Acta Biologica Hungarica
Volume/Issue:
Volume 53: Issue 3
Selection of strain and optimization of mutanase production in submerged cultures of Trichoderma harzianum
Authors:
A. Wiater Department of Industrial Microbiology, Maria Curie-Skłodowska University Akademicka 19, 20-033 Lublin, Poland

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J. Szczodrak Department of Industrial Microbiology, Maria Curie-Skłodowska University Akademicka 19, 20-033 Lublin, Poland

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Pages:
389–401
Online Publication Date:
22 Jul 2005
Publication Date:
01 Jul 2002
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.53.2002.3.15
Keywords:
Trichoderma harzianum; Trichoderma harzianum; Trichoderma harzianum; mutanase production; shaken flask cultures; fermenter cultures; optimization
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Nineteen fungal strains belonging to different genera were tested for extracellular mutanase production in shaken flasks. The optimal enzymatic activity was achieved by Trichoderma harzianum F-470, a strain for which the mutanase productivity has not yet been published. Some of factors affecting the enzyme production in shaken flasks and aerated fermenter cultures have been standardized. Mandels mineral medium with initial pH 5.3, containing 0.25% mutan and inoculated with 10% of the 48-h mycelium, was the best for enzyme production. A slight mutanolytic activity was also found when sucrose, raffinose, lactose and melibiose were carbon sources. Application of optimized medium and cultural conditions, as well as use of a fermenter with automatic pH control set at pH 6.0 enabled to obtain a high mutanase yield (0.33 U/ml, 2.5 U/mg protein) in a short time (2-3 days). The enzyme in crude state was stable over a pH range of 4.5-6.0, and at temperatures up to 35 °C; its maximum activity was at 40 °C and at pH 5.5.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Editor(s)-in-Chief: Elekes, Károly

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    1. Csányi, Vilmos (Göd)
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    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
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    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Foundation		 1950
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Programme		 changed title
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Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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