Acta Biologica Hungarica
Volume/Issue:
Volume 54: Issue 3-4
Ultrastructural studies on the pheromone-producing cells in the silkmoth, Bombyx mori: formation of cytoplasmic lipid droplets before adult eclosion
Authors:
N. Yokoyama Laboratory of Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN), Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan; The University of Tokyo, Tokyo, 113-8657, Japan

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Adrien Fónagy Laboratory of Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN), Department of Zoology, Plant Protection Institute of the Hungarian Academy of Sciences Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan; P.O. Box 102, Herman Ottó u. 15, H-1525 Budapest, Hungary

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S. Tatsuki Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences The University of Tokyo, Tokyo, 113-8657, Japan

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T. Arie Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences; Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology The University of Tokyo, Tokyo, 113-8657, Japan; Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan

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S. Yamashita Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences The University of Tokyo, Tokyo, 113-8657, Japan

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S. Matsumoto Laboratory of Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN) Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan

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Pages:
299–312
Online Publication Date:
22 Jul 2005
Publication Date:
01 Dec 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.54.2003.3-4.8
Keywords:
Bombyx mori; Bombyx mori; Bombyx mori; pheromone biosynthesis; lipid droplets; basal involution; PBAN
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In Bombyx mori, pheromone-producing cells accumulate a number of lipid droplets in the cytoplasm preceding the production of the sex pheromone, bombykol. The process of lipid droplet formation in the pheromone-producing cells was investigated by using light and electron microscopy. Light microscopy revealed that the lipid droplets appeared from 2 days before adult eclosion and dramatic accumulation took place between 2 days and 1 day before eclosion. Electron microscopical studies revealed that smooth endoplasmic reticulum and numerous vesicles, their sizes being less than 1 µm, were detectable 2 days before eclosion, and some vesicles were fused with mitochondria at this stage. These characteristic changes in the pheromone-producing cells suggest that fatty acyl-CoA synthesis following de novo fatty acid synthesis takes place at this time. Involutions in the basal plasma membrane of the cells occurred throughout the observed period, which were extensive on the day before adult eclosion. Besides extensive basal involutions, immature lipid droplets appeared and then mature fully electron-dense lipid droplets were observed on the day of adult eclosion. These ultrastructural observations, combined with recent physiological studies suggest, that the basal involutions presumably reflect the uptake of lipidic components required for the construction of lipid droplets, the function of which is to store the bombykol precursor and to provide it for bombykol biosynthesis in response to pheromonotropic stimuli by pheromone biosynthesis activating neuropeptide (PBAN).

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Acta Biologica Hungarica
Language English
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