Acta Biologica Hungarica
Volume/Issue:
Volume 52: Issue 4
LOCALIZATION OF A CUTICULAR PROTEIN DURING THE POSTEMBRYONAL DEVELOPMENT OF MANDUCA SEXTA
Authors:
KINGA Molnár Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.

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NOÉMI H. Borhegyi Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.

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GY. Csikós Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.

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M. Sass Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.

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Pages:
457–471
Online Publication Date:
22 Jul 2005
Publication Date:
01 Aug 2001
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.52.2001.4.10
Restricted access

The pattern of cuticle protein synthesis by the epidermis of insects changes during the last larval, pupal and adult development, leading to an alteration in cuticular stucture and feature. We have isolated a pro- tein that had an apparent molecular mass of 33.1 kD from larval cuticle of Manduca sexta. Synthesis, transport and accumulation of MsCP33.1 were followed during metamorphosis by immunoblots and immunocytochemical methods using the antibody developed against this protein. Our data prove that the presence of MsCP33.1 in the larval cuticle is general while its appearance in the pupal or adult integu- ment is restricted only in the cuticle of wings and apodemes. We established that the synthesis of 33.1 kD protein is negatively regulated by moulting hormone (20-hydroxyecdysone). Possible roles for this cuticular protein are discussed.

  • Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-485.

    'Cleavage of structural proteins during the assembly of the head of bacteriophage T4 ' () 227 Nature : 31 -485 .

    • Search Google Scholar

  • Lampe, J. D., Willis, J. H. (1994) Characterization of a cDNA and gene encoding a cuticular protein from rigid cuticles of the giant silkmoth Hyalophora cecropia. Insect Biochem. Molec. Biol. 24, 419-435.

    'Characterization of a cDNA and gene encoding a cuticular protein from rigid cuticles of the giant silkmoth Hyalophora cecropia ' () 24 Insect Biochem. Molec. Biol. : 31 -435 .

    • Search Google Scholar

  • Locke, M., Kiss, A., Sass, M. (1994) The cuticular localization of integument peptides from particular routing categories. Tissue Cell 26, 707-734.

    'The cuticular localization of integument peptides from particular routing categories ' () 26 Tissue Cell : 31 -734 .

    • Search Google Scholar

  • Marmaras, V. J., Charalambidis, N. D., Zervas, Ch. G. (1996) Immune response in insects: the role of phenoloxydase in defense reactions in relation to melanization and sclerotization. Arch. Insect Biochem. Phys. 31, 119-133.

    'Immune response in insects: the role of phenoloxydase in defense reactions in relation to melanization and sclerotization ' () 31 Arch. Insect Biochem. Phys. : 31 -133 .

    • Search Google Scholar

  • Apple, R. T, Fristrom, J. W. (1991) 20-hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes. Dev. Biol. 146, 569-582.

    '20-hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes ' () 146 Dev. Biol. : 31 -582 .

    • Search Google Scholar

  • Bell, R. A., Joachim, F. G. (1976) Techniques for rearing laboratory colonies of the tobacco hornworm and pink bollworms. Ann, Entomol. Soc. Am. 69, 365-373.

    'Techniques for rearing laboratory colonies of the tobacco hornworm and pink bollworms ' () 69 Ann, Entomol. Soc. Am. : 31 -373 .

    • Search Google Scholar

  • Binger, L. C., Willis, J. H. (1990) In vitro translation of epidermal RNA from different anatomical regions metamorphic stages. Insect Biochem. 20, 573-583.

    'In vitro translation of epidermal RNA from different anatomical regions metamorphic stages ' () 20 Insect Biochem. : 31 -583 .

    • Search Google Scholar

  • Binger, L. S., Willis, J. H. (1994) Identification of the cDNA, gene and promoter for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia. Insect Biochem. Molec. Biol. 24, 989-1000.

    'Identification of the cDNA, gene and promoter for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia ' () 24 Insect Biochem. Molec. Biol. : 31 -1000 .

    • Search Google Scholar

  • Charles, J.-P., Bouhin, H., Quennedey, B., Current, A., Delachambre, J. (1992) CDNA cloning and deduced amino acid sequence of a major, glycine-rich cuticular protein from the coleopteran Tenebrio molitor. Temporal and spatial distribution of the transcript during metamorphosis. Eur. J. Biochem. 206, 813-819.

    'CDNA cloning and deduced amino acid sequence of a major, glycine-rich cuticular protein from the coleopteran Tenebrio molitor. Temporal and spatial distribution of the transcript during metamorphosis ' () 206 Eur. J. Biochem. : 31 -819 .

    • Search Google Scholar

  • Cox, D. C., Willis, J. H. (1985) The cuticular proteins of Hyalophora cecropia from different anatomical regions and metamorphotic stages. Insect Biochem. 146, 349-362.

    'The cuticular proteins of Hyalophora cecropia from different anatomical regions and metamorphotic stages ' () 146 Insect Biochem. : 31 -362 .

    • Search Google Scholar

  • Csikós, Gy., Molnár, K., Borhegyi, H. N., Talián, Cs. G., Sass, M. (1999) Insect cuticle, an in vivo model of protein trafficking. J. Cell Science 112, 2113-2124.

    'Insect cuticle, an in vivo model of protein trafficking ' () 112 J. Cell Science : 31 -2124 .

    • Search Google Scholar

  • Del Castillo, P., Llorente, A. R., Stockert, J. C. (1989) Influence of fixation, axciting light and section thickness on the primary fluorescence of samples for microfluorometric analysis. Basic Appl. Histochem. 33, 251-257.

    'Influence of fixation, axciting light and section thickness on the primary fluorescence of samples for microfluorometric analysis ' () 33 Basic Appl. Histochem. : 31 -257 .

    • Search Google Scholar

  • Hiruma, K., Hardie, J., Riddiford, L. M. (1991) Hormonal regulation of epidermal metamorphosis in vitro, control of expression of a larval-specific cuticle gene. Dev. Biol. 144, 369-378.

    'Hormonal regulation of epidermal metamorphosis in vitro, control of expression of a larval-specific cuticle gene ' () 144 Dev. Biol. : 31 -378 .

    • Search Google Scholar

  • Hopkins, T. L., Krchma, L. J., Ahmad, S. A., Kramer, K. J. (2000) Pupal cuticle of Manduca sexta: characterization and profiles during sclerotization. Insect Biochem. Mol. Biol. 30, 19-27.

    'Pupal cuticle of Manduca sexta: characterization and profiles during sclerotization ' () 30 Insect Biochem. Mol. Biol. : 31 -27 .

    • Search Google Scholar

  • Fechtel, K., Fristrom, D. K., Fristrom, J. W. (1989) Prepupal differentiation in Drosophila: distinct cell types elaborate a shared structure, the pupal cuticle, but accumulate transcriptsin unique patterns. Development 106, 649-456.

    'Prepupal differentiation in Drosophila: distinct cell types elaborate a shared structure, the pupal cuticle, but accumulate transcriptsin unique patterns ' () 106 Development : 31 -456 .

    • Search Google Scholar

  • Hawkes, R. (1982) Identification of Concanavalin A binding sites after sodium dodecyl sulphate-gel electrophoresis and protein blotting. Anal. Biochem. 123, 143-146.

    'Identification of Concanavalin A binding sites after sodium dodecyl sulphate-gel electrophoresis and protein blotting ' () 123 Anal. Biochem. : 31 -146 .

    • Search Google Scholar

  • Horodyski, F. M., Riddiford, L. M. (1989) Expression and hormonal control of a new larval cuticular multigene family at the onset of metamorphosis of the tobacco hornworm. Dev. Biol. 132, 292-303.

    'Expression and hormonal control of a new larval cuticular multigene family at the onset of metamorphosis of the tobacco hornworm ' () 132 Dev. Biol. : 31 -303 .

    • Search Google Scholar

  • Jensen, U. G., Rothman, A., Skou, L., Andersen, S. O., Roepstorff, P., Hojrup, P. (1997) Cuticular proteins from the giant cockroach, Blaberus cranifer. Insect Biochem. Molec. Biol. 27, 109-120.

    'Cuticular proteins from the giant cockroach ' () 27 Blaberus cranifer. Insect Biochem. Molec. Biol. : 31 -120 .

    • Search Google Scholar

  • Kiely, M. L., Riddiford, L. M. (1985) Temporal programming of epidermal cell protein synthesis during the larval-pupal transformation of Manduca sexta. Roux's Arch. Dev. Biol. 194, 325-335.

    'Temporal programming of epidermal cell protein synthesis during the larval-pupal transformation of Manduca sexta ' () 194 Roux's Arch. Dev. Biol. : 31 -335 .

    • Search Google Scholar

  • Koeppe, J. K., Gilbert, L. I. (1973) Immunochemical evidence for the transport of hemolymph protein into the cuticle of Manduca sexta. J. Insect Phys. 19, 615-624.

    'Immunochemical evidence for the transport of hemolymph protein into the cuticle of Manduca sexta ' () 19 J. Insect Phys. : 31 -624 .

    • Search Google Scholar

  • Missios, S., Davidson, H. C., Linder, D., Mortimer, L., Okobi, A. O., Doctor, J. S. (2000) Characterization of cuticular proteins in the red flour beetle, Tribolium castaneum. Insect Biochem. Mol. Biol. 30, 47-56.

    'Characterization of cuticular proteins in the red flour beetle ' () 30 Tribolium castaneum. Insect Biochem. Mol. Biol. : 31 -56 .

    • Search Google Scholar

  • Nakato, H., Izumi, S., Tamino, S. (1992) Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori. Biochem, Biophys. Acta 1132, 161-167.

    'Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori ' () 1132 Biochem, Biophys. Acta : 31 -167 .

    • Search Google Scholar

  • Palli, S. R., Locke, M. (1987) The synthesis of hemolymph proteins by the larval epidermis of an insect Calpodes ethlius (Lepidoptera: hesperidae). Insect Biochem. 17, 711-722.

    'The synthesis of hemolymph proteins by the larval epidermis of an insect Calpodes ethlius (Lepidoptera: hesperidae) ' () 17 Insect Biochem. : 31 -722 .

    • Search Google Scholar

  • Rebers, J., Riddiford, L. M. (1988) Structure and expression of a Manduca sexta larval cuticle gene homologous to Drosophila cuticle genes. J. Mol. Biol. 203, 411-423.

    'Structure and expression of a Manduca sexta larval cuticle gene homologous to Drosophila cuticle genes ' () 203 J. Mol. Biol. : 31 -423 .

    • Search Google Scholar

  • Riddiford, L. M. (1978) Ecdysone-induced change in cellular commitment of the epidermis of the tobacco hornworm Manduca sexta, at the initiation of metamorphosis. Gen. Comp. Endocrinol. 34, 438-446.

    'Ecdysone-induced change in cellular commitment of the epidermis of the tobacco hornworm Manduca sexta, at the initiation of metamorphosis ' () 34 Gen. Comp. Endocrinol. : 31 -446 .

    • Search Google Scholar

  • Riddiford, L. M., Baeckmann, A., Hice, R. H., Rebers, J. (1986) Developmental expression of three genes for larval cuticular proteins of the tobacco hornworm, Manduca sexta. Dev. Biol. 118, 82-94.

    'Developmental expression of three genes for larval cuticular proteins of the tobacco hornworm ' () 118 Manduca sexta. Dev. Biol. : 31 -94 .

    • Search Google Scholar

  • Sass, M., Kiss, A., Locke, M. (1994) Integument and hemocyte peptides. J. Insect Physiol. 40, 407-421.

    'Integument and hemocyte peptides ' () 40 J. Insect Physiol. : 31 -421 .

  • Sass, M., Löw, P., Móricz, Zs., Csikós, G., Kovács, J. (1991) The fate and possible role of arylphoryn during the metamorphosis of Mamestra brassicae. Acta Biol. Hung. 42, 141-160.

    'The fate and possible role of arylphoryn during the metamorphosis of Mamestra brassicae ' () 42 Acta Biol. Hung. : 31 -160 .

    • Search Google Scholar

  • Samuels, R. I., Reynolds, S. E. (1993) Moulting fluid enzymes of the tobacco hornworm, Manduca sexta: timing of proteolytic: and chinolytic activity in relation to pre-ecdysial development. Arch. Insect Phys. Biochem. 24, 33-44.

    'Moulting fluid enzymes of the tobacco hornworm, Manduca sexta: timing of proteolytic: and chinolytic activity in relation to pre-ecdysial development ' () 24 Arch. Insect Phys. Biochem. : 31 -44 .

    • Search Google Scholar

  • Sass, M., Kiss, A., Locke, M. (1993) Classes of integument peptides, Insect Biochem. Molec. Biol. 23, 845-857.

    'Classes of integument peptides ' () 23 Insect Biochem. Molec. Biol. : 31 -857 .

  • Sass, M., Kiss, A., Locke, M. (1994) The localization of surface integument peptides in tracheae and tracheoles. J. Insect Phys. 40, 561-575.

    'The localization of surface integument peptides in tracheae and tracheoles ' () 40 J. Insect Phys. : 31 -575 .

    • Search Google Scholar

  • Schenkel, H., Scheller, K. (1986) Stage and tissue specific expression of the genesencoding, Calliphorin, the major larval serum protein of Calliphora vicina. Roux's Archs. Dev. Biol. 195, 290-295.

    'Stage and tissue specific expression of the genesencoding, Calliphorin, the major larval serum protein of Calliphora vicina. Roux's Archs ' () 195 Dev. Biol. : 31 -295 .

    • Search Google Scholar

  • Snyder, M., Hunkapiller, M., Yuen, D., Silvert, D., Fristrom, J., Davidson, N. (1982) Cuticle protein genes in Drosophila. Structure, organization and evolution of four clustered genes. Cell, 29, 1024-1040.

    'Cuticle protein genes in Drosophila. Structure, organization and evolution of four clustered genes ' () 29 Cell : 31 -1040 .

    • Search Google Scholar

  • Stiles, B., Leopold, R. A. (1990) Cuticle proteins from the Anthonomus grandis abdomen: stage specificity and immunological relatedness. Insect Biochem. 20, 113-125.

    'Cuticle proteins from the Anthonomus grandis abdomen: stage specificity and immunological relatedness ' () 20 Insect Biochem. : 31 -125 .

    • Search Google Scholar

  • Towbin, H., Staehelin, T., Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.

    'Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications ' () 76 Proc. Natl. Acad. Sci. USA : 31 -4354 .

    • Search Google Scholar

  • Wielgus, J. J. (1983) Stimulation of intermoult cuticle deposition by a hemolymph trophic factor of the tobacco hornworm, Manduca sexta. Insect Biochem. 13, 313-322.

    'Stimulation of intermoult cuticle deposition by a hemolymph trophic factor of the tobacco hornworm ' () 13 Manduca sexta. Insect Biochem. : 31 -322 .

    • Search Google Scholar

  • Wielgus, J. J., Caldwell, G. A., Nichols, R. L., White, C. F. (1990) Purification, properties, and titer of hemolymph trophic factor in larvae and pupae of Manduca sexta. Insect Biochem. 20, 65-72.

    'Purification, properties, and titer of hemolymph trophic factor in larvae and pupae of Manduca sexta ' () 20 Insect Biochem. : 31 -72 .

    • Search Google Scholar

  • Wolfgang, W. J., Fristrom, D., Fristrom, J. W. (1986) The pupal cuticle of Drosophila: differential ultrastructural immunolocalization of cuticle proteins. J. Cell Biol. 102, 306-311.

    'The pupal cuticle of Drosophila: differential ultrastructural immunolocalization of cuticle proteins ' () 102 J. Cell Biol. : 31 -311 .

    • Search Google Scholar

  • Wolfgang, W. J., Riddiford, L. M. (1986) Larval cuticular morphogenesis in the tobacco hornworm, Manduca sexta, and its hormonal regulation. Dev. Biol. 113, 305-316.

    'Larval cuticular morphogenesis in the tobacco hornworm, Manduca sexta, and its hormonal regulation ' () 113 Dev. Biol. : 31 -316 .

    • Search Google Scholar

  • Willis, J. H., Reiger, J. C., DeBrunner, B. A. (1981) The metamorphosis of arthropodin. In: Bhaskaran, G., Friedmann, S., Rodrigez, J. G. (eds) Current topics in insect endocrinology and nutrition. Plenum Press, New York, pp. 26-46.

    Current topics in insect endocrinology and nutrition , () 26 -46 .

  • Willis, J. H. (1996) Metamorphosis of the cuticle, its proteins, and their genes. In: Gilbert, L. I., Tate, J. R., Atkinson, B. G. (eds) Metamorphosis: Postembryonic reprogramming of gene expression in amphibian and insect cells. Academic Press, Inc., San Diego, pp. 254-282.

    Metamorphosis: Postembryonic reprogramming of gene expression in amphibian and insect cells , () 254 -282 .

    • Search Google Scholar

  • Anderson, S. O., Rafn, K., Krogh, T. N., Hojrup, P., Roepstorff, P. (1995) Comparison of larval and pupal cuticular proteins in Tenebrio molitor. Insect Biochem. Molec. Biol. 25. 177-187.

    'Comparison of larval and pupal cuticular proteins in Tenebrio molitor ' () 25 Insect Biochem. Molec. Biol. : 31 -187 .

    • Search Google Scholar

  • Bouhin, H., Charles, J.-P., Quennedey, B., Delachambre, J. (1992) Developmental profiles of epidermal mRNA content during the development of Bombyx mori larvae. Insect Biochem. 19, 29-39.

    'Developmental profiles of epidermal mRNA content during the development of Bombyx mori larvae ' () 19 Insect Biochem. : 31 -39 .

    • Search Google Scholar

  • Bray, T. P., Lee, W. J., Yamakawa, M., Koizumi, Y., Perrot, S., Francois, M., Ashida, M. (1993) Role of the integument in insect immunity: Epicuticular abrasion and induction of cecropin synthesis in cuticular epithelial cells. Proc. Natl. Acad. Sci. USA. 90, 6275-6279.

    'Role of the integument in insect immunity: Epicuticular abrasion and induction of cecropin synthesis in cuticular epithelial cells ' () 90 Proc. Natl. Acad. Sci. USA. : 31 -6279 .

    • Search Google Scholar
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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Year of
Foundation		 1950
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Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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