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  • 1 Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen Department of Environmental Sciences, Faculty of Science, College of Nyíregyháza Debrecen, Hungary Nyíregyháza, Hungary
  • 2 Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen Debrecen, Hungary
  • 3 Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen Debrecen, Hungary
  • 4 Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen Debrecen, Hungary
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NADPH is involved in many basically important anabolic processes. For a long time, pentose phosphate pathway (PPS) was regarded as the most important source of NADPH in fungi. Here we present evidence of a metabolic switch to an alternative NADPH-producing pathway in ageing Penicillium chrysogenumcultures, which involves NADP+-specific isocitrate dehydrogenase (NADP+-ID) rather than PPS enzymes. Considering the main biochemical functions of NADPH, we propose that NADP+-ID could have deep impact on many physiological processes switched on glucose deprivation including proteinase production or penicillin biosynthesis. We also demonstrate that although the alternative pathway was inferior to PPS when the fungus was grown on well-utilisable carbon sources yet it could have an important role in fatty acid biosynthesis as well as in the maintenance of high intracellular NADPH/NADP+ratios.

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