View More View Less
  • 1 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
  • | 2 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
  • | 3 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
  • | 4 Department of General Zoology, Eötvös Loránd University, Please ask the editor of the journal.
Restricted access

Scolexin is one of the bacterial induced hemolymph proteins of tobacco hornworm (Manduca sexta) lar- vae, that has hemocyte coagulation-provoking activity. The 72 kDa scolexin complex is composed of two 36 kDa subunits. To examine the protein secretory pathways in insect epithelia a polyclonal antibody was raised against the 36 kDa hemolymph protein. This MsH36 antibody recognised a 36 and a 72 kDa pro- tein in tissue homogenates. On the basis of the characteristic labelling pattern observed on immunoblots and immunocytochemical sections we concluded that the 36 kDa protein in the hemolymph, in the midgut and in the epidermis was identical with the scolexin subunit. In present paper we report a labelling shift in the midgut epithelium between goblet and columnar cells that may be controlled by the hormonal system. A 72 kDa protein showed similar epitops and molecular weight to the scolexin com- plex and was detected in epidermis and in cuticle under both reducing and non-reducing conditions. Tissue localization of 36 kDa and 72 kDa MsH36 antibody labelling proteins indicated the possibility that the epidermal cells produce two kinds of scolexin-like proteins. The complex composed of 36 kDa subunits are transported basolaterally into the circulation and display hemocyte coagulation inducing activity while the 72 kDa form contains two subunits linked covalently secreted apically into the cuticle.

  • Minnick, M. F., Spence, K. D. (1988) Tissue site and modification of a bacteria induced coagulation protein in Manduca sexta. Insect Biochem. 18, 637-644.

    'Tissue site and modification of a bacteria induced coagulation protein in Manduca sexta ' () 18 Insect Biochem. : 31 -644.

    • Search Google Scholar
  • Palli, S. R., Locke, M. (1987) The synthesis of hemolymph proteins by the larval midgut of an insect Calpodes ethlius (Lepidoptera: Hesperidae). Insect Biochem. 17, 561-572.

    'The synthesis of hemolymph proteins by the larval midgut of an insect Calpodes ethlius (Lepidoptera: Hesperidae) ' () 17 Insect Biochem. : 31 -572.

    • Search Google Scholar
  • Russell, V. W., Dunn, P. E. (1991) Lysosyme in the midgut of Manduca sexta during metamorphosis. Arch. Insect Biochem. Physiol. 17, 67-80

    'Lysosyme in the midgut of Manduca sexta during metamorphosis ' () 17 Arch. Insect Biochem. Physiol. : 31 -80.

    • Search Google Scholar
  • Samuels, R. I., Reynolds, S. E. (1993) Moulting fluid enzymes of the tobacco hornworm, Manduca sexta: timing of proteolytic and chinolytic activity in relation to pre-ecdysial development. Arch. Insect Biochem. Physiol. 24, 33-44.

    'Moulting fluid enzymes of the tobacco hornworm, Manduca sexta: timing of proteolytic and chinolytic activity in relation to pre-ecdysial development ' () 24 Arch. Insect Biochem. Physiol. : 31 -44.

    • Search Google Scholar
  • Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.

    'Cleavage of structural proteins during the assembly of the head of bacteriophage T4 ' () 227 Nature : 31 -685.

    • Search Google Scholar
  • Locke, M., Kiss, A., Sass, M. (1994) The cuticular localization of integument peptides from particular routing categories. Tissue Cell 26, 707-734.

    'The cuticular localization of integument peptides from particular routing categories ' () 26 Tissue Cell : 31 -734.

    • Search Google Scholar
  • Marcu, O., Locke, M. (1999) The origin, transport and cleavage of the moult-associated cuticular protein CECP22 from Calpodes ethlius (Lepidoptera, Hesperidae). J. Insect Physiol. 45, 861-870.

    'The origin, transport and cleavage of the moult-associated cuticular protein CECP22 from Calpodes ethlius (Lepidoptera, Hesperidae) ' () 45 J. Insect Physiol. : 31 -870.

    • Search Google Scholar
  • Minnick, M. F., Rupp, R. A., Spence, K. D. (1986) A bacterial-induced lectin which triggers hemocyte coagulation in Manduca sexta. Biochem. Biophys. Res. Comm. 137, 729-735.

    'A bacterial-induced lectin which triggers hemocyte coagulation in Manduca sexta ' () 137 Biochem. Biophys. Res. Comm. : 31 -735.

    • Search Google Scholar
  • Sass, M., Kiss, A., Locke, M. (1993) Classes of integument peptides. Insect Biochem. Mol. Biol. 23, 845-857.

    'Classes of integument peptides ' () 23 Insect Biochem. Mol. Biol. : 31 -857.

  • Towbin, H., Staehelin, T., Gordon, J. (1979) Electroforetic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application. Nat. Acad. Sci. USA 76, 4350-4354.

    'Electroforetic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application ' () 76 Nat. Acad. Sci. USA : 31 -4354.

    • Search Google Scholar
  • Willott, E., Trenczek, T., Thrower, L. W., Kanost, M. R. (1994) Immunochemical identification of insect hemocyte populations: monoclonal antibodies distinguish four major hemocyte types in Manduca sexta, Eur. J. Cell. Biol. 65, 417-423.

    'Immunochemical identification of insect hemocyte populations: monoclonal antibodies distinguish four major hemocyte types in Manduca sexta, Eur ' () 65 J. Cell. Biol. : 31 -423.

    • Search Google Scholar
  • Aronson, A. I., Geng, C., Wu, L. (1999) Aggregation of bacillus thuringiensis Cryl A toxins upon binding to target insect larval midgut vesicles. Appl. Environ. Microbiol. 65, 2503-2507.

    'Aggregation of bacillus thuringiensis Cryl A toxins upon binding to target insect larval midgut vesicles ' () 65 Appl. Environ. Microbiol. : 31 -2507.

    • Search Google Scholar
  • Borhegyi, H. N., Molnár, K., Csikós, Gy., Sass, M. (1999) Isolation and characterisation of an apically sorted 41 kDa protein from the midgut of tobacco hornworm (Manduca sexta): Cell Tissue Res. 297, 513-525.

    'Isolation and characterisation of an apically sorted 41 kDa protein from the midgut of tobacco hornworm ' () 297 (Manduca sexta): Cell Tissue Res. : 31 -525.

    • Search Google Scholar
  • Cioffi, M. (1979) The morphology and fine structure of the larval mindgut of a moth (Manduca sexta) in relation to active ion transport. Tissue Cell 11, 467-479.

    'The morphology and fine structure of the larval mindgut of a moth ' () 11 (Manduca sexta) : 31 -479.

    • Search Google Scholar
  • Csikós, Gy., Molnár, K., Borhegyi, H. N., Talián, Cs. G., Sass, M. (1999) Insect cuticle, an in vivo model of protein trafficking. J. Cell Sci. 112, 2113-2124.

    'Insect cuticle, an in vivo model of protein trafficking ' () 112 J. Cell Sci. : 31 -2124.

    • Search Google Scholar
  • Finnerty, C. M., Granados, R. R. (1997) The plasma protein scolexin from Manduca sexta is induced by baculovirus infection and other immune challenges. Insect Biochem. Mol. Biol. 27, 1-7.

    'The plasma protein scolexin from Manduca sexta is induced by baculovirus infection and other immune challenges ' () 27 Insect Biochem. Mol. Biol. : 31 -7.

    • Search Google Scholar
  • Finnerty, C. M., Granados, R. R., Hughes, P. R., Belotti, A. C. (1994) Bioassay of several baculoviruses for virus induced mortality in Manduca sexta larvae and induction of infection-specific protein. J. Invert. Path. 63, 140-144.

    'Bioassay of several baculoviruses for virus induced mortality in Manduca sexta larvae and induction of infection-specific protein ' () 63 J. Invert. Path. : 31 -144.

    • Search Google Scholar
  • Finnerty, C. M., Karplus, P. A., Granados, R. R. (1999) The insect immune protein scolexin is a novel serine proteinase homolog. Protein Science 8, 242-248.

    'The insect immune protein scolexin is a novel serine proteinase homolog ' () 8 Protein Science : 31 -248.

    • Search Google Scholar
  • Hust, G., Tsukamoto, K., Rasilo, M. L., Ikezawa, H. (1999) Molecular cloning of a GPI-anchored aminopeptidase N from Bombyx mori midgut: a putative receptor for Bacillus thuringiensis CryIA toxin. Gene 214, 177-185.

    'Molecular cloning of a GPI-anchored aminopeptidase N from Bombyx mori midgut: a putative receptor for Bacillus thuringiensis CryIA toxin ' () 214 Gene : 31 -185.

    • Search Google Scholar
  • Hughes, J. A., Hurlbert, R. E., Rupp, R. A., Spence, K. D. (1983) Bacteria-induced haemolymph proteins of Manduca sexta pupae and larvae. J. Insect. Physiol. 29, 625-632.

    'Bacteria-induced haemolymph proteins of Manduca sexta pupae and larvae ' () 29 J. Insect. Physiol. : 31 -632.

    • Search Google Scholar
  • Hurlbert, R. E., Karlinsey, J. E., Spence, K. D. (1985) Differential synthesis of bacteria-induced proteins of Manduca sexta larvae and pupae. J. Insect. Physiol. 31, 205-215.

    'Differential synthesis of bacteria-induced proteins of Manduca sexta larvae and pupae ' () 31 J. Insect. Physiol. : 31 -215.

    • Search Google Scholar
  • Kyriakides, T. R., McKillip, J. L., Spence, K. D. (1995) Biochemical characterization, developmental expression, and induction of the immune protein scolexin from Manduca sexta. Arch. Insect Biochem. Physiol. 29, 269-280.

    'Biochemical characterization, developmental expression, and induction of the immune protein scolexin from Manduca sexta ' () 29 Arch. Insect Biochem. Physiol. : 31 -280.

    • Search Google Scholar

Editorial Board

      Csányi, Vilmos (Göd)
      Dudits, Dénes (Szeged)
      Falus, András (Budapest)
      Fischer, Ernő (Pécs)
      Gábriel, Róbert (Pécs)
      Gulya, Károly (Szeged)
      Gulyás, Balázs (Stockholm)
      Hajós, Ferenc (Budapest)
      Hámori, József (Budapest)
      Heszky, László (Gödöllő)
      Hideg, Éva (Szeged)
      E. Ito (Sanuki)
      Janda, Tibor (Martonvásár)
      Kavanaugh, Michael P. (Missoula)
      Kása, Péter (Szeged)
      Klein, Éva (Stockholm)
      Kovács, János (Budapest)
      Brigitte Mauch-Mani (Neuchâtel)
      Nässel, Dick R. (Stockholm)
      Nemcsók, János (Szeged)
      Péczely, Péter (Gödöllő)
      Roberts, D. F. (Newcastle-upon-Tyne)
      Sakharov, Dimitri A. (Moscow)
      Singh, Meharvan (Fort Worth)
      Sipiczky, Mátyás (Debrecen)
      Szeberényi, József (Pécs)
      Székely, György (Debrecen)
      Tari, Irma (Szeged)
      Vágvölgyi, Csaba (Szeged),
      L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)