Acta Biologica Hungarica
Volume/Issue:
Volume 54: Issue 2
Upregulation of mu opioid receptors by voluntary morphine administration in drinking water
Authors:
Gabriella Fábián Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences P.O. Box 521, H-6701 Szeged, Hungary

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B. Tombor Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences P.O. Box 521, H-6701 Szeged, Hungary

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Ildikó Németh Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences P.O. Box 521, H-6701 Szeged, Hungary

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Emese Kicsi G. Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences P.O. Box 521, H-6701 Szeged, Hungary

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Margit Szikszay Department of Physiology, Albert Szent-Györgyi Medical Center, Szeged University Szeged, Hungary

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Gyöngyi Horváth Department of Physiology, Albert Szent-Györgyi Medical Center, Szeged University Szeged, Hungary

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Mária Szűcs Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences P.O. Box 521, H-6701 Szeged, Hungary

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Pages:
157–166
Online Publication Date:
19 Jul 2005
Publication Date:
01 Aug 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.54.2003.2.4
Keywords:
tolerance; tolerance; tolerance; Opioid; upregulation; morphine; spinal cord
Restricted access

Morphine was provided to rats in drinking water for 21 days. Profound analgesic tolerance was detected both in hot-plate and tail-flick tests. The density of [3H]DAMGO binding sites increased by 76% in spinal cord membranes due to morphine exposure compared to those in opioid naive animals. Slightly augmented [3H]DAMGO binding was measured in the synaptic plasma membranes, with a concomitant decrease in the microsomal membranes, of morphine tolerant/dependent brains. These observations suggest that the regulation of spinal mu opioid receptors might be different from those in the brain. It is emphasized that the molecular changes underlying tolerance/dependence are influenced by several factors, such as the tissue or subcellular fractions used, besides the obvious importance of the route of drug administration. Results obtained after voluntary morphine intake further support the growing number of experimental data that chronic morphine does not internalize/downregulate the mu opioid receptors in the central nervous system.

  • Noble, F., Szűcs, M., Kieffer, B., Roques, B. P. (2000) Overexpression of dynamin is induced by chronic stimulation of mu but not delta opioid receptors: relationship with mu-related morphine dependence. Mol. Pharmacol. 58., 159-166.

    'Overexpression of dynamin is induced by chronic stimulation of mu but not delta opioid receptors: relationship with mu-related morphine dependence. ' () 58 Mol. Pharmacol. : 159 -166 .

    • Search Google Scholar

  • Paul, D., Bodnar, R. J., Gistrak, M. A., Pasternak, G. W. (1989) Different mu receptor subtypes mediate spinal and supraspinal analgesia in mice. Eur. J. Pharmacol. 168, 307-314.

    'Different mu receptor subtypes mediate spinal and supraspinal analgesia in mice. ' () 168 Eur. J. Pharmacol. : 307 -314 .

    • Search Google Scholar

  • Seevers, M. H., Deneau, G. A. (1963) Physiological aspects of tolerance and physical dependence. In: Root, W. S., Hoffmann, F. G. (eds) Physiological Pharmacology. Academic Press, New York, 565-640.

    Physiological aspects of tolerance and physical dependence. , () 565 -640 .

  • Selley, D. E., Nestler, E. J., Breivogel, C. S., Childers, S. R. (1997) Opioid receptor coupled G-proteins in rat locus coeruleus membranes: decrease in activity after chronic morphine treatment. Brain Res. 746, 10-18.

    'Opioid receptor coupled G-proteins in rat locus coeruleus membranes: decrease in activity after chronic morphine treatment. ' () 746 Brain Res. : 10 -18 .

    • Search Google Scholar

  • Szűcs, M., Coscia, C. J. (1992) Differential coupling of opioid binding sites to guanosine triphos-phate binding regulatory proteins in subcellular fractions of rat brain. J. Neurosci. Res. 31, 565-572.

    'Differential coupling of opioid binding sites to guanosine triphos-phate binding regulatory proteins in subcellular fractions of rat brain. ' () 31 J. Neurosci. Res. : 565 -572 .

    • Search Google Scholar

  • Tao, P. L., Lee, C. R., Law, P. Y., Loh, H. H. (1993) The interaction of the mu-opioid receptor and G protein is altered after chronic morphine treatment in rats. Naunyn Schmiedebergs Arch. Pharmacol. 348, 504-508.

    'The interaction of the mu-opioid receptor and G protein is altered after chronic morphine treatment in rats. ' () 348 Naunyn Schmiedebergs Arch. Pharmacol. : 504 -508 .

    • Search Google Scholar

  • Taylor, D. A., Fleming, W. W. (2000) Unifying perspectives of the mechanisms underlying the development of tolerance and physical dependence to opioids. J. Pharmacol. Exp. Therap. 297, 11-18.

    'Unifying perspectives of the mechanisms underlying the development of tolerance and physical dependence to opioids. ' () 297 J. Pharmacol. Exp. Therap. : 11 -18 .

    • Search Google Scholar

  • Tkachuk, V. A., Wollemann, M. (1979) Hypersensitivity to isoproterenol in rabbit heart decreases guanine nucleotide effect on adenylate cyclase. Biochem Pharmacol. 28, 2097-2100.

    'Hypersensitivity to isoproterenol in rabbit heart decreases guanine nucleotide effect on adenylate cyclase. ' () 28 Biochem Pharmacol. : 2097 -2100 .

    • Search Google Scholar

  • Williams, J. T., Christie, M. J., Manzoni, O. (2001) Cellular and synaptic adaptations mediating opioid dependence. Physiol. Rev. 81, 299-343.

    'Cellular and synaptic adaptations mediating opioid dependence. ' () 81 Physiol. Rev. : 299 -343 .

    • Search Google Scholar

  • Zadina, J. E., Kastin, A. J., Harrison, L. M., Chang, S. L. (1995) Opiate receptor changes after chronic exposure to agonists and antagonists. Annals N. Y. Acad. Sci. 757, 353-360.

    'Opiate receptor changes after chronic exposure to agonists and antagonists. ' () 757 Annals N. Y. Acad. Sci. : 353 -360 .

    • Search Google Scholar

  • Bradford, M. M. (1986) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal. Biochem. 72, 248-254.

    'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. ' () 72 Anal. Biochem. : 248 -254 .

    • Search Google Scholar

  • M. Christie J. J. Williams T. R. North A. (1987) Mechanisms of tolerance to opiates in locus coeruleus neurons. NIDA Res Monogr. 78, 158-168.

    'Mechanisms of tolerance to opiates in locus coeruleus neurons. ' () 78 NIDA Res Monogr. : 158 -168 .

    • Search Google Scholar

  • Craft, R. M., Stratmann, J. A., Bartok, R. E., Walpole, T. I., King, S. J. (1999) Sex differences in the development of morphine tolerance and dependence in rat. Psychopharmacology 143, 1-7.

    'Sex differences in the development of morphine tolerance and dependence in rat. ' () 143 Psychopharmacology : 1 -7 .

    • Search Google Scholar

  • Dafters, R. I., Odber, J. (1989) Effects of dose, interdose interval, and drug-signal parameters on morphine analgesic tolerance: Implications for current theories of tolerance. Behav. Neurosci. 103, 1082-1090.

    'Effects of dose, interdose interval, and drug-signal parameters on morphine analgesic tolerance: Implications for current theories of tolerance. ' () 103 Behav. Neurosci. : 1082 -1090 .

    • Search Google Scholar

  • Du, X., Skopp, G., Aderjan, R. (1999) The influence of the route of administration: a comparative study at steady state of oral sustained release morphine and morphine sulfate suppositories. Ther. Drug Monit. 21, 208-214.

    'The influence of the route of administration: a comparative study at steady state of oral sustained release morphine and morphine sulfate suppositories. ' () 21 Ther. Drug Monit. : 208 -214 .

    • Search Google Scholar

  • Duttaroy, A., Yoburn, B. C. (1995) The effect of intrinsic efficacy on opioid tolerance. Anesthesiology 82, 1226-1236.

    'The effect of intrinsic efficacy on opioid tolerance. ' () 82 Anesthesiology : 1226 -1236 .

    • Search Google Scholar

  • Fábián, G., Bozó, B., Szikszay, M., Horváth, G., Coscia, C. J., Szűcs, M. (2002) Changes in the sub-cellular distribution of mu opioid receptors and G-proteins in morphine-tolerant rat brains. J. Pharmacol. Exp. Therap. 302, 774-780.

    'Changes in the sub-cellular distribution of mu opioid receptors and G-proteins in morphine-tolerant rat brains. ' () 302 J. Pharmacol. Exp. Therap. : 774 -780 .

    • Search Google Scholar

  • Gintzler, A. R., Chakrabarti, S. (2000) Opioid tolerance and the emergence of new opioid receptor-coupled signaling. Mol. Neurobiol. 21, 21-33.

    'Opioid tolerance and the emergence of new opioid receptor-coupled signaling. ' () 21 Mol. Neurobiol. : 21 -33 .

    • Search Google Scholar

  • He, L., Fong, J., von Zastrow, M., Whistler, J. L. (2002) Regulation of opioid receptor trafficking and morphine tolerance by receptor oligomerization. Cell 108, 271-282.

    'Regulation of opioid receptor trafficking and morphine tolerance by receptor oligomerization. ' () 108 Cell : 271 -282 .

    • Search Google Scholar

  • Adams, J. U., Andrews, J. S., Hiller, J. M., Simon, E. J., Holtzman, S. G. (1987) Effects of stress and beta-funaltrexamine pretreatment on morphine analgesia and opioid binding in rats. Life Sci. 41, 2835-2844.

    'Effects of stress and beta-funaltrexamine pretreatment on morphine analgesia and opioid binding in rats. ' () 41 Life Sci. : 2835 -2844 .

    • Search Google Scholar

  • Badawy, A. A.-B., Evans, C. M., Evans, M. (1982) Production of tolerance and physical dependence in the rat by simple administration of morphine in drinking water. Br. J. Pharmac. 75, 485-491.

    'Production of tolerance and physical dependence in the rat by simple administration of morphine in drinking water. ' () 75 Br. J. Pharmac. : 485 -491 .

    • Search Google Scholar

  • Bohn, L. M., Lefkowitz, R. J., Caron, M. G. (2002) Differential mechanisms of morphine anti-nociceptive tolerance revealed in β arrestin-2 knock-out mice. J. Neurosci. 22, 10494-10500.

    'Differential mechanisms of morphine anti-nociceptive tolerance revealed in β arrestin-2 knock-out mice. ' () 22 J. Neurosci. : 10494 -10500 .

    • Search Google Scholar

  • Ingram, S. L., Vaughan, C. W., Bagley, E. E., Connor, M., Christie, M. J. (1998) Enhanced opioid efficacy in opioid dependence is caused by an altered signal transduction pathway. J. Neurosci. 18. 10269-10276.

    'Enhanced opioid efficacy in opioid dependence is caused by an altered signal transduction pathway. ' () 18 J. Neurosci. : 10269 -10276 .

    • Search Google Scholar

  • Munson, P. J., Rodbard, D. (1980) Ligand, a versatile computerized approach for characterization of ligand binding systems. Anal. Biochem. 107, 220-239.

    'Ligand, a versatile computerized approach for characterization of ligand binding systems. ' () 107 Anal. Biochem. : 220 -239 .

    • Search Google Scholar

  • Nestler, E. J., Aghajanian, G. K. (1997) Molecular and cellular basis of addiction. Science 278, 58-63.

    'Molecular and cellular basis of addiction. ' () 278 Science : 58 -63 .

  • Cox, B. M. (1993) Opioid receptor-G protein interactions: acute and chronic effects of opioids. In: Born et al. (eds) Handbook of Exp. Pharmacol. Opioids I. Springer-Verlag, London, 145-188.

    Opioid receptor-G protein interactions: acute and chronic effects of opioids. , () 145 -188 .

    • Search Google Scholar
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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
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Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

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Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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