View More View Less
  • 1 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
  • 2 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
  • 3 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
Restricted access

Although angiogenesis is considered to be indispensable for continuous tumour growth, only very few studies have been published performing microvessel quantification during tumour progression. We mea- sured the tumour vascularity in different stages of rat pancreatic carcinogenesis induced by azaserine and promoted by raw soya flour-containing pancreatotrophic diet. Besides the tumour samples taken at 6 (atypical acinar cell nodules), 15 (adenomas) and 20 (localised adenocarcinomas) months after carcino- gen initiation, we also investigated 3 control groups: tumour-bearing host tissue of azaserine-treated rats and normal tissue of untreated rats kept on standard or pancreatotrophic diet. In contrast with the usual microvessel counting on hot spots, we determined microvascular surface density (SV) and volume den- sity (VV) by electron microscopic morphometry. There was no significant difference in these respect between the control groups. At month 6 after the azaserine induction SV and VV showed slight, non- significant decrease as compared to the host control. Both values remained unchanged until the 15th month and increased significantly by the 20th month. These results may indicate comparable growth rate of tumour and new microvessels in the premalignant stages of carcinogenesis while a more intense angio- genesis than tumour growth afterwards.

  • Rockwell, S., Knisley, J. P. S. (1997) Hypoxia and angiogenesis in experimental tumor models: Therapeutic implications. In Goldberg, I. D. and Rosen, E. M. (eds): Regulation of angiogenesis. Birkhäuser Verlag, Basel. pp. 335-360.

    Regulation of angiogenesis , () 335 -360.

  • Tanigawa, N., Lu, C., Mitsui, T., Miura, S. (1997) Quantitation of sinusoid-like vessels in hepatocellular carcinoma: its clinical and prognostic significance. Hepathology 26:5, 1216-1223.

    'Quantitation of sinusoid-like vessels in hepatocellular carcinoma: its clinical and prognostic significance ' () 26:5 Hepathology : 31 -1223.

    • Search Google Scholar
  • 18. Tóth, S., Nagy, K., Pálfia, Z., Réz, G. Regulation of cellular autophagic capacity: data from the azaserine-induced pancreatic tumor progression (in preparation)

  • Réz, G., Tóth, S., Pálfia, Z. (1999) Cellular autophagic capacity is highly increased in azaserine-induced premalignant atypical acinar nodule cells. Carcinogenesis 20, 1893-1898.

    'Cellular autophagic capacity is highly increased in azaserine-induced premalignant atypical acinar nodule cells ' () 20 Carcinogenesis : 31 -1898.

    • Search Google Scholar
  • Bochner, B. H., Cote, R. J., Weidner, N., Groshen, S., Chen, S. C., Skinner, D. G., Nichols, P. W. (1995) Angiogenesis in bladder cancer: relationship between microvessel density and tumour prognosis. J. Natl. Cancer Inst. 87:21, 1603-1612.

    'Angiogenesis in bladder cancer: relationship between microvessel density and tumour prognosis ' () 87:21 J. Natl. Cancer Inst. : 31 -1612.

    • Search Google Scholar
  • De Jaeger, K., Merlo, F. M., Kavanagh, M. C., Fyles, A. W., Hedley, D., Hill, R. P. (1998) Heterogeneity of tumour oxygenation: relationship to tumour necrosis, tumour size, and metastasis. Int. J. Radiat. Oncol. Biol. Phys. 42:4, 717-721.

    'Heterogeneity of tumour oxygenation: relationship to tumour necrosis, tumour size, and metastasis ' () 42:4 Int. J. Radiat. Oncol. Biol. Phys. : 31 -721.

    • Search Google Scholar
  • Folkman, J. (1985) Tumor angiogenesis. Adv. Cancer Res. 43, 175-203.

    'Tumor angiogenesis ' () 43 Adv. Cancer Res. : 31 -203.

  • Fontanini, G., Lucchi, M., Vignati, S., Mussi, A., Ciardiello, F., De Laurentiis, M., De Placido, S., Basolo, F., Angeletti, C. A., Bevilacqua, G. (1997) Angiogenesis as a prognostic indicator of survival in non-small-cell lung carcinoma: a prospective study. J. Natl. Cancer Inst. 89:12, 881-886.

    'Angiogenesis as a prognostic indicator of survival in non-small-cell lung carcinoma: a prospective study ' () 89:12 J. Natl. Cancer Inst. : 31 -886.

    • Search Google Scholar
  • Gasparini, G., Weidner, N., Maluta, S., Pozza, F., Boracchi, P., Mezzetti, M., Testolin, A., Bevilacqua, P. (1993) Intratumoral microvessel density and p53 protein: correlation with metastasis in head and neck squamous-cell carcinoma. Int. J. Cancer 55:5, 739-744.

    'Intratumoral microvessel density and p53 protein: correlation with metastasis in head and neck squamous-cell carcinoma ' () 55:5 Int. J. Cancer : 31 -744.

    • Search Google Scholar
  • Hanahan, D., Folkman, J. (1996) Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell 86, 353-364.

    'Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis ' () 86 Cell : 31 -364.

    • Search Google Scholar
  • Köhler, H. H., Barth, P. J., Siebel, A., Gerharz, E. W., Bittinger, A. (1996) Quantitative assessment of vascular surface density in renal cell carcinomas. Br. J. Urol. 42:4, 717-721.

    'Quantitative assessment of vascular surface density in renal cell carcinomas ' () 42:4 Br. J. Urol. : 31 -721.

    • Search Google Scholar
  • Barth, P. J., Weingärtner, K., Köhler, H. H., Bittinger, A. (1996) Assessment of the vascularization in prostatic carcinoma: A morphometric investigation. Hum. Pathol. 27, 1306-1310.

    'Assessment of the vascularization in prostatic carcinoma: A morphometric investigation ' () 27 Hum. Pathol. : 31 -1310.

    • Search Google Scholar
  • Beliën, J. A., Somi, S., de Jong, J. S., van Diest, P. J., Baak, J. P. (1999) Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer. J. Clin. Pathol. 52:3, 184-192.

    'Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer ' () 52:3 J. Clin. Pathol. : 31 -192.

    • Search Google Scholar
  • Longnecker, D. S. (1987) The azaserine-induced model of pancreatic carcinogenesis in rats. In: Scarpelli, D. G., Reddy, J. K. and Longnecker, D. S. (eds): Experimental pancreatic carcinogenesis. CRC Press, Boca Raton. pp. 117-132.

    Experimental pancreatic carcinogenesis , () 117 -132.

  • McGuinness, E. E., Hopwood, D., Wormsley, K. G. (1982) Further studies of the effects of raw soya flour on the rat pancreas. Scand. J. Gastroenterol. 17, 273-277.

    'Further studies of the effects of raw soya flour on the rat pancreas ' () 17 Scand. J. Gastroenterol. : 31 -277.

    • Search Google Scholar
  • Milgross, C. G., Tucker, S. L., Mason, K. A., Hunter, N. R., Peters, L. J., Milas, L. (1997) The effect of tumor size on necrosis and polarographically measured pO2. Acta Oncol. 36:2, 183-189.

    'The effect of tumor size on necrosis and polarographically measured pO2 ' () 36:2 Acta Oncol. : 31 -189.

    • Search Google Scholar
  • Porschen, R., Classen, S., Piontek, M., Borchard, F. (1994) Vascularization of carcinomas of the esophagus and its correlation with tumor proliferation. Cancer Res. 54:2, 587-591.

    'Vascularization of carcinomas of the esophagus and its correlation with tumor proliferation ' () 54:2 Cancer Res. : 31 -591.

    • Search Google Scholar
  • American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. J. Nutr. 107, 1340-1348.

    'Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies ' () 107 J. Nutr. : 31 -1348.

    • Search Google Scholar
  • Vermeulen, P. B., Libura, M., Libura, J., O'Neill, P. J., van Dam, P., van Marck, E., van Oosterom, A. T., Dirix, L. Y. (1997) Influence of investigator experience and microscopic field size on microvessel density in node-negative breast carcinoma. Breast Cancer Res. Treat. 42:2, 165-172.

    'Influence of investigator experience and microscopic field size on microvessel density in node-negative breast carcinoma ' () 42:2 Breast Cancer Res. Treat. : 31 -172.

    • Search Google Scholar
  • Weibel, E. R. (1969) Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol. 26, 235-302.

    'Stereological principles for morphometry in electron microscopic cytology ' () 26 Int. Rev. Cytol. : 31 -302.

    • Search Google Scholar
  • Weidner, N., Folkman, J., Pozza, F., Bevilacqua, P., Allred, E. N., Moore, D. H., Meli, S., Gasparini, G. (1992) Tumour angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J. Natl. Cancer Inst. 84:24, 1875-1887.

    'Tumour angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma ' () 84:24 J. Natl. Cancer Inst. : 31 -1887.

    • Search Google Scholar
  • Yager, J. D., Roebuck, B. D., Zurlo, J., Longnecker, D. S., Weselcouch, E. O., Wilpone, S. A. (1981) A single-dose protocol for azaserine initiation of pancreatic carcinogenesis in the rat. Int. J. Cancer 28, 601-606.

    'A single-dose protocol for azaserine initiation of pancreatic carcinogenesis in the rat ' () 28 Int. J. Cancer : 31 -606.

    • Search Google Scholar