Acta Agronomica Hungarica
Volume/Issue:
Volume 61: Issue 3
Functional diversity of native mesorhizobial genotypes nodulating chickpea in Indian soils of Haryana state
Authors: R. Nadwani 1 and S. Dudeja 2
View More View Less
  • 1 Patent Office Patent and Design Division Boudhik Sampda Bhawan New Delhi India
  • 2 CCS Haryana Agricultural University Department of Microbiology Hisar India
DOI:
https://doi.org/10.1556/AAgr.61.2013.3.4
Page Count:
207–217
Publication Date:
01 Sep 2013
Online Publication Date:
01 Oct 2013
Restricted access

The functional diversity of native mesorhizobial genotypes nodulating chickpea was assessed using two chickpea cultivars under pot culture conditions. The symbiotic effectiveness ratio of the mesorhizobial genotypes showed the existence of wide functional diversity among the isolates in Haryana soils. Mesorhizobial isolates belonging to the third genotype (MG III) were more efficient than the other genotypes. The majority of the isolates (94%) had intermediate effectiveness and only a very small percentage (2%) were ineffective, while 4–6% of the isolates were highly effective with a symbiotic ratio > 4.0. These included CP2381A, CP741, CP1423 and CP2437. Among all the isolates, strain CP2381A was the most efficient isolate for both cvs HC1 and HC5. The symbiotic ratio of the shoot N contents varied for both the cultivars after inoculation with mesorhizobial isolates from different districts. The most efficient chickpea mesorhizobial isolates were found to be present in soils from the Bhiwani district followed by that from RDS Farm, Hisar, but isolates from Mahendragarh, Fatehabad and Jhajjar were less efficient.

  • Andronov, E. E., Terefework, Z., Roumiantseva, M. l., Dzyubenko, N. I., Onichtchouk, O. P., Kurchak, O. N., Dresler-Nurmi, A., Young, J. P., Simarov, B. V., Lindstrom, K. (2003): Symbiotic and genetic diversity of Rhizobium galegae isolates collected from the Galega orientalis gene center in the Caucasus. Appl. Environ. Microbiol., 69, 1067–1074.

    Lindstrom K. , 'Symbiotic and genetic diversity of Rhizobium galegae isolates collected from the Galega orientalis gene center in the Caucasus ' (2003 ) 69 Appl. Environ. Microbiol. : 1067 -1074.

    • Search Google Scholar

  • Arora, D. K. (2010): Application of Microorganisms in Agriculture and Allied Sectors (AMAAS) at a Glance, National Bureau of Agriculturally Important Microorganisms. Kushmaur, P. B. No. 6, Kaithauli, Mau Nath Bhajan, 275101 (U.P.), India. Website: {rs www.nbaim.org.in url}

    Arora D. K. , '', in Application of Microorganisms in Agriculture and Allied Sectors (AMAAS) at a Glance , (2010 ) -.

    • Search Google Scholar

  • Bremner, J. M. (1965): Total nitrogen. pp. 1149–1178. In: Black, C. A. (ed.), Methods of Soil Analysis Part 2, American Society of Agronomy, Madison.

    Bremner J. M. , '', in Methods of Soil Analysis Part 2 , (1965 ) -.

  • Bromfield, E. S., Sinha, I. B., Wolynetz, M. S. (1986): Influence of location, host cultivar, and inoculation on the composition of naturalized populations of Rhizobium meliloti in Medicago sativa nodules. Appl. Environ. Microbiol., 51, 1077–1084.

    Wolynetz M. S. , 'Influence of location, host cultivar, and inoculation on the composition of naturalized populations of Rhizobium meliloti in Medicago sativa nodules ' (1986 ) 51 Appl. Environ. Microbiol. : 1077 -1084.

    • Search Google Scholar

  • Carelli, M., Gnocchi, S., Fancelli, S., Mengoni, A., Paffetti, D., Scotti, C., Bazzicalupo, M. (2000): Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils. Appl. Environ. Microbiol., 66, 4785–4789.

    Bazzicalupo M. , 'Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils ' (2000 ) 66 Appl. Environ. Microbiol. : 4785 -4789.

    • Search Google Scholar

  • Charman, N., Ballard, R. A. (2004): Burr medic (Medicago polymorpha L.) selections for improved N2 fixation with naturalized soil rhizobia. Soil Biol. Biochem., 36, 1331–1337.

    Ballard R. A. , 'Burr medic (Medicago polymorpha L.) selections for improved N2 fixation with naturalized soil rhizobia ' (2004 ) 36 Soil Biol. Biochem. : 1331 -1337.

    • Search Google Scholar

  • Chen, Q., Zhang, X., Terefework, Z., Kaijalainen, S., Li, D., Lindstrom, K. (2003): Diversity and compatibility of peanut (Arachis hypogaeo L.) bradyrhizobia and their host plants. Plant Soil, 255, 605–617.

    Lindstrom K. , 'Diversity and compatibility of peanut (Arachis hypogaeo L.) bradyrhizobia and their host plants ' (2003 ) 255 Plant Soil : 605 -617.

    • Search Google Scholar

  • Dudeja, S. S., Gupta, S. C., Majumdar, V. L., Chaudhary, P. (2007): Competitiveness of molecularly predominant and host specific chickpea rhizobia under field conditions. pp 38–52. In: Vorisek, K. et al. (eds.), Practical Solutions for Managing Optimum C and N Content in Agricultural Soils IV. International Conference 2007, held in Prague, 20–22 June. Czech University of Life Sciences and Crop Research Institute, Prague, Czech Republic.

    Chaudhary P. , '', in Practical Solutions for Managing Optimum C and N Content in Agricultural Soils IV , (2007 ) -.

    • Search Google Scholar

  • Dudeja, S. S., Khurana, A. L. (1983): Interaction between pigeonpea rhizobia and pigeonpea cultivars during different years. Zbl. Mikrobiol., 138, 293–297.

    Khurana A. L. , 'Interaction between pigeonpea rhizobia and pigeonpea cultivars during different years ' (1983 ) 138 Zbl. Mikrobiol. : 293 -297.

    • Search Google Scholar

  • Dudeja, S. S., Khurana, A. L. (1988a): Survival and competitiveness of Bradyrhizobim spp. in the rhizosphere of pigeonpea (Cajanus cajan). Biol. Fertil. Soils, 7, 63–67.

    Khurana A. L. , 'Survival and competitiveness of Bradyrhizobim spp. in the rhizosphere of pigeonpea (Cajanus cajan) ' (1988 ) 7 Biol. Fertil. Soils : 63 -67.

    • Search Google Scholar

  • Dudeja, S. S., Khurana, A. L. (1988b): Effect of high temperature on root exudation of pigeonpea-Rhizobium symbiosis. Indian J. Microbiol., 28, 193–198.

    Khurana A. L. , 'Effect of high temperature on root exudation of pigeonpea-Rhizobium symbiosis ' (1988 ) 28 Indian J. Microbiol. : 193 -198.

    • Search Google Scholar

  • Dudeja, S. S., Khurana, A. L., Sharma, P. K., Dogra, R. C., Garg, F. C. (1995): Symbiotic effectivity of hup + and hup - Rhizobium strains on mungbean and urdbean under field conditions. Indian J. Microbiol., 35, 89–194.

    Garg F. C. , 'Symbiotic effectivity of hup+ and hup-Rhizobium strains on mungbean and urdbean under field conditions ' (1995 ) 35 Indian J. Microbiol. : 89 -194.

    • Search Google Scholar

  • Dudeja, S. S., Narula, N. (2008): Molecular diversity of root nodule forming bacteria. pp. 1–24. In: Khachatourians, G. G., Arora, D. K., Rajendran, T. P., Srivastava, A. K. (eds.), Agriculturally Important Microorganisms Vol II. Academic World International, Bophal, India.

    Narula N. , '', in Agriculturally Important Microorganisms , (2008 ) -.

  • Dudeja, S. S., Nidhi, S. (2013): Molecular diversity of rhizobial and non rhizobial bacteria from nodules of cool season legumes. In: Salar et al. (eds.), Biotechnology: Emerging Trends. Proc. of International Conference on Biotechnology (ICB-2012) organized by Department of Biotechnology, CDLU, Sirsa, India. (in press)

    Nidhi S. , '', in Biotechnology: Emerging Trends , (2013 ) -.

  • Dudeja, S. S., Sheokand, S., Kumari, S. (2012): Legume root nodule development and functioning under tropics and subtropics: Perspectives and challenges. Legume Res., 35, 85–103.

    Kumari S. , 'Legume root nodule development and functioning under tropics and subtropics: Perspectives and challenges ' (2012 ) 35 Legume Res. : 85 -103.

    • Search Google Scholar

  • Dudeja, S. S., Singh, N. P., Sharma, P., Gupta, S. C., Chandra, R., Dhar, B., Bansal, R. K., Brahmaprakash, G. P., Potdukhe, S. R., Gundappagol, R. C., Gaikawad, B. G., Nagaraj, K. S. (2011): Biofertilizer technology and pulses production. pp. 43–63 In: Singh, A., Parmar, N., Kuhad, R. C. (eds.), Bioaugmentation, Biostimulation and Biocontrol. Springer Verlag. Soil Biology Series.

    Nagaraj K. S. , '', in Bioaugmentation, Biostimulation and Biocontrol , (2011 ) -.

  • Dudeja, S. S., Singh, P. C. (2008): High and low nodulation in relation to molecular diversity of chickpea mesorhizobia in Indian soils Arch. Agron. Soil Sci., 54, 109–120.

    Singh P. C. , 'High and low nodulation in relation to molecular diversity of chickpea mesorhizobia in Indian soils ' (2008 ) 54 Arch. Agron. Soil Sci. : 109 -120.

    • Search Google Scholar

  • Hungria, M., Andrade, D. S., Chueire, L. M. O., Probanza, A., Guttierrez-Manero, F. J., Megias, M. (2000): Isolation and characterization of new efficient and competitive bean (Phaseolus vulgaris L.) rhizobia from Brazil. Soil Biol. Biochem., 32, 1515–1528.

    Megias M. , 'Isolation and characterization of new efficient and competitive bean (Phaseolus vulgaris L.) rhizobia from Brazil ' (2000 ) 32 Soil Biol. Biochem. : 1515 -1528.

    • Search Google Scholar

  • Khurana, A. L., Dudeja, S. S. (1981): Field population of rhizobia and response to inoculation, molybdenum and nitrogen fertilizer in pigeonpea Proceeding International Pigeonpea Workshop. Vol. II. ICRISAT, Hyderabad. 381–386.

    Dudeja S. S. , '', in Proceeding International Pigeonpea Workshop , (1981 ) -.

  • Khurana, A. L., Dudeja, S. S. (1982): Cicer-Rhizobium interaction with different chickpea culitvars under field conditions. Zbl. Mikrobiol., 137, 207–213.

    Dudeja S. S. , 'Cicer-Rhizobium interaction with different chickpea culitvars under field conditions ' (1982 ) 137 Zbl. Mikrobiol. : 207 -213.

    • Search Google Scholar

  • Khurana, A. L., Sharma, P. K., Dudeja, S. S. (1991): Influence of host, moisture and native rhizobial population on nodule occupancy in chickpea (Cicer arietinum). Zbl. Mikrobiol., 146, 137–142.

    Dudeja S. S. , 'Influence of host, moisture and native rhizobial population on nodule occupancy in chickpea (Cicer arietinum) ' (1991 ) 146 Zbl. Mikrobiol. : 137 -142.

    • Search Google Scholar

  • Kundu, S., Dudeja, S. S. (2008): Molecular diversity, predominance and effectiveness of mungbean rhizobia. Indian J. Microbiol., 48, 432–439.

    Dudeja S. S. , 'Molecular diversity, predominance and effectiveness of mungbean rhizobia ' (2008 ) 48 Indian J. Microbiol. : 432 -439.

    • Search Google Scholar

  • Laguerre, G., Louvrier, P., Allard, M. R., Amarger, N. (2003): Compatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum bv. viciae for nodulation of host legumes. Appl. Environ. Microbiol., 69, 2276–2283.

    Amarger N. , 'Compatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum bv. viciae for nodulation of host legumes ' (2003 ) 69 Appl. Environ. Microbiol. : 2276 -2283.

    • Search Google Scholar

  • Laranjo, M., Rodrigues, R., Alho, L., Oliviera, S. (2001): Rhizobia of chickpea from Southern Portugal: Symbiotic efficiency and genetic diversity. J. Appl. Microbiol., 90, 662–667.

    Oliviera S. , 'Rhizobia of chickpea from Southern Portugal: Symbiotic efficiency and genetic diversity ' (2001 ) 90 J. Appl. Microbiol. : 662 -667.

    • Search Google Scholar

  • Leung, K., Strain, S. R., de-Bruijin, F. J., Bottomley, P. J. (1994): Genotypic and phenotypic comparison of chromosomal types within an indigenous soil population of Rhizobium leguminosarum bv. trifolii. Appl. Environ. Microbiol., 60, 416–426.

    Bottomley P. J. , 'Genotypic and phenotypic comparison of chromosomal types within an indigenous soil population of Rhizobium leguminosarum bv. trifolii ' (1994 ) 60 Appl. Environ. Microbiol. : 416 -426.

    • Search Google Scholar

  • Li, Q. Q., Wang, E. T., Zhang, Y. Z., Zhang, Y. M., Tian, C. F., Sui, X. H., Chen, W. F., Chen, W. X. (2011): Diversity and biogeography of rhizobia isolated from root nodules of Glycine max grown in Hebei Province, China. Microb. Ecol., 61, 917–931.

    Chen W. X. , 'Diversity and biogeography of rhizobia isolated from root nodules of Glycine max grown in Hebei Province, China ' (2011 ) 61 Microb. Ecol. : 917 -931.

    • Search Google Scholar

  • Liu, J., Wang, E. T., Chen, W. X. (2005): Diverse rhizobia associated with woody legumes Wisteria sinensis, Cercis racemosa and Amorpha fruticosa grown in the temperate zone of China. Int. J. Syst. Evol. Microbiol., 55, 1439–1452.

    Chen W. X. , 'Diverse rhizobia associated with woody legumes Wisteria sinensis, Cercis racemosa and Amorpha fruticosa grown in the temperate zone of China ' (2005 ) 55 Int. J. Syst. Evol. Microbiol. : 1439 -1452.

    • Search Google Scholar

  • McInnes, A., Thies, J. E., Abbott, L. K., Howieson, J. G. (2004): Structure and diversity among rhizobial strains, populations and communities — a review. Soil Biol. Biochem., 36, 1295–1308.

    Howieson J. G. , 'Structure and diversity among rhizobial strains, populations and communities — a review ' (2004 ) 36 Soil Biol. Biochem. : 1295 -1308.

    • Search Google Scholar

  • Musiyiwa, A., Mpepereki, S., Giller, K. E. (2005): Symbiotic effectiveness and host ranges of indigenous rhizobia nodulating promiscuous soyabean varieties in Zimbabwean soils. Soil Biol. Biochem., 37, 1169–1176.

    Giller K. E. , 'Symbiotic effectiveness and host ranges of indigenous rhizobia nodulating promiscuous soyabean varieties in Zimbabwean soils ' (2005 ) 37 Soil Biol. Biochem. : 1169 -1176.

    • Search Google Scholar

  • Nandwani, R., Dudeja, S. S. (2009): Molecular diversity of mesorhizobia in Indian soils. J. Basic Microbiol., 49, 463–470.

    Dudeja S. S. , 'Molecular diversity of mesorhizobia in Indian soils ' (2009 ) 49 J. Basic Microbiol. : 463 -470.

    • Search Google Scholar

  • Rodrigues, C. S., Laranjo, M., Rodrigues, C. S., Laranjo, M., Oliveira, S. (2006): Effect of heat and pH stress in the growth of chickpea mesorhizobia. Curr. Microbiol., 53, 1–7.

    Oliveira S. , 'Effect of heat and pH stress in the growth of chickpea mesorhizobia ' (2006 ) 53 Curr. Microbiol. : 1 -7.

    • Search Google Scholar

  • Rodriguez-Echeverria, S., Perez-Fernandez, M. A., Vlaar, S., Finan, T. (2003): Analysis of the legume-rhizobia symbiosis in shrubs from central western Spain. J. Appl. Microbiol., 95, 1367–1374.

    Finan T. , 'Analysis of the legume-rhizobia symbiosis in shrubs from central western Spain ' (2003 ) 95 J. Appl. Microbiol. : 1367 -1374.

    • Search Google Scholar

  • Santi, C., Bogusz, D., Franche, C. (2013): Biological nitrogen fixation in non-legume plants. Ann. Bot. First published online March 10, 2013.

    Franche C. , '', in Ann. Bot. , (2013 ) -.

  • Sharma, R. S., Mohammed, A., Mishra, V., Babu, C. R. (2005): Diversity in a promiscuous group of rhizobia from three Sesbania spp. colonizing ecologically distinct habitats of the semiarid Delhi region. Res. Microbiol., 156, 57–67.

    Babu C. R. , 'Diversity in a promiscuous group of rhizobia from three Sesbania spp. colonizing ecologically distinct habitats of the semiarid Delhi region ' (2005 ) 156 Res. Microbiol. : 57 -67.

    • Search Google Scholar

  • Sheoran, A., Khurana, A. L., Dudeja, S. S. (1997): Nodulation competitiveness in the Rhizobium-chickpea nodulation variants symbiosis. Microbiol. Res., 152, 407–417.

    Dudeja S. S. , 'Nodulation competitiveness in the Rhizobium-chickpea nodulation variants symbiosis ' (1997 ) 152 Microbiol. Res. : 407 -417.

    • Search Google Scholar

  • Vincent, J. M. (1970): A Manual for the Practical Study of Root Nodule Bacteria. Blackwell, Oxford, 164 p.

    Vincent J. M. , '', in A Manual for the Practical Study of Root Nodule Bacteria , (1970 ) -.
Cover Acta Agronomica Hungarica
Acta Agronomica Hungarica
Print ISSN:
0238-0161
Online ISSN:
1588-2527
Keywords:
chickpea; nodulation; functional diversity; mesorhizobia; N2 fixation; molecular diversity