrient acquisition on salt sensitive citrus rootstock Karna khatta (Citrus karna Raf.) under NaCl stress ' (2011 ) 30 J. Plant Growth Regul. : 301 -311.
Still, J. R., Pill, W. G. (2004): Growth and stress tolerance of tomato seedlings (Lycopersicon esculentum Mill.) in response to seed treatment with paclobutrazol. J. Hortic. Sci. Biotechnol., 79, 197–203.
Pill W. G. , 'Growth and stress tolerance of tomato seedlings (Lycopersicon esculentum Mill.) in response to seed treatment with paclobutrazol ' (2004 ) 79 J. Hortic. Sci. Biotechnol. : 197 -203.
Sugavanam, B. (1984): Diastereoisomers and enantiomers of paclobutrazol: their preparation and biological activity. Pestic. Sci., 15, 296–302.
Sugavanam B. , 'Diastereoisomers and enantiomers of paclobutrazol: their preparation and biological activity ' (1984 ) 15 Pestic. Sci. : 296 -302.
Syvertsen, J. P., Smith, M. L., Boman, B. J. (1993): Tree growth, mineral nutrition and nutrient leaching losses from soil of salinized citrus. Agric. Ecosyst. Environ., 45, 319–334.
Boman B. J. , 'Tree growth, mineral nutrition and nutrient leaching losses from soil of salinized citrus ' (1993 ) 45 Agric. Ecosyst. Environ. : 319 -334.
Upadhyaya, A., Sankhla, D., Davis, T. D., Sankhla, N., Smith, B. (1985): Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. J. Plant Physiol., 121, 453–461.
Smith B. , 'Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves ' (1985 ) 121 J. Plant Physiol. : 453 -461.
Wang, S. Y., Steffens, G. L. (1985): Effect of paclobutrazol on water stress-induced ethylene biosynthesis and polyamine accumulation in apple seedling leaves. Phytochemistry, 24, 2185–2190.
Steffens G. L. , 'Effect of paclobutrazol on water stress-induced ethylene biosynthesis and polyamine accumulation in apple seedling leaves ' (1985 ) 24 Phytochemistry : 2185 -2190.
Zekri, M. (1993): Seedling emergence, growth and mineral concentration of three citrus rootstocks under salt stress. J. Plant. Nutr., 16, 1555–1568.
Zekri M. , 'Seedling emergence, growth and mineral concentration of three citrus rootstocks under salt stress ' (1993 ) 16 J. Plant. Nutr. : 1555 -1568.
Zhang, P. G., Sutton, J. C., Fletcher, R. A. (1994): Paclobutrazol protects black spruce seedlings against predisposition to gray mold induced by high temperature and drought. Can. J. For. Res., 24, 1033–1038.
Fletcher R. A. , 'Paclobutrazol protects black spruce seedlings against predisposition to gray mold induced by high temperature and drought ' (1994 ) 24 Can. J. For. Res. : 1033 -1038.
Zhou, W., Leul, M. (1999): Uniconazole induced tolerance of rape plants to heat stress in relation to changes in hormonal levels, enzyme activities and lipid peroxidation. J. Plant Growth Regul., 27, 99–104.
Leul M. , 'Uniconazole induced tolerance of rape plants to heat stress in relation to changes in hormonal levels, enzyme activities and lipid peroxidation ' (1999 ) 27 J. Plant Growth Regul. : 99 -104.
Zhu, J. K. (2003): Regulation of ion homeostasis under salt stress. Curr. Opin. Plant Biol., 6, 441–445.
Zhu J. K. , 'Regulation of ion homeostasis under salt stress ' (2003 ) 6 Curr. Opin. Plant Biol. : 441 -445.
Zid, E., Grignon, C. (1985): Sodium-calcium interactions in leaves of Citrus aurantium grown in the presence of NaCl. Physiol. Veg., 23, 895–906.
Grignon C. , 'Sodium-calcium interactions in leaves of Citrus aurantium grown in the presence of NaCl ' (1985 ) 23 Physiol. Veg. : 895 -906.