Acta Biologica Hungarica
Volume/Issue:
Volume 54: Issue 3-4
A comparative methodical study of the faecal steroid analysis on birds: looking for a valid method of testosterone determination
Authors:
K. Kelemen Department of Reproductive Biology Szt. István University, H-2103 Gödöllő, Hungary

Search for other papers by K. Kelemen in
Current site
Google Scholar
PubMed Close
,
P. Péczely Department of Reproductive Biology Szt. István University, H-2103 Gödöllő, Hungary

Search for other papers by P. Péczely in
Current site
Google Scholar
PubMed Close
,
Zs. Szőke Department of Reproductive Biology Szt. István University, H-2103 Gödöllő, Hungary

Search for other papers by Zs. Szőke in
Current site
Google Scholar
PubMed Close
, and
V. Ladjánszky Department of Reproductive Biology Szt. István University, H-2103 Gödöllő, Hungary

Search for other papers by V. Ladjánszky in
Current site
Google Scholar
PubMed Close
Pages:
285–298
Online Publication Date:
22 Jul 2005
Publication Date:
01 Dec 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.54.2003.3-4.7
Keywords:
birds; birds; birds; testosterone; biochemistry; Steroids; faeces; methods
Restricted access

In a comparative study, a relatively simple and high sensitivity method was developed for analysis of testosterone-equivalent(s) in the faeces of different bird species. To determine the recovery of extractions and purifications, tritium-labelled testosterone was added to the wet samples. Then the samples were treated with sodium dodecil sulphate (SDS), an emulsificator to “open-up” the complex, lipid-coated particles of faecal samples. This emulsification resulted in the decrease of the quantity of interfering substances after diethyl-ether extraction and the linearity of the measured testosterone equivalents from aliquots in the range of 2 and 10 mg of faeces. In the RIA, we applied a group specific polyclonal testosterone antibody which cross-reacted with reduced metabolites and at a certain level with sulphate conjugates as well. The use of Helix enzymes did not modified significantly the results of the analysis relating to a low level of conjugated androgens in the faecal extracts. The biological validity of the method was tested on domestic cockerels, where between the plasma and faecal testosterone values a four hours phase shift was observed, with a correlation of 0.6355. This method is suitable for “non invasive”, behavioural-ethological studies.

  • Hirschenhauser, K., Möstl, E., Kotrschal, K. (1999a) Seasonal patterns of sex steroids determined from feces in different social categories of Greylag geese. Gen. Comp. Endocr. 114, 67-79.

    'Seasonal patterns of sex steroids determined from feces in different social categories of Greylag geese ' () 114 Gen. Comp. Endocr. : 31 -79 .

    • Search Google Scholar

  • Hirschenhauser, K., Möstl, E., Kotrschal, K. (1999b) Within-pair testosterone covariation and reproductive output in Greylag geese, Anser anser. Ibis 141, 577-586.

    'Within-pair testosterone covariation and reproductive output in Greylag geese ' () 141 Anser anser. Ibis : 31 -586 .

    • Search Google Scholar

  • Bishop, C. M., Hall, M. R. (1991) Non-invasing monitoring of avian reproduction by simplified fecal steroid analysis. J. Zool. London 224, 649-668.

    'Non-invasing monitoring of avian reproduction by simplified fecal steroid analysis ' () 224 J. Zool. London : 31 -668 .

    • Search Google Scholar

  • Cockrem, J. F., Rounce, J. R. (1994) Fecal measurements of oestradiol and testosterone allowed non-invasive estimation of plasma steroid concentrations in domestic fowl. Brit. Poultry Sci. 35, 433-443.

    'Fecal measurements of oestradiol and testosterone allowed non-invasive estimation of plasma steroid concentrations in domestic fowl ' () 35 Brit. Poultry Sci. : 31 -443 .

    • Search Google Scholar

  • Hirschenhauser, K., Möstl, E., Kotrschal, K. (1998) The relationship between social stress and dominance is seasonal in Greylag geese (Anser anser). Anim. Behav. 55, 171-176.

    'The relationship between social stress and dominance is seasonal in Greylag geese ' () 55 (Anser anser). Anim. Behav. : 31 -176 .

    • Search Google Scholar

  • al-Alousi, M. L., Anderson, R. A. (2002) A relatively simple and rapid multi-component method for analysis of steroid profiles in blood, fecal and liver samples. Steroids 67, 269-275.

    'A relatively simple and rapid multi-component method for analysis of steroid profiles in blood, fecal and liver samples ' () 67 Steroids : 31 -275 .

    • Search Google Scholar

  • Hirschenhauser, K., Möstl, E., Wallner, B., Dittami, J., Kotrschal, K. (2000a) Endocrine and behavioural responses of male Greylag geese (Anser anser) to pairbound challenges during the reproductive season. Ethology 106, 63-77.

    'Endocrine and behavioural responses of male Greylag geese (Anser anser) to pairbound challenges during the reproductive season ' () 106 Ethology : 31 -77 .

    • Search Google Scholar

  • Hirschenhauser, K., Möstl, E., Péczely, P., Wallner, B., Kotrschal, K. (2000b) Seasonal relationships between plasma and fecal testosterone in response to GnRH in domestic ganders. Gen. Comp. Endocr. 118, 262-272.

    'Seasonal relationships between plasma and fecal testosterone in response to GnRH in domestic ganders ' () 118 Gen. Comp. Endocr. : 31 -272 .

    • Search Google Scholar

  • Ishii, S., Wada, M., Wakabyashi, S., Sakai, H., Kubudera, Y., Yamagochi, N., Kikuchi, M. (1994) Endocrinological studies for artificial breeding of the Japanese ibis, Nipponia nippon, an endangered avian species in Asia. J. Biosci. 19, 491-502.

    'Endocrinological studies for artificial breeding of the Japanese ibis, Nipponia nippon, an endangered avian species in Asia ' () 19 J. Biosci. : 31 -502 .

    • Search Google Scholar

  • Kikuchi, M., Yamaguchi, N., Sato, F., Ishii, S. (1994) Extraction methods for fecal hormone analysis in birds. J. Ornithol. 135, 64.

    'Extraction methods for fecal hormone analysis in birds ' () 135 J. Ornithol. : 64 .

    • Search Google Scholar

  • 11. Krawny, M. (1996) Die Entwicklung einer nicht invasiven Methode zum Nachweis von Steroidhormonmetaboliten im Kot von Gansen. Dissertation, Inst. of Biochemie, Vet. Univ., Wien.

  • Péczely, P., El Halawani, M. E., Hargitai, Cs., Mézes, M., Forgó, V., Jánosi, Sz. (1993) The photorefractoriness in domestic geese: effect of gonads and thyroid ont he development of postbreeding prolactinemia. Acta Biol. Hung. 44, 329-352.

    'The photorefractoriness in domestic geese: effect of gonads and thyroid ont he development of postbreeding prolactinemia ' () 44 Acta Biol. Hung. : 31 -352 .

    • Search Google Scholar

  • Schwarzenberger, F., Möstl, E., Palme, R., Bamberg, E. (1996) Faecal steroid analysis for non-invasive monitoring of reproductive status in farm, wild and Zoo animals. Anim. Reprod. Sci. 42, 513-526.

    'Faecal steroid analysis for non-invasive monitoring of reproductive status in farm, wild and Zoo animals ' () 42 Anim. Reprod. Sci. : 31 -526 .

    • Search Google Scholar

  • Sturkie, P. D. (1976) Avian Physiology, 3rd ed., Springer Verlag, New York - Heidelberg - Berlin.

    Avian Physiology , ().

  • 17. Walpach, R. (1998) Corticosterone Ausscheidung beim Haushuhn (Gallus gallus). Dissertation, Inst. of Biochemie, Vet. Univ., Wien.

  • Wasser, S. K., Thomas, R., Nair, P. P., Guidry, C., Southers, J., Lucas, J., Wildt, D. E., Monfort, S. L., (1993) effects of dietary fibers on faecal steroid measurements in baboons (Papio cynocephalus). J. Reprod. Fert. 97, 569-574.

    'effects of dietary fibers on faecal steroid measurements in baboons ' () 97 (Papio cynocephalus). J. Reprod. Fert. : 31 -574 .

    • Search Google Scholar

  • Wasser, S. K., Bevis, K., King, G., Hanson, E. (1997) Noninvasive physiological measures of disturbance in the northern spotted owl. Conserv. Biol. 11, 1019-1022.

    'Noninvasive physiological measures of disturbance in the northern spotted owl ' () 11 Conserv. Biol. : 31 -1022 .

    • Search Google Scholar

  • Miller, W. M., Hobbs, N. T., Sousa, M. C (1991) Detectin stress responses in Rocky Mountain Bighorn sheep (Ovis canadensis canadensis) reliability of cortisol concentrations in urine and feces. Can. J. Zool. 69, 15-24.

    'Detectin stress responses in Rocky Mountain Bighorn sheep (Ovis canadensis canadensis) reliability of cortisol concentrations in urine and feces ' () 69 Can. J. Zool. : 31 -24 .

    • Search Google Scholar

  • Palme, R., Fischer, P., Schildorfer, H., Ismail, M. N. (1996) Excretion of infused 14C steroid hormones via faeces and urine in domestic livestock. Anim. Reprod. Sci. 43, 43-63.

    'Excretion of infused 14C steroid hormones via faeces and urine in domestic livestock ' () 43 Anim. Reprod. Sci. : 31 -63 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 28 0 0
Dec 2024 17 0 0
Jan 2025 42 0 0
Feb 2025 41 0 0
Mar 2025 35 0 0
Apr 2025 3 0 0
May 2025 0 0 0