Acta Biologica Hungarica
Volume/Issue:
Volume 54: Issue 3-4
Quantitative evaluation of macrophage phagocytosing capacity by a fluorometric assay
Authors:
Valé Miliukiené Department of Developmental Biology, Institute of Biochemistry 12 Mokslininkø Street, LT-2600, Vilnius, Lithuania

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Gené Biziuleviciené Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Laboratory of Pharmacology, Institute of Immunology University of Vilnius, 21/27 M. K. Ciurlionio Street, LT-2009, Vilnius, Lithuania, University of Vilnius, 29 Molétø plentas, LT-2021, Vilnius, Lithuania

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Audroné Pilinkiené Laboratory of Pharmacology, Institute of Immunology University of Vilnius, 29 Molétø plentas, LT-2021, Vilnius, Lithuania

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Pages:
347–356
Online Publication Date:
22 Jul 2005
Publication Date:
01 Dec 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.54.2003.3-4.12
Keywords:
yeast; yeast; yeast; Phagocytosis; macrophage; fluorometric assay
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This paper reviews sensitive and simple quantitative evaluation of macrophage phagocytosing capacity by applying fluorescein-labeled Sacharomyces cerevisiae cells. Yeast cells were conjugated with fluoresceinisothiocyanate (FITC) and used as fluorescent particles. A time course analysis within this method showed that phagocytosis of yeast cells was temperature dependent and that the number of that ones ingested by macrophages increased rapidly during the initial 60 min of incubation at 37 °C. Free fluorescent cells can be effectively removed by aspiration from the well. Furthermore, yeast cells required preopsonization with serum to achieve optimal uptake of the cells. The uptake of nonopsonized yeast cells by macrophages was significantly lower than that of opsonized cells (P<0.05). We propose that about 50% of mouse macrophages can carry functionally active FcR responsible for phagocytosis.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
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H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

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Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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