Acta Biologica Hungarica
Volume/Issue:
Volume 53: Issue 1-2
Morphological study of organotypic cerebellar cultures
Authors:
J. Takács Neurobiology Research Group, United Research Organisation of the Hungarian Academy of Sciences and Semmelweis University, Medical School Tűzoltó u. 58, H-1094 Budapest, Hungary

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and
F. Metzger Institute of Anatomy I, University of Freiburg, Neural Plasticity Research Group D-79104 Freiburg, Germany

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Pages:
187–204
Online Publication Date:
22 Jul 2005
Publication Date:
01 Mar 2002
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.53.2002.1-2.18
Keywords:
ultrastructure; ultrastructure; ultrastructure; Cerebellum; organotypic culture; synaptic plasticity
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Organotypic cerebellar cultures from 8-days-old (P8) mouse pups were studied following 11 days of in vitro (11DIV) culturing. The cerebellar cytoarchitectonic structure was maintained in most parasagittal cerebellar cortical slice cultures (also containing the deep cerebellar nuclei). The two main extrinsic excitatory inputs (the climbing and the mossy fibers) seem to be replaced by other axonal types: in the molecular layer mostly by parallel fibers (for climbing fibers) and in the granular layer by intrinsic mossy fiber collaterals of local excitatory interneurons, the unipolar brush cells. However, in a few organotypic cultures, which (although preserving the trilaminar cerebellar cortical structure) were “granuloprival” but also contained some of the deep cerebellar nuclei, the participation of extracortical axons from the deep cerebellar nuclei in the replacement of the missing afferents is suggested.

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Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Editor(s)-in-Chief: Elekes, Károly

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    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
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    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
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    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
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