Acta Biologica Hungarica
Volume/Issue:
Volume 54: Issue 3-4
Effect of adrenalectomy on rat peritoneal macrophage response
Authors:
B. Bishayi Immunology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology 92, Acharya Prafulla Chandra Road, Calcutta 700009, India

Search for other papers by B. Bishayi in
Current site
Google Scholar
PubMed Close
,
S. Ghosh Swiss Federal Research Institute Zuercherstrasse 111, 8903 Birmensdorf, Switzerland

Search for other papers by S. Ghosh in
Current site
Google Scholar
PubMed Close
, and
P. Bhanja Immunology and Microbiology Special Paper, Department of Physiology University of Calcutta, India

Search for other papers by P. Bhanja in
Current site
Google Scholar
PubMed Close
Pages:
335–346
Online Publication Date:
22 Jul 2005
Publication Date:
01 Dec 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.54.2003.3-4.11
Keywords:
immunomodulation; immunomodulation; immunomodulation; adrenalectomy; inflammation; Glucocorticoids; rat macrophage functions
Restricted access

Glucocorticoid hormones are important for vital functions and act to modulate inflammatory and immune responses. In contrast to other hormonal systems no endogenous mediators have been identified that can directly counter-regulate their potent anti-inflammatory and immunosuppressive properties. Glucocorticoids are known to interfere with the ability of the macrophage not only to induce and amplify an immune response but also to inhibit macrophage inflammatory effector functions. Although the actual immunocompetence of animals undergoing endocrine gland ectomy has never been directly studied, there is no doubt that adrenal hormones are deeply involved in the development and maintenance of the immunitory functions and this may in turn influence the inflammatory reaction. To study the effect of endogenous glucocorticoids on the functions of rat peritoneal macrophages and induction of humoral immune response we observed some of the rat peritoneal macrophage effector functions, provided that endogenous glucocorticoids are depleted by adrenalectomy. The mean phagocytic index (PI) of control macrophage (Mf) is increased from 23,825±427 to 31,895±83 after adrenalectomy (P£0.001). Intracellular killing capacity in control cell is 82% which is found to be 73% in case of adrenalectomised cell (p<0.05). The amount of nitric oxide released from control Mf 20.25±1 mM following adrenalectomy shows the amount of nitric oxide release was 18.25 mM (p£0.01). The percentage of DNA fragmentation in control Mf was 68.82±4 which was reduced to 56.76±1 after adrenalectomy (p£0.01). In sheep red blood cell (SRBC) immunised and adrenalectomised animal, agglutination titre was obtained at lowest antibody concentration (1:128) whereas serum from SRBC immunised normal rats showed early agglutination (1:32). Endogenous glucocorticoid depleted rats show enhanced phagocytic capacity, antibody raising capacity as well as on the other hand adrenal hormone insufficiency reduces the intracellular killing capacity, nitric oxide (NO) release, improper cell maturation and heightens the probability of infection. These observations demonstrate a counter-regulatory system via glucocorticoid that functions to control inflammatory and immune responses.

  • Sakai, M., Biwa, T., Matsumura, T., Takemura, T., Matsuda, H., Anami, Y., Sasahara, T., Kobori, S., Shichiri, M. (1999) Glucocorticoids inhibits oxidized LDL-induced macrophage growth by suppressing the expression of granulocyte macrophage colony stimulating factor. Arterioscler-Thromb-Vasc. Biol. 19, 1726-1733.

    'Glucocorticoids inhibits oxidized LDL-induced macrophage growth by suppressing the expression of granulocyte macrophage colony stimulating factor ' () 19 Arterioscler-Thromb-Vasc. Biol. : 31 -1733 .

    • Search Google Scholar

  • Sasaki, S., Muira, T., Nishikawa, S., Yamada, K., Hirasue, M., Nakane, A. (1998) Protective role of nitric oxide in S. aureus infection in mice. Infect. Immun. 66, 1017-1022.

    'Protective role of nitric oxide in S. aureus infection in mice ' () 66 Infect. Immun. : 31 -1022 .

    • Search Google Scholar

  • Peers, S. H., Smillie, F., Elderfield, A. J., Flower, R. J. (1993) Glucocorticoid and non-glucocorticoid induction of lipocortins (annexins) 1 and 2 and rat peritoneal leukocytes in vivo. Br. J. Pharmacol. 108, 66-72.

    'Glucocorticoid and non-glucocorticoid induction of lipocortins (annexins) 1 and 2 and rat peritoneal leukocytes in vivo ' () 108 Br. J. Pharmacol. : 31 -72 .

    • Search Google Scholar

  • Plant, M. (1987) Lymphocyte hormone receptors. Ann. Rev. Immunol. 5, 621-669.

    'Lymphocyte hormone receptors ' () 5 Ann. Rev. Immunol. : 31 -669 .

  • Rook, G., Baker, K., Walker, B., Honour, J. (2000) Local regulation of glucocorticoid activity in sites of inflammation. Insights from the study of tuberculosis. Amer. N. Y. Acad. Sci. 917, 913-922.

    'Local regulation of glucocorticoid activity in sites of inflammation. Insights from the study of tuberculosis ' () 917 Amer. N. Y. Acad. Sci. : 31 -922 .

    • Search Google Scholar

  • Sei, T. (1992) Hormonal regulation of the immune response in stress, immunity and aging. In: E. L. Cooper (ed.) Stress, immunity and aging. Mercell Dekker Inc. New York, pp. 141-156.

    Stress, immunity and aging , () 141 -156 .

  • Shimoji, Y., Yokomizo, Y., Sekiazi, T., Mori, Y., Kubo, M. (1994) Presence of a capsule in Erysiophelothrix rhusiopathiae and its relationship to virulence for mice. Infect. Immun. 62, 2806-2810.

    'Presence of a capsule in Erysiophelothrix rhusiopathiae and its relationship to virulence for mice ' () 62 Infect. Immun. : 31 -2810 .

    • Search Google Scholar

  • Silverstein, R. (1991) Hydrazin sulfate protects D-galactosamine sensitized mice against endotoxin and tumor necrosis factor/cachectin lethality. Evidence of a role for the pituitary. J. Exp. Med. 173, 357-365.

    'Hydrazin sulfate protects D-galactosamine sensitized mice against endotoxin and tumor necrosis factor/cachectin lethality. Evidence of a role for the pituitary ' () 173 J. Exp. Med. : 31 -365 .

    • Search Google Scholar

  • Thierry, C., Jurgen, B., Christine, N. M. (1995) MIF as a glucocorticoid induced modulator of cytokine production. Nature 377, 68-71.

    'MIF as a glucocorticoid induced modulator of cytokine production ' () 377 Nature : 31 -71 .

    • Search Google Scholar

  • Vacchio, M. S., Lee, J. Y., Ashwell, J. D. (1999) Thymus derived glucocorticoids set the thresholds for thymocyte selection by inhibiting TCR mediated thymocyte activation. J. Immunol. 163, 1327-1333.

    'Thymus derived glucocorticoids set the thresholds for thymocyte selection by inhibiting TCR mediated thymocyte activation ' () 163 J. Immunol. : 31 -1333 .

    • Search Google Scholar

  • Naraba, H., Murakami, M., Matsumoto, H., Shimbara, S., Ueno, A., Kudo, I., Ohishi, S. (1998) Segregated coupling of phospholipase A2, Cyclooxygenases and terminal prostanoid synthesis in rat peritoneal macrophages. J. Immunol. 160, 2974-2982.

    'Segregated coupling of phospholipase A2, Cyclooxygenases and terminal prostanoid synthesis in rat peritoneal macrophages ' () 160 J. Immunol. : 31 -2982 .

    • Search Google Scholar

  • Nieto, M. A., Gonzalez, F., Ganbon, F., Diaz-Espada, F., Lopez-Rivas, A. (1992) Apoptosis in human monocytes after treatment with glucocorticoids. Clin. Exp. Immunol. 88, 341-344.

    'Apoptosis in human monocytes after treatment with glucocorticoids ' () 88 Clin. Exp. Immunol. : 31 -344 .

    • Search Google Scholar

  • Nishihira, J., Mitchell, R. A., Bucala, R. (2000) Macrophage migration inhibitory factor (MIF): Its essential role in the immune system and cell growth. J. Interferon. Cytokine. Res. 20, 751-762.

    'Macrophage migration inhibitory factor (MIF): Its essential role in the immune system and cell growth ' () 20 J. Interferon. Cytokine. Res. : 31 -762 .

    • Search Google Scholar

  • Ochiai, T., Fukushima, K., Ochiai, K. (1997) Butyric acid induced apoptosis of murine thymocytes splenic T-cells. Infect. Immun. 65, 35-41.

    'Butyric acid induced apoptosis of murine thymocytes splenic T-cells ' () 65 Infect. Immun. : 31 -41 .

    • Search Google Scholar

  • Vacchio, M. S., Papadopoulos, V., Ashwell, J. D. (1994) Steroid production in the thymus: implication for thymocyte selection. J. Exp. Med. 179, 1835-1846.

    'Steroid production in the thymus: implication for thymocyte selection ' () 179 J. Exp. Med. : 31 -1846 .

    • Search Google Scholar

  • Wick, G., Hu, Y., Schwarz, S. (1993) Immunoendocrine communication via the hypothalamo-pituitary adrenal axis in autoimmune diseases. Endocrine Rev. 14, 539-563.

    'Immunoendocrine communication via the hypothalamo-pituitary adrenal axis in autoimmune diseases ' () 14 Endocrine Rev. : 31 -563 .

    • Search Google Scholar

  • Wilder, R. L. (1995) Neuroendocrine-immune system interaction and autoimmunity. Ann. Rev. Immunol. 13, 307-338.

    'Neuroendocrine-immune system interaction and autoimmunity ' () 13 Ann. Rev. Immunol. : 31 -338 .

    • Search Google Scholar

  • Blalock, J. E. (1994) The syntax of immuno-neuroendocrine communication. Immunol. Today 15, 504-511.

    'The syntax of immuno-neuroendocrine communication ' () 15 Immunol. Today : 31 -511 .

    • Search Google Scholar

  • Casadevell, M., Saperas, E., Panes, J., Salas, A., Anderson, D. C., Malagelada, J. R. (1999) Mechanism underlying the anti-inflammatory action of central corticotropin releasing factor. Amer. J. Physiol. 276, G1016-1026.

    'Mechanism underlying the anti-inflammatory action of central corticotropin releasing factor ' () 276 Amer. J. Physiol. : G31 -1026 .

    • Search Google Scholar

  • Claman, H. N. (1972) Corticosteroids and lymphoid cells. N. Engl. J. Med. 287, 388-397.

    'Corticosteroids and lymphoid cells ' () 287 N. Engl. J. Med. : 31 -397 .

  • Cupps, T. R., Fanci, A. S. (1982) Corticosteroid mediated immuno regulation in man. Immunol. Rev. 65, 133-155.

    'Corticosteroid mediated immuno regulation in man ' () 65 Immunol. Rev. : 31 -155 .

  • Czuprynski, C. J., Henson P. M., Campbell, P. A. (1984) Killing of Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and non-immune mice. J. Leukocyte Biol. 35, 193-208.

    'Killing of Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and non-immune mice ' () 35 J. Leukocyte Biol. : 31 -208 .

    • Search Google Scholar

  • Da-Silva, J. A., Peers, S. H., Pereretti, M., Willoughby, D. A. (1993) Sex steroids affect glucocorticoid response to chronic inflammation and to interleukin I. J. Endocrinol. 136, 389-397.

    'Sex steroids affect glucocorticoid response to chronic inflammation and to interleukin I. J ' () 136 Endocrinol. : 31 -397 .

    • Search Google Scholar

  • Dan, G., Lall, S. B. (1998) Neuroendocrine modulation of immune system. Ind. J. Pharmacol. 30, 129-140.

    'Neuroendocrine modulation of immune system ' () 30 Ind. J. Pharmacol. : 31 -140 .

  • Gonzalo, J. A., Gonzalez-Garcia, A., Mestinez, C., Kroemer, G. (1993) Glucocorticoid mediated control of the activation and clonal deletion of peritoneal T-cells in vivo. J. Exp: Med. 177, 1239-1246.

    'Glucocorticoid mediated control of the activation and clonal deletion of peritoneal T-cells in vivo ' () 177 J. Exp: Med. : 31 -1246 .

    • Search Google Scholar

  • Gruber, J., Sgone, R., Hu, Y. H., Beng, H., Wick, G. (1994) Thymocyte apoptosis by elevated endogenous corticosterone level. Eur. J. Immunol. 24, 1115-1121.

    'Thymocyte apoptosis by elevated endogenous corticosterone level ' () 24 Eur. J. Immunol. : 31 -1121 .

    • Search Google Scholar

  • Jeffries, W. M. (1991) Cortisol and immunity. Med. Hypothesis 34, 198-208.

    'Cortisol and immunity ' () 34 Med. Hypothesis : 31 -208 .

  • Kabat, E. A., Myer, M. M. (1967) Agglutination. In: Kabat, E. A. (ed.) Experimental Immuno Chemistry, 2nd ed. 97-132.

    Experimental Immuno Chemistry , ().

  • Leigh, P. C. J., Van Furth, R., Zweet, T. L. (1986) In vitro determination of phagocytosis and intracellular killing by polymorphonuclear neutrophils and mononuclear phagocytes. In: Weir, D. M. (ed.) Handbook of Experimental Immunology. 4th ed. Blackwell Scientific Publication, Glasgow, 46.1-46.19.

    Handbook of Experimental Immunology , () 46.1 -46.19 .

  • Masferre, J. L., Reddy, S. T., Zweifel, B. S., Seibert, K., Needleman, P., Gilbert, R. S., Herschman, H. R. (1994) In vivo glucocorticoids regulate cyclooxygenase 2 but not cyclooxygenase 1 in peritoneal macrophages. J. Pharmacol. Exp. Ther. 270, 1340-1344.

    'In vivo glucocorticoids regulate cyclooxygenase 2 but not cyclooxygenase 1 in peritoneal macrophages ' () 270 J. Pharmacol. Exp. Ther. : 31 -1344 .

    • Search Google Scholar

  • Munck, A., Guyre, P. M., Holbrook, N. (1984) Physiological function of glucocorticoid in stress and their relation to pharmacological actions. Endocrine Rev. 5, 25-44.

    'Physiological function of glucocorticoid in stress and their relation to pharmacological actions ' () 5 Endocrine Rev. : 31 -44 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 36 0 0
Dec 2024 48 0 0
Jan 2025 45 0 0
Feb 2025 47 0 0
Mar 2025 51 0 0
Apr 2025 28 0 0
May 2025 0 0 0