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  • 1 Alzheimer's Disease Research Centre, University of Szeged H-6720 Szeged, Hungary;
  • 2 Department of Pharmaceutical Technology University of Szeged, H-6720 Szeged, Hungary
  • 3 Alzheimer's Disease Research Centre, Department of Psychiatry University of Szeged, H-6720 Szeged, Hungary
  • 4 Department of Chemistry, University of Szeged University of Szeged, H-6720 Szeged, Hungary
  • 5 Alzheimer's Disease Research Centre, Department of Psychiatry University of Szeged, H-6720 Szeged, Hungary
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The neurotoxic effect of amyloid-beta peptide (1-42) was investigated in cultures of neuronal tissue derived from the basal forebrain of embryonic rat. The axonal varicosities of the cholinergic cells were revealed by vesicular acetylcholine transporter staining, and the axonal varicosities in general by synaptophysin immunohistochemistry. The results demonstrate that the treatment of in vitro neuronal cultures with 20 mM amyloid-beta peptide (1-42) for 2 days on day 5, 12 or 15 exerted a neurotoxic effect on both the cholinergic and the non-cholinergic neurons. In the same cultures, the absolute number of synaptophysin-positive axon varicosities was reduced to greater extent (control: 203 ± 37/field vs treated: 101 ± 16/field) than the number of vesicular acetylcholine transporter-immunoreactive (control: 48 ± 4/field vs treated: 0/field) structures. It is concluded that amyloid-beta peptide (1-42) does not have a specific effect only on the cholinergic neurons, but affects non-cholinergic neurons as well.

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