View More View Less
  • 1 Department of Biochemistry, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • | 2 Department of Biochemistry, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • | 3 Department of Comparative Physiology, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • | 4 Balaton Limnological Research Institute P.O. Box 35, H-8237 Tihany, Hungary
  • | 5 Department of Biochemistry, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • | 6 Cereal Research Institute P.O. Box 391, H-6701 Szeged, Hungary
  • | 7 Department of Biochemistry, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • | 8 Department of Biochemistry, Faculty of Sciences, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
Restricted access

Humics and pesticides are present in aquatic environment and the toxicological consequences of their chemical interaction is well studied. However, data concerning the mechanism of the biochemical action of humic-pesticide combinations are scarce, especially in vertebrates. Thus we have chosen to study the in vivo effects of the plant polyphenolic tannic acid and the pyrethroid insecticide deltamethrin [Decis] alone or in combination on hepatic xenobiotic-metabolizing enzyme activities and the associated redox-parameters in carp, as the complex assessment of these systems are regarded to serve as a relevant biomarker of environmental pollution. Stress effects and tissue damage were followed by determination of the plasma glucose level, the activities of plasma transaminases, and by electron microscopy. Tannic acid alone exerted weak prooxidant effect due to its marked antioxidant enzyme inhibitory activity. Deltamethrin, applied in a very low dose, induced oxyradical production in fish via activation of cytochrome P450 isozymes. This effect was promoted by the antioxidant enzyme inhibitory action of tannic acid, when the two chemicals were combined; however, the ultrastructural damage of the hepatocytes was reduced by the common cytoprotective capacity of the phenolic. Numerous humics are known to alter the toxicity of pesticides and their influence depends on their type and concentration. Therefore, our work taken together with other comparative studies may contribute to the assessment of the impact of humics in nature, especially in case of environmental pollution.

  • Barry, M. J., O'Halloran, K., Logan, D. C., Ahokas, J. T., Holdway, D. A. (1995) Sublethal effects of esfenvalerate pulse-exposure on spawning and non-spawning Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis). Arch. Environ. Contam. Toxicol. 28, 459-463.

    'Sublethal effects of esfenvalerate pulse-exposure on spawning and non-spawning Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis). ' () 28 Arch. Environ. Contam. Toxicol. : 459 -463.

    • Search Google Scholar
  • Beers, R. F. Jr., Sizer, I. W. (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195, 133-140.

    'A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. ' () 195 Biol. Chem. : 133 -140.

    • Search Google Scholar
  • Benedeczky, I., Nemcsók, J., Halasy, K. (1986) Electronmicroscopic analysis of the cytopathologycal effect of pesticides in the liver, kidney and gill tissues of carp. Acta Biol. Szeged. 32, 69-91.

    'Electronmicroscopic analysis of the cytopathologycal effect of pesticides in the liver, kidney and gill tissues of carp. ' () 32 Acta Biol. Szeged. : 69 -91.

    • Search Google Scholar
  • Burke, M. D., Thompson, S., Elcombe, C. R., Halpert, J., Haaparanta, T., Mayer, R. T. (1985) Ethoxy-, pentoxy- and benzyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450. Biochem. Pharmacol., 34, 3337-3345.

    'Ethoxy-, pentoxy- and benzyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450 ' () 34 Biochem. Pharmacol. : 3337 -3345.

    • Search Google Scholar
  • Chiu, D. T. Y., Stults, F. H., Tappal, A. L. (1976) Purification and properties of rat lung soluble glutathione peroxidase. Biochim. Biophys. Acta 445, 558-566.

    'Purification and properties of rat lung soluble glutathione peroxidase. ' () 445 Biochim. Biophys. Acta : 558 -566.

    • Search Google Scholar
  • de Zwart, L. L., Meerman, J. H. N., Commandeur, J. N. M., Vermeulen, N. P. E. (1999) Biomarkers of free radical damage applications in experimental animals and in humans. Free Rad. Biol. Med. 26, 202-226.

    'Biomarkers of free radical damage applications in experimental animals and in humans. ' () 26 Free Rad. Biol. Med. : 202 -226.

    • Search Google Scholar
  • Decker, E. A. (1997) Phenolics: prooxidants or antioxidants? Nutr. Rev. 55, 396-398.

    'Phenolics: prooxidants or antioxidants ' () 55 Nutr. Rev. : 396 -398.

  • Gali, H. U., Perchellet, E. M., Klish, D. S., Johnson, J. M., Perchellet, J.-P. (1992) Hydrolyzable tannins: Potent inhibitors of hydroperoxide production and tumor promotion in mouse skin treated with 12-O-tetradecanoylphorbol-13-acetate in vivo. Int. J. Cancer 51, 425-432.

    'Hydrolyzable tannins: Potent inhibitors of hydroperoxide production and tumor promotion in mouse skin treated with 12-O-tetradecanoylphorbol-13-acetate in vivo. ' () 51 Int. J. Cancer : 425 -432.

    • Search Google Scholar
  • Gjessing, E. T. (1976) Physical and chemical characteristics of aquatic humus. Ann Arbour Science Publishers, Ann Arbour, MI.

  • Gupta, A., Nigam, D., Gupta, A., Shukla, G. S., Agarwal, A. K. (1999) Effect of pyrethroid-based liquid mosquito repellent inhalation on the blood-brain barrier function and oxidative damage in selected organs of developing rats. J. Appl. Toxicol. 19, 67-72.

    'Effect of pyrethroid-based liquid mosquito repellent inhalation on the blood-brain barrier function and oxidative damage in selected organs of developing rats. ' () 19 J. Appl. Toxicol. : 67 -72.

    • Search Google Scholar
  • Haslam, E. (1989) Plant polyphenols-vegetable tannins revisited. Cambridge University Press, Cambridge.

  • Kale, M., Rathore, N., John, S., Bhatnagar, D. (1999) Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicol. Lett. 105, 197-205.

    'Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. ' () 105 Toxicol. Lett. : 197 -205.

    • Search Google Scholar
  • Kamataki, T., Ando, M., Yamazoe, Y., Ishii, K., Kato, R. (1980) Sex difference in the O-dealkylation activity of 7-hydroxycoumarin O-alkyl derivatives in liver microsomes of rats. Biochem. Pharmacol. 29, 1015-1022.

    'Sex difference in the O-dealkylation activity of 7-hydroxycoumarin O-alkyl derivatives in liver microsomes of rats. ' () 29 Biochem. Pharmacol. : 1015 -1022.

    • Search Google Scholar
  • Kapadla, G. J., Paul, B. D., Chung, E. B., Ghosh, B., Pradhan, S. N. (1976) Carcinogenity of Camellia sinensis (tea) and some tannin-containig folk medicinal herbs administered subcutaneously in rats. J. Natl. Cancer Inst. 57, 207-209.

    'Carcinogenity of Camellia sinensis (tea) and some tannin-containig folk medicinal herbs administered subcutaneously in rats. ' () 57 J. Natl. Cancer Inst. : 207 -209.

    • Search Google Scholar
  • Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A.

    'A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy ' () 27 J. Cell Biol. : 137A.

    • Search Google Scholar
  • Lemaire, P., Livingstone, D. R. (1993) Pro-oxidant/antioxidant processes and organic xenibiotic interactions in marine organisms, in particular the flounder Platichthys flesus and the mussel Mytilus edulis. Trends in Comparat. Biochem. Physiol. 1, 1119-1150.

    'Pro-oxidant/antioxidant processes and organic xenibiotic interactions in marine organisms, in particular the flounder Platichthys flesus and the mussel Mytilus edulis. Trends in Comparat ' () 1 Biochem. Physiol. : 1119 -1150.

    • Search Google Scholar
  • Levine, S. L. 1998) Induction and inhibition of cytochrome P450 expression in piscine species: implications for biomarker determination and toxicant interactions. Diss. Abst. Int. Pt. B - Sci. & Eng. 58, 5266.

    'Induction and inhibition of cytochrome P450 expression in piscine species: implications for biomarker determination and toxicant interactions ' () 58 Diss. Abst. Int. Pt. B - Sci. & Eng. : 5266.

    • Search Google Scholar
  • Lowry, O. H., Rosebrongh, N. J., Farr, A. L., Randall, R. J. 1951 Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265-275.

    'Protein measurement with the Folin phenol reagent ' () 193 J. Biol. Chem. : 265 -275.

  • Metodiewa, D., Jaiswal, A. K., Cenas, N., Dickancaité, E., Segura-Aguilar, J. (1999) Quercetin may act as a cytotoxic prooxidant after its metabolic activation to semiquinone and quinoidal product. Free Rad. Biol. Med. 26, 107-116.

    'Quercetin may act as a cytotoxic prooxidant after its metabolic activation to semiquinone and quinoidal product. ' () 26 Free Rad. Biol. Med. : 107 -116.

    • Search Google Scholar
  • Deér, K. A., Banka, L., Nemcsók, J., Ábrahám, M. (1996) Effects of deltamethrin on hepatic microsomal cytochrome P450-dependent monooxygenases in carp. J. Environ. Sci. Health B 31, 637-644.

    'Effects of deltamethrin on hepatic microsomal cytochrome P450-dependent monooxygenases in carp ' () B 31 J. Environ. Sci. Health : 637 -644.

    • Search Google Scholar
  • Filippich, L. J., Zhu, J., Alsalmi, M. T. (1991) Hepatotoxic and nephrotoxic principles in Terminalia oblongata. Res. Vet. Sci. 50, 170-177.

    'Hepatotoxic and nephrotoxic principles in Terminalia oblongata ' () 50 Res. Vet. Sci. : 170 -177.

    • Search Google Scholar
  • Förlin, L. (1980) Effects of clophen A50, 3-methylcholanthrene, pregnenolone-16 alpha-carbonitrile, and phenobarbital on the hepatic microsomal cytochrome P-450-dependent monooxygenase system in rainbow trout, Salmo gairdneri, of different age and sex. Toxic. Appl. Pharmac. 54, 420-430.

    'Effects of clophen A50, 3-methylcholanthrene, pregnenolone-16 alpha-carbonitrile, and phenobarbital on the hepatic microsomal cytochrome P-450-dependent monooxygenase system in rainbow trout, Salmo gairdneri, of different age and sex. ' () 54 Toxic. Appl. Pharmac. : 420 -430.

    • Search Google Scholar
  • Misra, H. P., Fridovich, I. (1972) The role of the superoxide anion in the autooxidation of epinephrine and simple assay for superoxide dismutase. J. Biol. Chem. 247, 3170-3175.

    'The role of the superoxide anion in the autooxidation of epinephrine and simple assay for superoxide dismutase. ' () 247 J. Biol. Chem. : 3170 -3175.

    • Search Google Scholar
  • Ortego, L. S., Benson, W. H. (1992) Effects of dissolved humic material on the toxicity of selected pyrethroid insecticides. Environ. Toxicol. Chem. 11, 261-265.

    'Effects of dissolved humic material on the toxicity of selected pyrethroid insecticides. ' () 11 Environ. Toxicol. Chem. : 261 -265.

    • Search Google Scholar
  • Placer, Z. A., Cushman, L. L., Johnson, B. C. (1966) Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal. Biochem. 16, 359-364.

    'Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. ' () 16 Anal. Biochem. : 359 -364.

    • Search Google Scholar
  • Reed, J. D. (1995) Nutritional toxicology of tannins and related polyphenols in forage legumes. J. Anim. Sci. 73, 1516-1528.

    'Nutritional toxicology of tannins and related polyphenols in forage legumes. ' () 73 J. Anim. Sci. : 1516 -1528.

    • Search Google Scholar
  • Rein, H., Ristau, O., Blanck, J., Ruckpaul, K. (1992) Regulation mechanisms of xenobiotics metabolizing liver cytochrome P-450 and toxicological implications. In: Csomós, G., Fehér, J. (eds) Free radicals and the liver. Springer-Verlag, Heidelberg, pp. 29-35.

    , () 29 -35.

  • Reitman, S., Frankel, S. (1957) Determination of serum glutamic oxalacetic and glutamic pyruvic transaminanses. Am. J. Clin. Pathol. 28, 56.

    'Determination of serum glutamic oxalacetic and glutamic pyruvic transaminanses ' () 28 Am. J. Clin. Pathol. : 56.

    • Search Google Scholar
  • Sedlak, I., Lindsay, R. H. (1968) Estimation of total protein-bound and nonprotein sulfhydryl groups in the tissue with Ellman's reagent. Anal. Biochem. 25, 192-205.

    'Estimation of total protein-bound and nonprotein sulfhydryl groups in the tissue with Ellman's reagent. ' () 25 Anal. Biochem. : 192 -205.

    • Search Google Scholar
  • Sies, H. (1985) Oxidative stress. Academic Press, London.

  • Stuart, J. J., Ray, S., Harrington, B. J., Neal, J. J., Beeman, R. W. (1998) Genetic mapping of a major locus controlling pyrethroid resistance in Tribolium castaneum (Coleoptera: Tenebrionidae). J. Econ. Entomol. 9, 1232-1238.

    'Genetic mapping of a major locus controlling pyrethroid resistance in Tribolium castaneum (Coleoptera: Tenebrionidae). ' () 9 J. Econ. Entomol. : 1232 -1238.

    • Search Google Scholar
  • Trinder, P. (1969) Determination of blood glucose using an oxidase-peroxidase system with a noncarcinogenic chromogen. J. Clin. Pathol. 22, 158-161.

    'Determination of blood glucose using an oxidase-peroxidase system with a noncarcinogenic chromogen. ' () 22 J. Clin. Pathol. : 158 -161.

    • Search Google Scholar
  • Vance, R. E., Teel, R. W. (1989) Effect of tannic acid on rat liver S9 mediated mutagenesis, metabolism and DNA binding of benzo[a]pyrene. Cancer Lett. 47, 37-44.

    'Effect of tannic acid on rat liver S9 mediated mutagenesis, metabolism and DNA binding of benzo[a]pyrene. ' () 47 Cancer Lett. : 37 -44.

    • Search Google Scholar
  • Varanka, Zs., Szegletes, T., Szegletes, Zs., Nemcsók, J., Ábrahám, M. (1999) Relationship between the structure of some humic compounds and their inhibitory effects on carp catalase. Bull. Environ. Contam. Toxicol. 63, 751-758.

    'Relationship between the structure of some humic compounds and their inhibitory effects on carp catalase. ' () 63 Bull. Environ. Contam. Toxicol. : 751 -758.

    • Search Google Scholar
  • Varanka, Zs., Rojik, I., Varanka, I., Nemcsók, J., Ábrahám, M.(2001) Biochemical and morphological changes in carp (Cyprinus carpio L.) liver following exposure to copper sulfate and tannic acid. Comp. Biochem. Physiol. C, 128, 467-478.

    'Biochemical and morphological changes in carp (Cyprinus carpio L.) liver following exposure to copper sulfate and tannic acid ' () 128 Comp. Biochem. Physiol. C : 467 -478.

    • Search Google Scholar
  • Wetzel, R. G. (1993) Humic compounds from wetlands: Complexation, inactivation, and reactivation of surface-bound and extracellular enzymes. Verh. Internat. Verein. Limnol. 25, 122-128.

    'Humic compounds from wetlands: Complexation, inactivation, and reactivation of surface-bound and extracellular enzymes. ' () 25 Verh. Internat. Verein. Limnol. : 122 -128.

    • Search Google Scholar
  • Winterbourn, C. C., Metodiewa, D. (1999) Reactivity of biologically important thiol compounds with superoxide and hydrogen peroxide. Free Rad. Biol. Med. 27, 322-328.

    'Reactivity of biologically important thiol compounds with superoxide and hydrogen peroxide. ' () 27 Free Rad. Biol. Med. : 322 -328.

    • Search Google Scholar
  • World Health Organisation (WHO) (1990) Environmental Health Criteria 97: Deltamethrin. WHO, Geneva.

    , ().

  • Zitko, V., McLeese, D. W., Metcalfe, C. D., Carson, W. G. (1979) Toxicity of permethrin, decamethrin, and related pyrethroids to salmon and lobster. Bull. Environ. Contam. Toxicol. 21, 338-343.

    'Toxicity of permethrin, decamethrin, and related pyrethroids to salmon and lobster. ' () 21 Bull. Environ. Contam. Toxicol. : 338 -343.

    • Search Google Scholar
  • Baer-Dubowska, W., Szaefer, H., Krajka-Kuzniak, V. (1998) Inhibition of murine hepatic cytochrome P450 activities by natural and synthetic phenolic compounds. Xenobiotica 28, 735-743.

    'Inhibition of murine hepatic cytochrome P450 activities by natural and synthetic phenolic compounds. ' () 28 Xenobiotica : 735 -743.

    • Search Google Scholar
  • Bálint, T., Szegletes, T., Szegletes, Zs., Halasy, K., Nemcsók, J. (1995) Biochemical and subcellular changes in carp exposed to the organophosphorus methidathion and the pyrethroid deltamethrin. Aquat. Toxicol. 33, 279-295.

    'Biochemical and subcellular changes in carp exposed to the organophosphorus methidathion and the pyrethroid deltamethrin. ' () 33 Aquat. Toxicol. : 279 -295.

    • Search Google Scholar
  • Bálint, T., Ferenczy, J., Kátai, F., Kiss, I., Kráczer, L., Kufcsák, O., Láng, G., Polyhos, Cs., Szabó, I., Szegletes, T., Nemcsók, J. (1997) Similarities and differences between the massive eel (Anguilla anguilla L.) devastations that occurred in lake Balaton in 1991 and 1995. Ecotox. Environ. Saf. 37, 17-23.

    'Similarities and differences between the massive eel (Anguilla anguilla L.) devastations that occurred in lake Balaton in 1991 and 1995. ' () 37 Ecotox. Environ. Saf. : 17 -23.

    • Search Google Scholar
  • Banka, L., Deér, K. A., Nemcsók, J., Ábrahám, M. (1997) In vivo and in vitro effects of deltamethrin on cytochrome P450 monooxygenase activity in carp (Cyprinus carpio L.) liver. J. Environ. Sci. Health B 32, 789-802.

    'In vivo and in vitro effects of deltamethrin on cytochrome P450 monooxygenase activity in carp (Cyprinus carpio L.) ' () B 32 liver. J. Environ. Sci. Health : 789 -802.

    • Search Google Scholar
  • Lewis, D. F. V. (1996) Cytochromes P450, structure, function and mechanism. Taylor and Francis, Cornwall.

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)