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  • 1 Department of Anatomy and Histology, Faculty of Veterinary Science Szent István University, Budapest, Hungary
  • 2 INSERM U 128, IFR 24, Montpellier, France
  • 3 Department of Anatomy and Histology, Faculty of Veterinary Science Szent István University, Budapest, Hungary
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The dystrophin glycoprotein complex (DGC) is a membrane-associated protein complex binding extracellular matrix (ECM) molecules, such as laminin and forming a bridge towards the cytoskeleton. The molecular composition of the DGC is cell type dependent and it is involved in cell adhesion and motility. Here we present immunocytochemical localization of beta-dystroglycan, the central member of the DGC, utrophin and Dp71f, the spliced 71 kDa dystrophin protein product of the DMD gene, in cultured retinal Muller glial cells. It is shown that beta-dystroglycan and utrophin are colocalized in clusters in all parts of Muller cells including the lamellipodium and leading edge of migrating cells. As a contrast, Dp71f labels are distinct from beta-dystroglycan and confined to the perinuclear cytoplasm of Muller cells indicating that Dp71f is not a member of the DGC in cultured Muller cells.

  • Aleman, V., Osorio, B., Chavez, O., Rendon, A., Mornet, D., Martinez, D. (2001) Subcellular localization of Dp71 dystrophin isoforms in cultured hippocampal neurons and forebrain astrocytes. Histochem. Cell Biol. 115, 243-254.

    'Subcellular localization of Dp71 dystrophin isoforms in cultured hippocampal neurons and forebrain astrocytes ' () 115 Histochem. Cell Biol. : 31 -254.

    • Search Google Scholar
  • Garcia-Tovar, C. G., Luna, J., Mena, R., Soto-Zarate, C. I., Cortes, R., Perez, A., Leon-Avila, G., Mornet, D., Rendon, A., Hernandez, J. M. (2002) Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG. Acta Histochem. 104, 245-254.

    'Dystrophin isoform Dp71 is present in lamellipodia and focal complexes in human astrocytoma cells U-373 MG ' () 104 Acta Histochem. : 31 -254.

    • Search Google Scholar
  • Garcia-Tovar, C. G., Perez, A., Luna, J., Mena, R., Osorio, B., Aleman, V., Mondragon, R., Mornet, D., Rendon, A., Hernandez, J. M. (2001) Biochemical and histochemical analysis of 71 kDa dystrophin isoform (Dp71f) in rat brain. Acta Histochem. 103, 209-224.

    'Biochemical and histochemical analysis of 71 kDa dystrophin isoform (Dp71f) in rat brain ' () 103 Acta Histochem. : 31 -224.

    • Search Google Scholar
  • Gorecki, D. C., Abdulrazzak, H., Lukasiuk, K., Barnard, E. A. (1997) Differential expression of syntrophins and analysis of alternatively spliced dystrophin transcripts in the mouse brain. Eur. J. Neurosci. 9, 965-976.

    'Differential expression of syntrophins and analysis of alternatively spliced dystrophin transcripts in the mouse brain ' () 9 Eur. J. Neurosci. : 31 -976.

    • Search Google Scholar
  • Henry, M. D., Campbell, K. P. (1998) A role for dystroglycan in basement membrane assembly. Cell 95, 859-870.

    'A role for dystroglycan in basement membrane assembly ' () 95 Cell : 31 -870.

  • Claudepierre, T., Mornet, D., Pannicke, T., Forster, V., Dalloz, C., Bolanos, F., Sahel, J., Reichenbach, A., Rendon, A. (2000) Expression of Dp71 in Muller glial cells: a comparison with utrophin-and dystrophin-associated proteins. Invest. Ophthalmol. Vis. Sci. 41, 294-304.

    'Expression of Dp71 in Muller glial cells: a comparison with utrophin-and dystrophin-associated proteins ' () 41 Invest. Ophthalmol. Vis. Sci. : 31 -304.

    • Search Google Scholar
  • Claudepierre, T., Rodius, F., Frasson, M., Fontaine, V., Picaud, S., Dreyfus, H., Mornet, D., Rendon, A. (1999) Differential distribution of dystrophins in rat retina. Invest. Ophthalmol. Vis. Sci. 40, 1520-1529.

    'Differential distribution of dystrophins in rat retina ' () 40 Invest. Ophthalmol. Vis. Sci. : 31 -1529.

    • Search Google Scholar
  • Durbeej, M., Campbell, K. P. (2002) Muscular dystrophies involving the dystrophin-glycoprotein complex: an overview of current mouse models. Curr. Opin. Genet. Dev. 12, 349-361.

    'Muscular dystrophies involving the dystrophin-glycoprotein complex: an overview of current mouse models ' () 12 Curr. Opin. Genet. Dev. : 31 -361.

    • Search Google Scholar
  • Fabbrizio, E., Nudel, U., Hugon, G., Robert, A., Pons, F., Mornet, D. (1994) Characterization and localization of a 77 kDa protein related to the dystrophin gene family. Biochem. J. 299, 359-365.

    'Characterization and localization of a 77 kDa protein related to the dystrophin gene family ' () 299 Biochem. J. : 31 -365.

    • Search Google Scholar
  • Hicks, D., Courtois, Y. (1999) The growth and behaviour of rat retinal Muller cells in vitro. An improved method for isolation and culture. Exp. Eye Res. 51, 119-129.

    'The growth and behaviour of rat retinal Muller cells in vitro. An improved method for isolation and culture ' () 51 Exp. Eye Res. : 31 -129.

    • Search Google Scholar
  • Howard, P. L., Klamut, H. J., Ray, P. N. (1998) Identification of a novel actin binding site within the Dp71 dystrophin isoform. FEBS Letters 441, 337-341.

    'Identification of a novel actin binding site within the Dp71 dystrophin isoform ' () 441 FEBS Letters : 31 -341.

    • Search Google Scholar
  • Ibraghimov-Beskrovnaya, O., Ervasti, J. M., Leveille, C. J., Slaughter, C. A., Sernett, S. W., Campbell, K. P. (1992) Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix. Nature 355, 696-702.

    'Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix ' () 355 Nature : 31 -702.

    • Search Google Scholar
  • Ilsley, J. L., Sudol, M., Winder, S. J. (2001) The interaction of dystrophin with beta-dystroglycan is regulated by tyrosine phosphorylation. Cell Signal. 13, 625-632.

    'The interaction of dystrophin with beta-dystroglycan is regulated by tyrosine phosphorylation ' () 13 Cell Signal. : 31 -632.

    • Search Google Scholar
  • Ilsley, J. L., Sudol, M., Winder, S. J. (2002) The WW domain: linking cell signalling to the membrane cytoskeleton. Cell Signal. 14, 183-189.

    'The WW domain: linking cell signalling to the membrane cytoskeleton ' () 14 Cell Signal. : 31 -189.

    • Search Google Scholar
  • James, M., Nuttall, A., Ilsley, J. L., Ottersbach, K., Tinsley, J. M., Sudol, M., Winder, S. J. (2000) Adhesion-dependent tyrosine phosphorylation of (beta)-dystroglycan regulates its interaction with utrophin. J. Cell Sci. 113, 1717-1726.

    'Adhesion-dependent tyrosine phosphorylation of (beta)-dystroglycan regulates its interaction with utrophin ' () 113 J. Cell Sci. : 31 -1726.

    • Search Google Scholar
  • Leibovitz, S., Meshorer, A., Fridman, Y., Wieneke, S., Jockusch, H., Yaffe, D., Nudel, U. (2002) Exogenous Dp71 is a dominant negative competitor of dystrophin in skeletal muscle. Neuromuscul. Disord. 12, 836-844.

    'Exogenous Dp71 is a dominant negative competitor of dystrophin in skeletal muscle ' () 12 Neuromuscul. Disord. : 31 -844.

    • Search Google Scholar
  • Lumeng, C., Phelps, S., Crawford, G. E., Walden, P. D., Barald, K., Chamberlain J. S. (1999) Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases. Nat. Neurosci. 27, 611-617.

    'Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases ' () 27 Nat. Neurosci. : 31 -617.

    • Search Google Scholar
  • Méhes, E., Czirok, A., Hegedüs, B., Vicsek, T., Jancsik, V. (2002) Laminin-1 increases motility, path-searching, and process dynamism of rat and mouse Muller glial cells in vitro: implication of relationship between cell behavior and formation of retinal morphology. Cell Motil. Cytoskeleton 53, 203-213.

    'Laminin-1 increases motility, path-searching, and process dynamism of rat and mouse Muller glial cells in vitro: implication of relationship between cell behavior and formation of retinal morphology ' () 53 Cell Motil. Cytoskeleton : 31 -213.

    • Search Google Scholar
  • Pereboev, A. V., Ahmed, N., thi Man, N., Morris, G. E. (2001) Epitopes in the interacting regions of beta-dystroglycan (PPxY motif) and dystrophin (WW domain). Biochim. Biophys. Acta 1527, 54-60.

    'Epitopes in the interacting regions of beta-dystroglycan (PPxY motif) and dystrophin (WW domain) ' () 1527 Biochim. Biophys. Acta : 31 -60.

    • Search Google Scholar
  • Pons, F., Robert, A., Fabbrizio, E., Hugon, G., Califano, J. P. C., Fehrentz, J. A., Martinez, J., Mornet, D. (1994) Utrophin localization in normal and dystrophin-deficient heart. Circulation 90, 369-374.

    'Utrophin localization in normal and dystrophin-deficient heart ' () 90 Circulation : 31 -374.

    • Search Google Scholar
  • Rentschler, S., Linn, H., Deininger, K., Bedford, M. T., Espanel, X., Sudol, M. (1999) The WW domain of dystrophin requires EF-hands region to interact with beta-dystroglycan. Biol. Chem. 380, 431-142.

    'The WW domain of dystrophin requires EF-hands region to interact with beta-dystroglycan ' () 380 Biol. Chem. : 31 -142.

    • Search Google Scholar
  • Sotgia, F., Lee, H., Bedford, M. T., Petrucci, T., Sudol, M., Lisanti, M. P. (2001) Tyrosine phosphorylation of beta-dystroglycan at its WW domain binding motif, PPxY, recruits SH2 domain containing proteins. Biochemistry 40, 14585-14592.

    'Tyrosine phosphorylation of beta-dystroglycan at its WW domain binding motif, PPxY, recruits SH2 domain containing proteins ' () 40 Biochemistry : 31 -14592.

    • Search Google Scholar
  • Winder, S. J. (2001) The complexities of dystroglycan. Trends Biochem. Sci. 26, 118-124.

    'The complexities of dystroglycan ' () 26 Trends Biochem. Sci. : 31 -124.

  • Cavaldesi, M., Macchia, G., Barca, S., Defilippi, P., Tarone, G., Petrucci, T. C. (1999) Association of the dystroglycan complex isolated from bovine brain synaptosomes with proteins involved in signal transduction. J. Neurochem. 72, 1648-1655.

    'Association of the dystroglycan complex isolated from bovine brain synaptosomes with proteins involved in signal transduction ' () 72 J. Neurochem. : 31 -1655.

    • Search Google Scholar
  • Claudepierre, T., Dalloz, C., Mornet, D., Matsumura, K., Sahel, J., Rendon, A. (2000) Characterization of the intermolecular associations of the dystrophin-associated glycoprotein complex in retinal Muller glial cells. J. Cell Sci. 113, 3409-3417.

    'Characterization of the intermolecular associations of the dystrophin-associated glycoprotein complex in retinal Muller glial cells ' () 113 J. Cell Sci. : 31 -3417.

    • Search Google Scholar

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