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  • 1 National Institute of Haematology and Immunology Diószegi u. 64, H-1113 Budapest, Hungary
  • | 2 Department of Forensic Medicine, Semmelweis University of Medicine Budapest, Hungary
  • | 3 Department of Genetics, Cell and Immunobiology Semmelweis University, Faculty of Medicine Nagyvárad tér 4, H-1089 Budapest, Hungary
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Genetic/genomic polymorphism, i.e. variations in DNA sequences are ideally assayed by direct nucleotide sequencing of a gene region or other homologous segment of the genome. An easier and cheaper approach, however, if the variants are analyzed by hybridization technology using restriction fragment length polymorphisms (RFLPs) or by detection of the number of tandem repeats (VNTR) of small DNA segments, the “minisatellites”. In this study we describe results of the DNA analysis of repetitive sequences of human 6th chromosome by the application of a chemiluminescent labeled probes. The allele frequency distribution of polymorphic DNA sequences has been determined in unrelated individuals. The isolated genomic DNA was cut with Pst I restriction enzyme, size fractionated on agarose gel and hybridized with a chemiluminescent labeled D6 S132 probe. At this locus the Pst I cleaved DNA fragments are ranging from 1841 to 6098 base pairs (bp). Specific genetic pattern was characterized by more frequent fragments (3313 and 3884 bp), and the rarely occurring ones (clustered between 1841-2595 and 5227-6098 bp). Our study provides a further possibility for characterization of individual genomic patterns.

  • Fowler, C.: Polymorphism in the major human repetitive DNA sequences: theory, practice and comparison with other methods of measuring human genome variation. In Robertson, J., Ross, A. M., Burgoyne, L. A. (eds): DNA in forensic science. Theory, techniques and applications. Ellis Horwood, 1990, pp. 87-111.

    , () 87 -111.

  • Hoelzel, A. R. (1992) Molecular genetic analysis of populations. A practical approach. Irl. Press, pp. 125-145.

    125 145

  • Jeffreys, A. J., Wilson, V., Thein, S. L. (1985) Hypervariable "minisatellite" regions in human DNA. Nature 314, 67-73.

    'Hypervariable "minisatellite" regions in human DNA ' () 314 Nature : 67 -73.

  • Nakamura, Y., Leppert, M., O'Connel, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumlin, E., White, R. (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235, 1616-1622.

    'Variable number of tandem repeat (VNTR) markers for human gene mapping ' () 235 Science : 1616 -1622.

    • Search Google Scholar
  • Baird, M., Balázs, I., Giuoti, A., Miyazaki, L., Nicholas, L., Wexler, K., Kantner, E., Glassberg, I., Allen, F., Rubinstein, P., Sussman, L. (1986) Allele frequency distribution of two highly polymorph loci. Am. J. Hum. Genet. 39, 489-501.

    'Allele frequency distribution of two highly polymorph loci ' () 39 Am. J. Hum. Genet. : 489 -501.

    • Search Google Scholar
  • Balázs, I., Baird, M., Clyne, M., Meade, E. (1989) Human population genetic studies of five hypervariable DNA loci. Am. J. Hum. Genet. 44, 182-190.

    'Human population genetic studies of five hypervariable DNA loci ' () 44 Am. J. Hum. Genet. : 182 -190.

    • Search Google Scholar
  • Farley, M. A. (1991) In: Harrington, J. J. (ed.) Forensic DNA technology. Lewis Publishers Inc., pp. 35-37 and 93-137.

    35 37

  • Krause, D., Szibor, R., Kuchhenser, W., Brückner, R.: The practical significance of human genetic RFLP-systems in paternity testing. pp. 153-159. In: Brinkmann, B. (ed.) DNA-technology and its forensic application. Springer-Verlag, pp. 153-165.

    , () 153 -165.

  • McPherson, J. D. et al. (2001) A physical map of the human genome. Nature 409, 934-941.

    'A physical map of the human genome ' () 409 Nature : 934 -941.

  • Mathew, C. G. (1991) Protocols in human molecular genetics. Humana Press, pp. 255-284.

    255 284

Editorial Board

      Csányi, Vilmos (Göd)
      Dudits, Dénes (Szeged)
      Falus, András (Budapest)
      Fischer, Ernő (Pécs)
      Gábriel, Róbert (Pécs)
      Gulya, Károly (Szeged)
      Gulyás, Balázs (Stockholm)
      Hajós, Ferenc (Budapest)
      Hámori, József (Budapest)
      Heszky, László (Gödöllő)
      Hideg, Éva (Szeged)
      E. Ito (Sanuki)
      Janda, Tibor (Martonvásár)
      Kavanaugh, Michael P. (Missoula)
      Kása, Péter (Szeged)
      Klein, Éva (Stockholm)
      Kovács, János (Budapest)
      Brigitte Mauch-Mani (Neuchâtel)
      Nässel, Dick R. (Stockholm)
      Nemcsók, János (Szeged)
      Péczely, Péter (Gödöllő)
      Roberts, D. F. (Newcastle-upon-Tyne)
      Sakharov, Dimitri A. (Moscow)
      Singh, Meharvan (Fort Worth)
      Sipiczky, Mátyás (Debrecen)
      Szeberényi, József (Pécs)
      Székely, György (Debrecen)
      Tari, Irma (Szeged)
      Vágvölgyi, Csaba (Szeged),
      L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)